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N K Kovalenko

Publications and source records attributed to N K Kovalenko.

34 records · Page 2Linked to original sources
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[Study of some properties of strain Lactobacillus plantarum G3/3(13) antagonistic factor].

The screening of strains which expressed antagonistic activity, that is not connected with lactic acid and hydrogen peroxide synthesis was conducted among 72 cultures of lactic acid bacteria. The strain L. plantarum G3/3(13) which inhibited growth of 11 test-cultures including Lactobacillus plantarum, Enterococcus faecalis and E. faecium was selected. The antibacterial factor was of protein nature, did not retain the activity after boiling and was active at pH 2.0-8.0, whereas at pH 9.0 its activity fell significantly. The obtained results allow to assume that the formed agent can be bacteriocin-like substance and the strain L. plantarum G3/3(13) may be useful for food industry or probiotic construction.

Antibiosis↗
PubMedDate not supplied

[Study of vaginal and intestinal microflora of women in the prenatal period and its correction in dysbacteriosis].

A comparative research of the structure of vaginal and intestinal microflora in norm and at kidney pathologies in women in the prenatal period was carried out. At physiologically normal course of pregnancy microbiocenosis of vagina and intestine does not contain pathogenic and conditional-pathogenic microorganisms. Lactic acid bacteria are fixed within the limits of 10(5)-10(8) CFU/1 ml in the vagina secretion and 10(7)-10(8) CFU/1 g in the intestine content. The spore microorganisms have completely disappeared in vaginal biocenosis of women with pyelonephritis after pharmacotherapy; and colibacterias has disappeared in 62% of women. Quantity of lactic acid bacteria has also decreased. Quantity of E. coli (10(8)- 10(9)) CFU/g, sporous microorganisms (10(3)-10(4)) CFU/g and yeast (10(4)-10(5)) CFU/g has considerably increased in the content of intestine. Lactic acid bacteria in content of the vagina are presented by: Lactobacillus acidophilus, L. plantarum, L. fermentum, L. vaginalis; in content of the intestine--L. plantarum and L. fermentum. All the isolated strains of lactobacilli are resistant to metrogyl and amphotericin, 87%--to cephoxitine, cypropfloxacine. Twenty three of 24 strains of lactic acid bacteria--antagonists of pathogenic microflora have shown adhesion to vaginal epthelium. The degree of adhesive activity depends on the strain and genus specificity of cultures. The highest index of adhesiveness in indicated in Enterococcus faecium (5.82 - 7.62) and Streptococcus salivarius subsp. thermophilus (2.74 - 6.40) strains. The screening of strains has been performed, which suppress growth of gas-producing microflora. The strain of Lactobacillus plantarum 200D proved to be the most active one. A complex of cultures of the lactic bacteria providing normalization of microbial cenosis of the vagina in women in the prenatal period has been selected as based on the data obtained. The biotechnology of production of vaginal suppositories is developed.

Administration, Intravaginal↗
PubMedDate not supplied

[The adhesion of lactic acid bacteria to the intestinal epithelium of agricultural animals].

The lactic acid bacteria isolated from different ecological niches are established to be able to adhere in vitro to the intestine epithelium of pigs and cattle. The studied strains of enterococci have a higher index of adhesiveness (5.7-2.24) as compared to lactic acid bacilli (3.34-1.08). Detection of the lactic acid bacteria ability to adhere to intestine enterocytes of agricultural animals served as a premise to use those bacteria when constructing preparations of the directed action on the basis of living cultures.

Animal Feed↗
PubMedDate not supplied

[Lactic bacteria in the digestive tract of poultry].

Lactic bacteria predominate in the microflora of the digestive tract of chicken and turkey. They are represented mainly by Lactobacillus acidophilus, L. salivarius, L. fermentum and L. buchneri. Streptococcus faecium is always isolated. L. ruminis, L. vitulinus, L. delbrueckii, L. coryniformis and L. viridescens were found in this ecological niche for the first time. S. faecium and S. faecalis prevail in the digestive tract of geese and ducks, while lactobacilli are detected in a lesser amount and are represented mainly by L. plantarum. L. salivarius cells isolated from the digestive tract of poultry are highly polymorphous. Most of the lactic acid bacteria found in the digestive tract of poultry can grow at 45-50 degrees C whatever is the species they belong to.

Animals↗
PubMedDate not supplied

[The use of the polymerase chain reaction method for the identification of representatives of the genus Enterococcus].

The possibility of the use of repetitive extragenic palindrome (REP)-specific primers to PCR-fingerprint analysis of Enterococcus genomes has been shown for the first time. Presence of the similar PCR products, and first of all the fragment 950 bp in size, has been observed in all the strains studied. Differences in PCR-product composition displayed genetical heterogeneity between Enterococcus faecium strains. These data evidence for the wide distribution of the REP, and rather high level of homology of the REP sequences among enterobacterial genomes.

Animals↗
PubMedDate not supplied

[The lysozyme-synthetizing activity of enterococci and lactobacilli].

Lysozyme-synthesizing activity of a large collection of cultures of lactic-acid bacteria isolated from various natural substrates has been studied. It is shown that the degree of lysozyme production depends on the source of bacteria isolation. Representatives of Enterococcus genus isolated from the digestive tract of long-living people, as well as fishes, geese and ducks are most active among the coccal forms. Manifestation of lysozyme-synthesizing activity in lactobacilli depends on the group and species belonging of strains. It is established that the capacity to produce lysozyme can serve a diagnostic characteristic under identification of lactobacillus of subgenus Betabacterium. Lysozyme produced by lactic acid bacteria is one of the components in the total complex of the mechanism of biological activity of this important group of microorganisms.

Animals↗
PubMedDate not supplied

[Lactobacilli of freshwater fishes].

Normal microflora in the intestinal tract of fishes inhabiting fresh-water reservoirs includes lactic bacteria. The number of the bacteria depends on the animal species, the composition of food, the age, and the season. The highest number of these microorganisms (hundreds of millions per gram of the intestinal content) is found in carps. Enterococci are most often encountered in fishes inhabiting ponds: Streptococcus faecalis Andrewes a. Horder, Str. faecium Orla-Jensen, Str. bovis Orla-Jensen. Lactobacilli are more typical of fishes in water reserviors: Lactobacillus plantarum (Orla-Jensen) Bergey et al., L. casei (Orla-Jensen) Hansen a. Lessel, L. casei var. casei, L. casei var. rhamnosus, L. Casei var. alactosus, L. leichmannii (Henneberg) Bergey et al., L. acidophillus (Moro) Hansen a. Mocquot, L. Fermenti Beijerinck, L. cellobiosus Rogosa et al., L. Buchneri (Henneberg) Bergey et al. The content of lactic bacteria varies in water reservoirs; their highest content is found in ooze (tens of thousands per gram).

Animals↗