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Biomedical subjects

N Kamitani

Publications and source records attributed to N Kamitani.

28 records · Page 2Linked to original sources
PubMed1984

[Tumorigenicity of the cell lines (TTK-1 cell lines) derived from normal human decidua in nude mice].

Two cell lines, TTK-1(E) and TTK-1(F), derived from normal human early decidual tissue have been maintained in culture through forty subcultures since July, 1979. These cell lines were transplanted subcutaneously into nude mice at 6 weeks of age to investigate their tumorigenicity. Rapidly growing tumor nodules formed at the site of implantation. The incidence of tumor growth was 60% in TTK-1(E) and 80% in TTK-1(F). The tumor tissues were composed of poorly differentiated cells arranged in cord-like and/or gland-like structures, and showed the malignant histological characteristics. Light microscopic and electron microscopic studies revealed that the properties of the tumor cell populations are identical with those of the culture cells of the two cell lines before implantation. These results obviously indicated that the tumors had grown from the implanted cells. Although the tumors developed from the respective two cell line showed some common histological features, they apparently differed in light microscopic and electron microscopic findings. Tumorigenesis with malignant features could be attributed to the in vitro spontaneous neoplastic transformations during successive subcultures, which might be useful as an in vitro model of endometrial carcinogenesis.

Adenocarcinoma↗
PubMed1981

[An ultrastructural study of penetration and replacement process by trophoblast cell into human maternal vessel (author's transl)].

To elucidate the mechanism of trophoblastic penetration into maternal vessels and the function of the trophoblast replacing the endothelium, feto-maternal junctional zone (FMJZ) removed from human uteri in early gestational stages of 4 to 11 weeks were morphologically investigated. The trophoblast cells which can approach to or penetrate the maternal vessels and can replace the endothelial cell were only syncytiotrophoblast cells. Since microfilaments aggregated in bundle in cytoplasm of the syncytiotrophoblast cells which are approaching to the maternal vessel inserted into dense bodies or dense patches, it is considered that the microfilaments are composed of thin filaments mainly like in smooth muscle cell. Degranulation of rER and lysosomal structure or aggregation of microfilaments are occurred in endothelial cells penetrated or closely contacted by field horsetail-like cytoplasmic processes of syncytiotrophoblast cell, respectively. It is possible that the syncytiotrophoblast cell and maternal endothelial cell have similar origin, since both cell can be in close contact with tight junction and simple apposition like intrinsic endothelial junction.

Blood Vessels↗
PubMedDate not supplied

Well-differentiated papillary mesothelioma involving the peritoneal and pleural cavities: successful treatment by local and systemic administration of carboplatin.

Well-differentiated papillary mesothelioma (WDPM) of the peritoneum is a rare form of epithelial mesothelioma. It usually shows an indolent course and no standard treatment is available. Only a few cases of WDPM in the pleural cavity have been reported. We report on a 56-year-old post-menopausal woman who presented with ascites and right pleural effusion. Laparotomy followed by biopsy established the presence of WDPM in the peritoneum and pleural cavity. The patient was successfully treated with local and systemic administration of carboplatin.

Antineoplastic Agents↗
PubMedDate not supplied

Hypersensitivity reaction to carboplatin during treatment for ovarian cancer: successful resolution by replacement with cisplatin.

Development of hypersensitivity reactions to carboplatin (CP) during cancer treatment makes optimal chemotherapy difficult to achieve. Many approaches have previously been used following the development of reactions to CP. We report on a patient with ovarian cancer who developed a hypersensitivity reaction to CP. The patient was successfully treated following replacement of carboplatin with cisplatin.

Aged↗
PubMedDate not supplied

Establishment of a liver metastatic model of human ovarian cancer.

We have established an in vivo experimental model in which human ovarian cancer grows in the ovary of nude mice and metastasizes to parenchymatous organs. An ovarian cancer cell line was orthotopically injected into the nude mouse ovary together with Matrigel by microsurgical techniques. The cells grew locally in the ovary and metastasized to the peritoneum, colon, omentum, liver, and spleen. When the cells were injected into the intraperitoneal cavity with Matrigel, they formed carcinomatous peritonitis but neither ovarian tumor formation nor the metastasis to parenchymatous organs was detected. Taken together, these findings indicate that the microenvironment of the ovary seems to be essential for metastasis of implanted human ovarian cancer cells. This in vivo experimental model allows us to investigate the mechanism of the metastasis of ovarian cancer, it will be also useful for the establishing a new therapeutic approach to preventing metastasis of human ovarian cancer to parenchymatous organs.

Animals↗
PubMedDate not supplied

Establishment of a cell line from a neuroendocrine carcinoma of the endometrium and an invasion model.

A new cell line (YKK) was established from a primary tumor of neuroendocrine carcinoma of the endometrium. Cultured YKK cells are polygonal in shape. The modal chromosome number is 46 XX. The doubling time of the cells, after the 19th passage, is 38 hours and this cell line has been propagated continuously by serial passage over 50 passages for the past 2 years. YKK displays characteristics resembling the original tumor cell from the donor patient: it contains the neuron-specific enolase and chromogranin antibody, produces neuron-specific enolase, and is sensitive to cisplatin, carboplatin, and etoposide. When YKK cells are transplanted subcutaneously together with Matrigel into nude mice they form a very large tumor invading the subcutaneous muscular tissue. This experimental model is useful for studying the mechanism(s) regulating the invasion of this tumor into muscular tissue, and for the establishment of new anti-cancer treatments for neuroendocrine carcinoma.

Animals↗