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Biomedical subjects

N Kanda

Publications and source records attributed to N Kanda.

125 records · Page 7Linked to original sources
PubMed1978

Differential staining in the secondary constriction regions of human chromosomes A1, C9, and E16 by a heat-hypotonic treatment technique.

The secondary constriction (SC) regions of human chromosomes A1, C9, and E16 were preferentially stained with Giemsa when the mitotic cells in a hypotonic solution were heated in a water bath before fixation. The staining intensity of these regions increased from E16 through A1 to C9. The SC region of C9 was stained more intensely as the treatment temperature was raised from 45 degrees C to 60 degrees C, while the staining intensity of the SC region of E16 reached a peak at 50 degrees C and then decreased gradually with increasing temperature. The SC region of A1 showed stable differential staining between 50 degrees C and 60 degrees C. These observations suggest a heterogeneous nature of the SC regions of human chromosomes, probably reflecting a difference in the chromosomal proteins or the DNA-protein interaction in these regions.

Adult↗
PubMed1976

Banding pattern observed in human chromosomes by the modified BSG technique.

A successful modification of the BSG technique to reveal C and R bands simultaneously in human chromosomes is described. Conventional air dried preparations were treated first with 0.1 N HCl for 30 min at room temperature, then denatured in freshly prepared 3% aqueous solution of Ba(OH)2SH2O for 10 min at 50 degrees C. After rinsing, the slides were incubated for 1 h at 60 degrees C in 2 X SSC, and stained with Giemsa. The striking intense staining pattern could be observed in chromosome No. 19. The factors involved in the present technique were analyzed changing the concentrations of the reagents and the treatment time. It was evident that R, T and C bands correspond to a progressive destruction of the chromosome structure mainly by the Ba(OH)2SH2O solution.

Azure Stains↗
PubMed1975

DB-2073, a new alkylresorcinol antibiotic. I. Taxonomy, isolation and characterization.

A new antibiotic, DB-2073, was isolated in crystalline form from the fermented broth of Pseudomonas sp. B-9004. The compound is a alkylresorcinol antibiotic. The antibiotic melts at 86-88 degrees C. The molecular weight of 236 was determined by mass spectroscopy and the molecular formula was calculated as C15H24O2. The antibiotic has antimicrobial activity against Gram-positive bacteria, mycobacteria and fungi.

Anti-Bacterial Agents↗
PubMedDate not supplied

c-erbB-2 and c-erbA-1 (ear-1) gene amplification and c-erbB-2 protein expression in Japanese breast cancers: their relationship to the histology and other disease parameters.

We studied c-erbB-2 and c-erbA-1 (ear-1) gene amplification, and c-erbB-2 protein expression in 123 primary Japanese breast cancers. c-erbB-2 amplification was found in 19 of the 123 tumors (15%), and c-erbA-1 was coamplified in 7 of the 19. The presence or absence of c-erbB-2 amplification correlated with the grade of cellular atypism (P = 0.008), or that of mitotic index (P = 0.002), but not with the histologic types. The tumor size (P = 0.04) and the lymph node status (P = 0.06) were associated, but the patients' age, the TNM stage, or the presence or absence of estrogen or progesterone receptors was not associated, with c-erbB-2 amplification. There were no differences in the histologic type, cellular atypism, mitotic index, and other disease parameters between tumors with c-erbB-2 amplification only and those with coamplification of c-erbB-2 and c-erbA-1. Paraffin sections from all 19 tumors with c-erbB-2 amplification, and those from only one of 104 tumors without the amplification were positively stained with polyclonal anti-c-erbB-2 protein antibody. Since the correlation between the amplification and the protein expression was excellent, such immunohistochemical studies may be substituted for the time-consuming DNA studies using Southern blotting.

Adult↗
PubMedDate not supplied

Polyarthritis, mononeuritis multiplex and eczematous ulcerative skin rash in a patient with myelodysplastic syndrome and peripheral large granular lymphocytosis.

A patient with polyarthritis, peripheral mononeuritis multiplex with spatial and temporal fluctuation, and eczematous, ulcerative skin rash in the lower extremities was found to have myelodysplastic syndrome (MDS) in the bone marrow and concomitant large granular lymphocytosis in the peripheral blood. Histochemical study showed that cells with large granular lymphocyte markers (CD2+, 11b+, 16+, 57+, HLA-DR+) had infiltrated into the skin and around the nerve fibers. Both the bone marrow dyscrasia and rheumatic manifestations of this patient improved significantly after prednisolone therapy. The unusual rheumatologic manifestations of this patient appear to derive from a delicate balance between MDS and large granular lymphocytosis.

Aged↗