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N Kitamoto

Publications and source records attributed to N Kitamoto.

47 records · Page 3Linked to original sources
PubMed1984

Cross-reactivity between cowpox and vaccinia viruses with monoclonal antibodies. Brief report.

Hybridoma cell lines producing monoclonal antibodies against cowpox virus (CPV) and vaccinia virus (VV) were established to examine the specific and cross-reactive antigenic determinants of these viruses. Monoclonal antibodies against CPV (LB red strain) and VV (Lister strain and Ikeda strain) were classified into several groups on the basis of the results of immunofluorescence and haemagglutination inhibition tests. It was suggested that the groups defined above include the group of antibodies reacting with each of known major antigens of poxvirus, i.e. nucleoprotein (NP) antigen, heat labile and stable complex (LS) antigen, haemagglutinin (HA), cell surface antigen (CSA) and type A inclusion body (A).

Antibodies, Monoclonal↗
PubMed1982

Hanganutziu-Deicher type-heterophile antigen-positive cells in human cancer tissues demonstrated by membrane immunofluorescence.

The possible expression of Hanganutziu-Deicher (H-D)-type heterophile antigen on the cell surface of several types of human cancer tissues was investigated by the membrane immunofluorescence test with anti-H-D serum. Anti-H-D serum of high titer was obtained from chickens immunized with H-D antigen-active glycosphingolipid, N-glycolylneuraminyl-lactosylceramide (equine hematoside). H-D antigen was demonstrated in 3 to 6 gastric cancer tissues, 2 of 3 lung cancer tissues, and 2 of 4 breast tissues examined, but not in 5 colorectal cancer tissues examined.

Antigens, Heterophile↗
PubMed1981

Expression of Forssman antigen of avian lymphoblastoid cell lines transformed by Marek's disease virus or avian leukosis virus.

The expression of Forssman-type heterophile antigen on Marek's disease (MD) virus (MDV)-transformed cell lines, MDCC-MSBI 1, -HP1, -RP1 and -BP1, and avian leukosis virus (ALV)-transformed cell lines, LSCC-1104B1 and -1104X5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. Forssman antigen was detected on a high percentage of the cells in two ALV-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. Of the MDV-transformed cell lines tested only the RP1 and BP1 cell lines, derived from transplantable MD tumours, expressed Forssman antigen, while the MSB1 and HP1 cell lines, derived from MD lymphomas, did not. Forssman antigen appears to be unrelated to MD tumour-associated surface antigen (MATSA).

Animals↗
PubMed1981

Latency of herpesvirus of turkey and Marek's disease virus genomes in a chicken T-lymphoblastoid cell line.

The properties of latent herpesvirus of turkey (HVT) and Marek's disease virus (MDV) genomes have been studied in virus-non-producer MDCC-BO1(T) cells, a T-lymphoblastoid cell line derived from spleen cells of an HVT-vaccinated chicken. The numbers of the two virus genomes in BO1(T) cells remained stable at 1.6 to 1.8 HVT genome equivalents/cell and 3.4 to 3.8 MDV genome equivalents/cell throughout a number of passages and were not decreased by the presence of phosphonoacetic acid in the culture. When the culture temperature of the MDV-producer MDCC-MSB1 cell line was shifted from 41 to 37 degrees C, the cells cultured at 37 degrees C contained about five times as many virus genomes as those cultured at 41 degrees C. In contrast, the numbers of the two virus genomes in BO1(T) cells were not increased by culture at 37 degrees C. RNA extracted from BO1(T) whole cells and from the polyribosomal fraction hybridized to both MDV and HVT DNAs, indicating the expression of both latent virus genomes. Digestion of cell nuclei with micrococcal nuclease revealed that both latent HVT and MDV genomes possess a nucleosomal structure. Closed circular MDV DNA was demonstrated in BO1(T) by isopycnic centrifugation of DNA in ethidium-bromide-CsCl gradients.

Animals↗
PubMed1980

Persistence of genomes of both herpesvirus of turkeys and Marek's disease virus in a chicken T-lymphoblastoid cell line.

A cell line tentatively designated as MDCC-BO1(T), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (HVT). BO1(T) cells were T lymphoblastoid cells and the more than 95% of them had Marek's disease (MD) tumor-associated surface antigen (MATSA). However, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-HVT and -MD virus (MDV) sera. The virus could be rescued from BO1(T) cells by co-cultivation with chick embryo fibroblasts (CEF). The DNA of the rescued virus was characterized as HVT DNA by its sedimentation profile in a neutral glycerol gradient and its endonuclease Hind III cleavage-pattern. Ultrastructural studies on CEF infected with the rescued virus revealed the presence of HVT-like virions. However, DNA-DNA reassociation kinetics showed that the BO1(T) cells contained a few copies of NVT and also MDV genomes.

Animals↗
PubMed1980

Demonstration of heterophile antibody in chicken antiserum against Marek's disease tumor-derived cell line, MSB-1.

Sheep red blood cells (SRBC) were agglutinated by all six chicken anti-MSB-1 sera examined, but not by sera of thirty specific pathogen-free chickens. The SRBC-agglutination titer was greatly reduced by absorption with SRBC, bovine red blood cells (BRBC) or guinea-pig kidney cells (GPKC). The dissociation of heterophile antibody and antibody to so-called Marek's disease tumor-associated surface antigen (MATSA) is discussed.

Animals↗
PubMed1980

Distribution of Forssman antigen in chickens.

The distribution of Forssman antigen in various tissues of chickens was studied by the indirect and direct immunofluorescence (IF) tests using rabbit immune serum against purified Forssman glycosphingolipid (GSL) [GalNAc (alpha, 1-3)GalNAc(beta, 1-3)Gal(alpha, 1-4)Gal(beta, 1-4)Glc-ceramide]. A general finding in all tissue sections was the presence of Forssman antigen in vascular endothelium and perivascular connective tissues. Erythrocytes gave a Forssman positive reaction, but the intensity of fluorescence was slight. Forssman antigen was found in about 20% of all peripheral leukocytes. Fractionation of lymphocytes from peripheral blood into T and B lymphocytes showed that about 30% of the B lymphocytes gave a positive reaction, while only 2-3% of the T lymphocytes gave a positive reaction. About 40% of bone marrow cells expressed Forssman antigens. On the other hand, about 40-50% of adherent cells in vitro cultures from spleen were Forssman-positive. The antigen was also abundant in hematopoietic organs, such as spleen, thymus and the bursa of Fabricius. Positive reactions in the digestive tract, ovary, testis and skin varied from portion to portion of the tissues. In brain and spinal cord, only the vascular endothelium gave a positive reaction. In peripheral nerves, only the connective tissue stroma around the nerve fibers gave a positive reaction. In other tissues, the distribution of the antigen was generally in accord with the results of Tanaka and Leduc (1956). These positive reactions in the IF test were specifically inhibited by addition of purified Forssman GSL. The distributions of Forssman antigen in tissues were found to be the same in chickens of various ages.

Animals↗
PubMed1979

Marek's disease virus and herpesvirus of turkey noninfective to chickens, obtained by repeated in vitro passages.

The relation between the passage level of Marek's disease virus, C2 strain, and of herpesvirus of turkey (HVT), O1 strain, in cell culture and the level of the serological response of chickens to these viruses was examined. In both cases the immune response of chickens to these viruses decreased with increase in the number of in vitro passages of virus. Virus was not recovered from chickens inoculated with HVT highly passaged in vitro, which had become a high producer of cell-free virus in vitro, and grew equally well at 37 C and 41 C.

Animals↗
PubMed1979

Demonstration of cells with Marek's disease tumor-associated surface antigen in chicks infected with herpesvirus of turkey, O1 strain.

The presence of Marek's disease tumor-associated surface antigen (MATSA) was demonstrated by the direct and indirect membrane immunofluorescent tests, in chicks inoculated 7-10 days earlier with herpesvirus of turkeys (HVT), O1 strain. In in vitro cultures of spleen lymphocytes and ovaries obtained from these chicks, MATSA-positive cells were also detected after 1-7 days cultivation. A possible mechanism of protection by HVT vaccine against Marek's disease is proposed.

Animals↗
PubMed1979

Cell-mediated cytotoxicity of lymphocytes from chickens inoculated with herpesvirus of turkey against a Marek's disease lymphoma cell line (MSB-1).

Using a new device which increases the sensitivity of detection of specific immune lysis of target cells by labeling them with [35S]-methionine, the in vitro cell-mediated cytotoxic response of spleen lymphocytes and peripheral blood lymphocytes from chickens vaccinated with herpesvirus of turkey (HVT), O1 strain, against MSB-1 line cells was clearly demonstrated. The cytotoxic activity was clearly inhibited by pretreatment of effector lymphocytes with anti-T lymphocyte serum and complement. The activity was greater using T cells purified from spleen lymphocytes and peripheral blood lymphocytes than with the unfractionated cells, indicating that T lymphocytes play the main role in effector activity. Using sera from HVG-vaccinated chickens, no significant cytotoxic effects were detected in the complement-dependent antibody cytotoxicity test against MSB-1 cells. These results suggest that cellular immunity against the surface antigen of Marek's disease (MD) lymphoma cells is mainly related to the preventive mechanism against MD incidence by HVT vaccination.

Animals↗