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Biomedical subjects

N Krishnan

Publications and source records attributed to N Krishnan.

31 records · Page 2Linked to original sources
PubMed1975

A rapid method for the assay of guanylate cyclase.

An extremely rapid and sensitive assay for guanylate cyclase utilizing [alpha-32P]-GTP has been developed. It involves incubation of 5-100 mug of enzyme protein with 1 mM [alpha-32P]-GTP in 40 mM Tris HC1 buffer (pH 7.4) containing 3-3 mM MnSO2, 10 mM theophylline and 1 mM cyclic GMP. The reaction is terminated by addition of EDTA, and [32P]-cyclic GMP formed is isolated by sequential chromatography on Dowex-50-H+ and alumina. Recovery of 75-85% of [3H]-cyclic GMP and a blank of 0.001-0.003% of added [32P]-GTP was routinely obtained. The [32P] radioactivity isolated was shown to be cyclic GMP by a variety of techniques. The assay has also been shown to be applicable for a variety of tissues.

Adrenal Glands↗
PubMedDate not supplied

Effect of the concentration and duration of application of mitomycin C in trabeculectomy.

Ninety-three consecutive trabeculectomies in which mitomycin C (MMC) was applied intraoperatively, either at a concentration of 0.5 mg/mL for 5 minutes (n = 48) or at a concentration of 0.4 mg/mL for 3 minutes (n = 45), were reviewed to determine whether there was a significant difference between the two groups in terms of intraocular pressure (IOP) control and the incidence of complications. Preoperative and postoperative IOPs in the two groups did not differ significantly (P = .817 and .131, respectively). The 5-minute group had a higher incidence of serous choroidal detachments (P = .001). The incidence of postoperative hypotony and filtration failure with uncontrolled IOP in the two groups did not significantly differ (P = .177 and .582, respectively). Thus, intraoperative application of MMC at a concentration of 0.4 mg/mL for 3 minutes controlled postoperative IOP as effectively as MMC at a concentration of 0.5 mg/mL for 5 minutes, with a significantly lower incidence of choroidal detachments.

Adult↗