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Biomedical subjects

N Kuji

Publications and source records attributed to N Kuji.

44 records · Page 3Linked to original sources
PubMed1982

[Pharmacokinetic study of piperacillin in the field of obstetrics and gynecology].

The following trial was conducted as a fundamental study in the application of piperacillin (PIPC) in the field of obstetrics and gynecology. A dose of 1 g was administered to 10 patients by intravenous injection and the concentrations of PIPC in the serum and the various tissues of the uterus were measured. This data was analyzed by the computer. 1. These concentrations were analyzed based on a two- or three-compartment model. We obtained the pharmacokinetic parameters and described the simulation curves. Correspondence was observed between the calculated and the actually determined concentrations. 2. PIPC was rapidly distributed to the tissues of the uterus and exhibited high concentrations. Transfer to these tissues correlated well with serum concentrations.

Adult↗
PubMed1980

Hetero-transplantation of human endometrial carcinoma into nude mouse. Effects of sex steroid hormones on morphological aspects of undifferentiated adenocarcinoma cells.

Seven cases of human endometrial carcinoma were transplanted into nude mice. Two of these cases, histologically classed as undifferentiated adenocarcinoma, were succeeded in the initial transplantation, but only 1, designated JEA-1, was established as serially transplanted tumor line in nude mice, which contained no estrogen and progesterone receptors assayed by sucrose density gradient centrifugation. JEA-1 tumor cells transplanted serially maintained the morphological and the functional characteristics of the original human tumor. Consequently, the JEA-1 tumor line will be useful for the experimental model of cancer treatments and the analysis of sensitivity for sex steroid hormones. Effects of sex steroid hormones on JEA-1 tumor cells had been examined and the electron microscopic observation and in receptor level, alteration of the tumor tissue were found. Ultrastructurally, the tumor cells which had been administrated estradiol and progesterone revealed various morphological changes resembling that of normal endometrial epithelium in the proliferative and the secretory phase, respectively. In addition, in the tumor tissue which had been administrated estradiol, the low, but positive, estrogen receptor sites were detectable. These findings may suggest that means of estradiol exposure will be able to induce the estrogen and progesterone receptors in endometrial carcinoma initially without these hormone receptors.

Adenocarcinoma↗
PubMedDate not supplied

Protein kinase A activity and protein phosphorylation during the mouse sperm acrosomal reaction.

Using two-dimensional gel electrophoresis, changes in protein phosphorylation caused by cyclic nucleotide-dependent protein kinases were analyzed with or without exposure to a protein kinase inhibitor, H-8, during the mouse sperm acrosomal reaction. The acrosomal reaction, induced by the treatment of sperm with dibutyryl cyclic AMP or dibutyryl cyclic GMP, was inhibited by H-8. The activities of cyclic AMP-dependent protein kinase (PKA) and cyclic GMP-dependent protein kinase induced by the sperm extract were also inhibited by H-8. When endogenous PKA in sperm was activated by the addition of cyclic AMP, a 45-kDa protein spot identified by electrophoresis indicated the occurrence of phosphorylation in vivo. Furthermore, the enhanced phosphorylation of the 45-kDa protein spot was inhibited by H-8. These results suggest that the PKA-catalyzed phosphorylation of the 45-kDa protein may be involved in the regulation of the mouse sperm acrosomal reaction.

Acrosome Reaction↗
PubMedDate not supplied

Apico-aortic conduit bypass operation in a patient with calcified ascending aorta due to Werner's syndrome.

A 53-year-old Japanese man with Werner's syndrome underwent successful apico-aortic conduit bypass operation. Preoperative examination showed severe calcification in the aortic root and ascending aorta. A composite graft was implanted from the left ventricular apex to the descending thoracic aorta with femoro-femoral bypass. To our knowledge, this case is the first surgical correction of a cardiac lesion in a patient with Werner's syndrome.

Aorta, Thoracic↗