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Biomedical subjects

N Kuljić-Kapulica

Publications and source records attributed to N Kuljić-Kapulica.

At least 19 recordsLinked to original sources
PubMed1999

Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients.

Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33 degrees C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis.

Animals↗
PubMedDate not supplied

[Detection of human papillomaviruses in cervical intraepithelial neoplasms].

Over the period 1994-1998 1000 patients suffering from intraepithelial cervical lesions (SIL) were examined for the presence of Human Papilloma Virus (HPV) infection. Today it is completely acknowledged that certain types of these viruses play a key role in the onset and evolution of these diseases. The study showed that SIL of the cervix appears in very young women. The highest incidence was found in age groups 24-28 years (21.10%) and 29-33 years (19.10%). Thus, over 40% of the entire study group patients was under 34 years of age. Histological analysis of tissue samples showed that 79.30% of patients had lesions of low histological grade (LSIL), while in 20.70% of them the disease progressed to a high histological level (HSIL). Detection of Human Papilloma Virus Infection revealed negative results in 34.90% of patients, 17.80% were 6/11 positive (non-oncogenic type), and in 47.30% oncogenic types were found (16, 18, 31, 33). This finding in the LSIL group was exceptionally important since it influenced the approach to treatment. Oncogenic types of viruses were detected in 34.30% of LSIL lesions; it indicates a high progression potential of pathological changes.

Adolescent↗
PubMedDate not supplied

[Effect of certain aspects of female sexual behavior on the development of cervical intraepithelial neoplasia].

The paper presents results of investigation of certain parameters of female sexual activity and their effect on the appearance of squamous intraepithelial cervical lesions (SIL). This correlation was investigated in the context of Human Papilloma Virus (HPV) infection, in view of the role attributed to certain types of these viruses in the appearance and development of cervical SIL. Research was done on 1000 patients suffering from low histological grade (LSIL) or high histological grade (HSIL) intraepithelial cervical neoplasms. It transpired that such lesions were most frequent in women in the second, third and fourth decade of life, and that one third of pathological lesions was a function of age. Human Papilloma Virus infection was detected in 65% of patients. The frequency of HPV negative results or presence of non-oncogenic types of viruses (6 and 11) was significantly higher in LSIL type lesions, while the frequency of detection of oncogenous HPV types (16, 18, 31 and 33) was significantly higher in more severe stadiums of the disease. Of the investigated parameters of sexual activity, early onset of such activity and several sexual partners proved to be significant. The number of parturitions and abortions did not appear to be significant risk factors for the onset and development of these diseases.

Adolescent↗
PubMedDate not supplied

Locally produced specific cytomegalovirus immunoglobulin for intramuscular use as a therapy in renal allograft recipients.

Immunomodulator, i.e. specific hyperimmune anticytomegalovirus immunoglobulin for intramuscular administration, produced in 1999 with the aim of prevention of CMVI, and the development of the disease, was for the first time applied in kidney transplant recipients in January 2000, in the Center for kidney transplantation at the Military Medical Academy. Therapy was administered in four cytomegalovirus (CMV)--seronegative kidney recipients from CMV-seropositive donors--the combination that in the majority of cases lead to the development of CMVI/disease resulting in transplant rejection. Patients received 0.2-0.3 ml/kg of cytomegalovirus immunoglobulin (CMVIG) 6 hours before the transplantation, and subsequently the same dose during the following 5 weeks. Simultaneously, they received ganciclovir in therapeutic doses adjusted according to creatine clearance during the first three post-transplantation months (2 weeks parenterally, the rest orally). Kidney transplant recipients tolerated well i.m. applied CMVIG without any adverse effects. Test result obtained from the Paul-Erlich Institute, Germany in 1999 spoke in favor of the quality of the first national CMVIG preparation.

Antibodies, Viral↗
PubMedDate not supplied

[Coronaviruses].

Corona viruses belong to a group of not yet well known viruses isolated in patients with infections of the upper respiratory organs, especially in winter months. It is presumed that they provoke about 15% - 20% of all infections. However, in the last years more attention has been paid to the role of human corona viruses in the provocation of sickness in man and they are becoming a special field of interest in scientific studies.

Antibodies, Viral↗
PubMedDate not supplied

[Importance and diagnosis of Epstein-Barr virus infection].

The 103 serum samples have been examined for the presence of the specific antibodies against Epstein-Barr virus (EBV): IgG and IgM antibodies to viral capsid antigen (VCA), antibodies to nuclear antigen (EBNA) and antibodies to early antigen (EA). Sixty nine studied sera belonged to patients with suspected infective mononucleosis (IM) while other sera were sent with the diagnosis which could be associated with EBV infections. The results obtained have suggested the necessity of examinations of EBV markers in all states suspected of EBV infection.

Antibodies, Viral↗
PubMedDate not supplied

[The significance of determination of streptococcal deoxyribonuclease B antibody titers].

Results obtained by testing 266 sera using antistreptolysin O (ASTO) and antideoxyribonuclease B (ADNB) tests were compared. Upper limit of the normal ADNB titer was determined for population by analysing titer levels in 258 samples taken from healthy subjects (blood donors) and it was 225 IU/ml. Increased titer of one or both antibodies was found in 76 (29%) sera-ASTO and (or ADNB-positive sera being the much larger number than the positive sera number obtained by ASTO test) 40-15% 7. This confirms necessity of the ADNS test application in all patients with normal ASTO titer.

Antibodies, Bacterial↗
PubMedDate not supplied

[Neutralizing antibodies to Coxsackie virus group B 1986-1988].

Presence of neutralizing antibodies to Coxsackie B viruses (CBV) 1-5 were examined in 2885 human sera by the neutralization test. The most frequently detected antibodies were to B4 viruses (in 1495 sera--51.82%) and to B3 (1371--47.52%). Antibodies to other serotypes were found in lower percentage: to B1 in 387 samples (13-41%), to B2 in 404 sera (14%) and in B5 in 332 sera (11.5%). Presence of neutralizing antibodies to human CBV in heart diseases were also analysed in 67 serum pairs. In this group of examinees the antibody titer dynamics was found in 41.79% sera.

Antibodies, Viral↗
PubMedDate not supplied

[Analysis of herpes simplex virus DNA using restriction endonucleases].

DNA of herpes simplex virus was analysed by restriction endonucleases EcoRI, BgII and HindIII. By this method it is possible to differentiate type 1 and type 2 of viruses. DNA of herpes simplex virus was prepared according to the method of Kinnunen and his collaborators which is described in detail.

DNA Restriction Enzymes↗
PubMedDate not supplied

[Detection of antibodies to cytomegaloviruses, herpes simplex viruses and varicella-zoster viruses in persons positive for human immunodeficiency virus antibodies].

Forty-seven anti-HIV positive sera and 47 sera of normal individuals were analyzed to the presence of IgG and IgM antibodies versus CMV, HSV and VZ. IgG antibodies in comparison to CMV were found in 27 cases (57.44%); in comparison to HSV in 40 (85.1%) subject and to VZ in 33 anti-HIV positive subjects. The mean absorbance value was higher in anti-HIV positive cases than in controls. IgM antibodies in comparison to CMV were found in 3, and in comparison to HSV in 2 anti-HIV positive sera. No IgM antibodies were found in the control group.

Antibodies, Viral↗
PubMedDate not supplied

[Viral hepatitis C. Personal experience with diagnostic, monitoring and epidemiologic studies].

In the period from 1991 to 1993, 18 patients with the acute viral hepatitis type C were treated and followed up--13 men and 5 women, the average age of 42 years. The group of 6 patients (4 men and two women) with the "old" proven liver cirrhosis, whose etiology was unknown for 20 years, was analyzed. The investigations showed that the acute viral hepatitis type C usually began with the mild subjective disorders. The half of the patients did not have either jaundice or hepatomegaly, only half of them recover, and the first cases of cirrhosis could have been expected almost as early as a year after the beginning of illness. The developed liver cirrhoses remained compensated for years. About 58% of patients got infected parenterally, and the rest of them could have gotten infected in other manner. ELISA test showed great reliability in proving the antibodies against the viral hepatitis type C in the confirming the diagnosis of illness.

Acute Disease↗
PubMedDate not supplied

[Clinical manifestations of infectious erythema in adults].

The infectious erythema is usually childhood disease associated with rash and caused by parvovirus B19. At the beginning of 1993., at the Clinic for infectious and tropical diseases of the Military Medical Academy 36 patients were treated for this disease, the average age 21 years. The prodromal phase of illness lasted approximately 2.9 days, the elevated temperature 5.2 days, and 17 patients had temperature above 39 degrees C. The rash was usually maculo-papullous and lasted about 6 days. Four patients had the recurrence of rash. The polyarthralgia appeared for short in six patients and in one patient lasted longer than 4 months. The mild anemia during infectious erythema was observed in 18 patients. IgM antiparvo B19 antibodies were proven in serum in 91.7%, and IgG antibodies in 58.3% of patients.

Adolescent↗
PubMedDate not supplied

[Chlamydia trachomatis and acute pelvic inflammatory disease].

The incidence of Chlamydia trachomatis infection in women with acute pelvic inflammatory disease has been studied. Chlamydia trachomatis was isolated in 20 (35.71%) of 56 examined women. In all women with positive isolation, the infection was also confirmed serologically.

Acute Disease↗