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Biomedical subjects

N L Mel'nikova

Publications and source records attributed to N L Mel'nikova.

10 recordsLinked to original sources
PubMedDate not supplied

[Health status of disabled preschool children and determining factors].

Children's invalidism is a serious social, moral and financial problem in Russia. Approaches to its solving are not fully formulated now and need further study. The paper reports the results of the original trial of social and health status, life style of invalids of preschool age. The system of measures is proposed to improve rehabilitation of handicapped children.

Adolescent↗
PubMedDate not supplied

[Immunological characteristics of LPV oncornavirus produced by T-9 human cell line].

The results of immunological study of oncornavirus (LPV strain) produced by a continuous human T-9 cell line are presented. The method of immunodiffusion in agar revealed the following constituent antigens in the virus: (a) those identical to internal and external antigens of Mason-Pfizer oncornaviruses particles; (b) those specific only for LPV virus and not common in other known oncornaviruses of mammals. These antigens were common in LPV virus from T-9 culture and from xenogeneic T-BHK culture in which Mason-Pfizer virus antigens were absent completely; and (c) interspecific antigen of oncornavirus C type present in minimal amounts. Localization of the above antigens in virus particles of LPV oncornavirus was confirmed by the electron immunoenzyme method. Upon separation of virus polypeptides in polyacrylamide gel it was found that the antigens cross-reacting with Mason-Pfizer virus as well as LPV specific antigens were localized both in the main inner virus protein and in the surface glycoprotein.

Antigens, Viral↗
PubMedDate not supplied

[Characteristics of Namalwa cells--a substrate for production of human interferon].

The properties of lymphoid Namalwa cell line propagated at the USSR Academy of Medical Sciences Research Institute of Viral Preparations for interferon production are described. The scanning and transmissive electron microscopy studies of the cells showed their morphological stability and the absence of microbial contamination. The 46-48-chromosome cells comprised 85% of the population, hypodiploid cells (44-45 chromosomes), 9%, tetraploid and hypertetraploid cells, 3%. Spontaneous aberrations were detected in 3% of the chromosome. Inoculation of the cells into unsuppressed laboratory animals (rabbits, guinea pigs, adult or suckling mice) or chick embryos did not cause the development of any pathological process. Namalwa cells were shown to produce interferon after multiple (up to 4 times) induction with Newcastle disease virus.

Burkitt Lymphoma↗
PubMedDate not supplied

[Isolation of purified and concentrated human interferon from Namalwa cells].

The interferon produced in the cultured Namalwa cells was purified and concentrated according to the method of K. Cantell and S. Hirvonen developed for purification of leukocyte interferon. A preparation concentrated 500-fold had the antiviral activity of 10(6) IU/ml at a specific activity of 1-3 X 10(6) per 1 mg of protein. The amount of protein in the preparation was approximately 0.6 mg/ml, that of cell DNA less than 100 ng/ml. The experiments demonstrated that the virus inducer of interferon and possible contaminant viruses were removed on purification. The preparation had no oncogenic potency on inoculation of newborn Syrian hamsters, was harmless by intraperitoneal inoculation of mice and on multiple inoculations on rabbit eye cornea, was not toxic in cell cultures.

Animals↗
PubMedDate not supplied

[The antiviral action of recombinant forms of the human T-lymphocyte CD4 receptor].

A recombinant protein containing the first 179 N-terminus amino acids of human T-lymphocyte CD4-receptor was synthesized in E. coli cells. This recombinant protein was shown to interact with OKT4A and Leu3a monoclonal antibodies competing with HIV gp120 glycoprotein for binding with the native CD4 receptor. Experiments in vitro in human T-lymphocyte cultures showed that the recombinant CD4-protein in concentrations of 1 to 10 micrograms/ml inhibited the virus-induced syncytium formation, HIV replication in cell culture, synthesis of HIV reverse transcriptase and other virus-specific proteins, that is, behaved as a HIV inhibitor.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Study of human diploid cell strains for the presence of oncornavirus determinants].

The WI-38 strain of human diploid cells (HDC) and two strains, L-58 and L-63 isolated in Moscow Research Institute of Virus Preparations were examined in the course of sequential passages for the presence of primate oncornaviruses and their antigens. In parallel, the tumorigenic capacity of the cells was tested by inoculating them into the cheek pouch tissue of Syrian hamsters. In all cases the examined HDC cultures contained no oncornavirus particles or their antigens and in no case showed any infectious activity determined in the KC-test. Only on one subculture of the L-63 strain cells at the 44th passage was a positive result obtained in immunoprecipitation test with antiserum to the LPV oncornavirus. Cells of the L-58 strain which had undergone 27 and 43 passages showed tumorigenicity in hamster experiments. In subsequent passages of these HDC strains no such properties were found. The regular control for the absence of oncogenic message is a necessary condition for the use of HDC strains in virological practice.

Cell Line↗
PubMedDate not supplied

[The isolation and characteristics of monoclonal antibodies to recombinant proteins of HIV-1 and HIV-2--the env and gag gene products].

Ten hybridomas secreting monoclonal antibodies (Mab) against recombinant HIV-1 and HIV-2 antigens were produced (3 Mab anti-gag protein, 2 anti-env1, and 5 anti-env2). In the immunoblotting assay all the anti-gag Mabs reacted with HIV capsid protein p24, whereas one of them reacted also with p55 protein and with 7 other polypeptides. Another anti-gag Mab cross-reacted with the antigen of subpopulation of human peripheral blood lymphocytes. The third one interacted with the antigen of both HIV-1 and HIV-2. All the 10 Mabs interacted with natural HIV antigens and can be used for identification and differentiation of HIV-1 and HIV-2.

Animals↗