Transcription of both DNA strands of vaccinia virus genome in vivo.
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Animals↗
Biomedical subjects
N L Varich
Publications and source records attributed to N L Varich.
Newcastle disease virus-specific RNA: an analysis of 24 S and 35 S RNA transcripts.
The denaturation of Newcastle disease virus-specific 24 S and 35 S RNA by heat or formamide treatment led to a shift of a large part (60--80%) of RNA into the 18 S zone. The remaining 20--40% could not be dissociated further by repeated denaturation or by centrifugation in dimethyl sulfoxide-sucrose gradient. Hybridization-competition analysis revealed that the majority (approximately 75%) of the non-dissociable 35 S RNA and almost all the material present in the non-dissociable 24 S RNA were represented by nucleotide sequences homologous to 18 S RNA. On the other hand, the non-dissociable 35 S RNA lacked some of the sequences present in 18 S RNA, since no more than 45% of the labelled 18 S RNA could be displaced from the hybrid by an excess of unlabelled non-dissociable 35 S RNA. The possible origin of 24 S and 35 S RNA is discussed.
Base Sequence↗
Newcastle disease virus-specific RNA: hybridization-competition of the non-dissociable 35 S RNA with individual 18 S RNA species.
The non-dissociable fraction of Newcastle disease virus- (NDV-Y specific 35 S RNA competed efficiently with two individual 18 S RNA transcripts in hybridization-competition experiments with 50 S virion RNA. The third 18 S component could not be displaced from the hybrid by an excess of non-dissociable 35 S RNA.
Base Sequence↗
A quantitative estimation of poxvirus genome fraction transcribed as "early" and "late" mRNA.
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Animals↗
[Utilization of poly U-cellulose and poly U-sepharose for the study of virus-specific RNA of Newcastle disease virus].
Comparative characteristics of poly(U)-cellulose and poly(U)-sepharose used for the study of virus-specific 18S RNA of Newcastle disease virus are presented. Upon chromatography of 18S NDV RNA on any of these columns, approximately 60-70% RNA was adsorbed. The sorbing RNA contained in poly(A) 10-12% of the total amount of adenosine, in contrast to RNA not adsorbed on the column in which poly(A) contained 1-2% of the total amount of adenosine in RNA molecule. The analysis in sucrose density gradient and polyacryl amide gel of the RNAs eluated from the columns showed RNA chromatography under the conditions used not to cause its degradation. The advantages and short-comings of the sorbents used in studies of virus-specific RNA are discussed.
Cellulose↗
[RNA transcripts of both strands of viral DNA in cells infected with vaccinia virus].
Denaturated 3H-thymidine-labeled vaccinia virus DNA was hybridized with an excess of "late" virus-specific RNA isolated from virus-infected chick embryo cell cultures 8 hours postinfection. The percentage of DNA converted into DNA-RNA hybrid under these conditions never exceeded 50%. If the RNA preparation had been self-annealed prior to hybridization, the percentage was decreased slightly. On the other hand, if the self-annealed RNA had been treated with RN-ase and the double-stranded DNA-RNA hybrids had been denaturated, they became capable of converting into hybrids at least 68% of the labeled DNA. These data indicate that transcription of both DNA strands occurs in a large portion (over 68%) of vaccinia virus genome.
Animals↗
[Characteristics of 24S and 35S virus-specific RNA in cells infected with Newcastle disease virus].
The properties of 24S and 35S virus-specific RNAs of Newcastle disease virus were studied after denaturation. It was shown that 60-80% of 24S and 35SRNA consisted of agglomerates of molecules of virus-specific 18S RNA. Undissociable upon denaturation, 24S RNA contained mainly the same components as undenaturated RNA and consisted completely of sequences of virus-specifis 18S RNA. Undissociable 35S RNA contained no "heavy" heterogenous material present in the intact undenaturated RNA and 75% of it consisted os sequences of 18S virus-specific RNA. Some sequences present in undissociable 24S RNA were absent in undissociable 35S RNA. The aggregation of virus-specific na-transcripts and subsequent formation of covalent bonds between transcripts as well as possible formation of "solid" transcripts of the adjacent genes are discussed.
Newcastle disease virus↗
[Virus-specific poli(A)-containing RNA-transcripts in cells infected with Newcastle disease virus].
The techniques of chromatography on poly (U)-cellulose and sedimentation and electrophoresis analysis were used to study poly(A)-containing virus-specific RNA of Newcastle disease virus. The poly(A)-containing molecules were shown to be present in approximately similar amounts (60-80%) in 18S, 24S, and 35S RNA. No poly(A)-containing molecules capable of interaction with poly(U) sepharose were found in 50S RNA. In 18S RNA there are 6 individual poly(A)-containing RNA-transcripts with molecular weights well correlating with those of virus proteins. The average size of poly(A) for 18S and 24S RNA is 60-70 nucleotide residues. In the sedimentation analysis of poly; A)-containing 24S and 35S RNA eluted from poly(U)-columns, some of the material sedimented in the zone of 18S RNA. The presence of complexes of two or several molecules with the sedimentation coefficient 18S in these RNA's is suggested.
Base Sequence↗