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N López Moratalla

Publications and source records attributed to N López Moratalla.

5 recordsLinked to original sources
PubMed2004

In vivo blockade of pemphigus vulgaris acantholysis by inhibition of intracellular signal transduction cascades.

BACKGROUND: Pemphigus vulgaris (PV) is an autoimmune disease characterized by mucocutaneous intraepithelial blisters and pathogenic autoantibodies against desmoglein 3. The mechanism of blister formation in pemphigus has not been defined; however, in vitro data suggest a role for activation of intracellular signalling cascades. OBJECTIVES: To investigate the contribution of these signalling pathways to the mechanism of PV IgG-induced acantholysis in vivo. METHODS: We used the passive transfer mouse model. Mice were injected with IgG fractions of sera from a patient with PV, with or without pretreatment with inhibitors of proteins that mediate intracellular signalling cascades. RESULTS: Inhibitors of tyrosine kinases, phospholipase C, calmodulin and the serine/threonine kinase protein kinase C prevented PV IgG-induced acantholysis in vivo. CONCLUSIONS: These observations strongly support the role of intracellular signalling cascades in the molecular mechanism of PV IgG-induced acantholysis.

Acantholysis↗
PubMedDate not supplied

[Human pluripotent stems cells I].

In the last few years, the great progress of certain fields, such as molecular biology and development, has allowed a detailed knowledge of mechanisms implicated in cellular programing. This has permitted a rapid and unexpected advance in therapeutic cellular strategies. Thus, it has been possible to discover mechanisms involved in cellular differentiation and therefore has opened possibilities for human cellular manipulation and function replacement of damaged cells. Embryonic stem cells, have been obtained from the embryoblast. A lot of types of cellular lineages that include neurons, glial cells, pancreatic islets cells, hepatic cells, osteoblast and adipocytes, have been derived from mouse embryonic stem cells. In the same way, cellular lineages have been obtained by nuclear transference techniques capable of generating embryonic clones. Some scientist intend to evade by this approach, the bioethic reproval for human cloning, emphasizing that this is a "therapeutic cloning". In the present work, we propose to analyze mechanisms that permit stem cells to be pluripotential and discuss the ethical use of embryos as a source for stem cells with therapeutic potential.

Animals↗