Selective staining reactions of involved blood cells prior to symptoms of lymphatic leukemia in mice.
After inoculation with lymphatic leukemia virus, blood smears were obtained daily from young Balb/c mice. When the smears were stained with silver nitrate, a cytoplasmic granular deposition was first observed in the circulating polymorphs with a subsequent abrupt disappearance of any staining reaction in the cytoplasm. No further cytoplasmic reaction was noted in these cells. The mononuclear cells demonstrated a gradually increasing intracytoplasmic concentration of granules. Prior to the appearance of leukemic symptoms, the lymphoid cells showed extensive vacuolization. It is suggested that this staining procedure affords a means for identifying cells undergoing malignant transformation before symptoms are discernible in the host.
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