[Alteration of amino acid concentrations in plasma of ACD-blood during storage (author's transl)].
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Adult↗
Biomedical subjects
N Liappis
Publications and source records attributed to N Liappis.
Quantitative study of free amino acids in human eccrine sweat excreted from the forearms of healthy trained and untrained men during exercise.
The free amino acids in eccrine sweat collected from the forearms of 20 healthy trained and 20 healthy untrained men during controlled exercise were determined quantitatively using ion exchange column chromatography. Sweat was deproteinized by adding an equal volume of 5% sulphosalicylic acid. The amino acid concentrations showed a constant qualitative pattern in sweat and large individual differences. Essential amino acids, such as isoleucine, leucine, lysine, methionine, phenylalanine, and valine were excreted in relatively small amounts. As compared to the trained men, untrained men showed statistically significantly higher concentrations in sweat for the following amino acids: Alanine, arginine, glycine, histidine, isoleucine, leucine, lysine, ornithine, phenylalanine, serine, taurine, threonine, tyrosine, and valine. No significant differences were found for citrulline, cystine, ethanolamine, and methionine. The comparison of the amino acid excretions in sweat obtained under controlled exercise and in urine showed that the amounts of amino acids excreted in sweat under controlled exercise were comparable to the losses of amino acids in urine.
Adult↗
[Non-invasive determination of oxygen saturation with the oxygenmet pulse wave oximeter on fingers, metacarpus and wrist of infants. Comparison with the oxygen saturation calculated from the pH and pO2 of the blood gas analysis (author's transl)].
Oxygen saturation was determined non-invasively with the oxygenmet pulse wave oximeter 1471 and mathematically from the pH and pO2 of the blood gas analysis. The determination of the oxygen saturation by the aid of the oximetric method was carried out on fingers, metacarpus and wrist of 80 infants (male = 44, female = 36 - ages: 1 day - 1 year and 8 months). Blood gas analysis was done just behind the oximetric analysis and oxygen saturation was calculated from the pH and pO2. The comparison of the oxygen saturations determined non-invasively on fingers, metacarpus and wrist among themselves, showed no statistical significant differences. Also no statistical significant differences were observed between the results obtained with the pulse wave oximeter and the results calculated from the blood gas analysis. The comparison of the oxygen saturations determined with the oxygenmet oximeter (mean of fingers, metacarpus, wrist) with the oxygen saturations calculated from the blood gas analysis showed a very close correlation. The correlation coefficient was 0.930.
Blood Gas Analysis↗
[Effect of 17-alpha-hydroxyprogesteronecapronate on sodium excretion and the renin-angiotensin-aldosterone-system in humans].
In seven healthy male subjects, a natriuretic effect of 17 alpha-hydroxyprogesterone caproate (17 alpha-OHPC) was demonstrated. Three of these subjects were kept on an uncontrolled diet and were examined over a period of 12 days. To the remaining four subjects, a single dose of 250 mg 17 alpha-OHPC was given intramuscularly after four days of intake of a chemically defined diet (Vivasorb). In this second test procedure, blood samples were taken in the recumbent position every two hours throughout a period of 12 h after the injection. For two more days and during the days before the administration of 17 alpha-OHPC, blood was taken at 8 a.m. before getting up from bed in same intervals, urine was collected for analysis of sodium and potassium excretion. During the first 12 h after the injection of 17 alpha-OHPC, the urinary sodium/potassium ratio significantly increased in all subjects. Plasma renin activity showed no characteristic changes at this time, whereas the plasma concentrations of aldosterone and cortisol decreased. The decrease of cortisol concentration started immediately after the injection and was more pronounced than that of plasma aldosterone. During the following 36 h, renin activity as well as aldosterone and cortisol concentrations in plasma showed an increase; in contrast, the sodium/potassium ratio decreased. On the basis of these results, the following effects of 17 alpha-OHPC are discussed: (1) an acute natriuresis which may be due to a competitive inhibition of aldosterone at the renal tubules, and (2) an inhibition of pituitary ACTH secretion or of adrenal steroid biosynthesis.
Adult↗
[Study on the determination of creatinine with the beckman creatinine analyzer 2. Comparison with the Jaffé method in protein-free solution (author's transl)].
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Creatinine↗
[gamma-Glutamyl transpeptidase in human eccrine sweat (author's transl)].
UNLABELLED: The concentration of gamma-glutamyl transpeptidase was determined in eccrine, thermal sweat of 56 healthy men (ages 20--60 years) and 48 healthy women (ages 17--55 years). Samples of sweat were collected from the chest and back. RESULTS: The concentrations of gamma-glutamyl transpeptidase showed great individual variations. The sex specific comparison of the concentrations of gamma-glutamyl transpeptidase revealed that the women excreted in the sweat from the chest and the back nearly the double amount of this enzyme. The gamma-glutamyl transpeptidase concentrations determined in the sweat from the chest of the examined men and women were three times higher as compared with those excreted simultaneous from the back of the same persons. In the group of the women was this differences statistically significant.
Adult↗
[Refractometric measurement of total serum protein, comparison of refractometry and biuret test (author's transl)].
Study on the Refractometric Determination of Total Protein in Serum, Comparison of the Refractometric and Biuret Method. Total protein concentration in serum was determined by the aid of the Abbé-refractometer and the biuret method. Both methods showed a good precision and accuracy. The investigation was carried out in 241 sera with normal bilirubin (up to 1 mg/100 ml), cholesterol (up to 200 mg/100 ml) and urea (up to 23,0 mg/100 ml) concentration, in 43 sera with increased (10,6-26,6 mg/100 ml) bilirubin concentration, in 129 sera with increased (200-520 mg/100 ml) cholesterol concentration and in 43 sera with increased (23,0-155,3 mg/100 ml) urea concentration. The comparison of the refractometric values with the values obtained by the biuret method in the 241 sera with normal bilirubin, cholesterol and urea concentration (correlation coefficient = 0,971) showed a close correlation and in the 43 sera with increased bilirubin concentration (correlation coefficient = 0,958) an acceptable correlation. However no close correlations were observed in the 129 sera with increased cholesterol concentration and in the 43 sera with increased urea concentration. The correlation lines diverged proportional with the increase of cholesterol and urea concentration from the expected correlation lines.
Bilirubin↗
[Normal range of free amino acids in cerebrospinal fluid of children (author's transl)].
UNLABELLED: Free amino acids were determined quantitatively in cerebrospinal fluid of 80 boys and 69 girls (ages 1 month-14years). On the basis of the cliniclal criterions these children were considered to be ""healthy''. The total number of examined children was 2000. Cerebrospinal fluid was mixed with an equal volume of 5% sulphosalicylic acid and the amino acids were separated by ion exchange column chromatography. RESULTS: 1. The sexspecific investigation of the 1 month -2 years old children showed that the boys had a statistical significant hgher concentration of lysine and that no statistical significant differences existed between the groups of 2-14 years old children. 2. The age specific comparison revealed that the amino acid concentrations ofaspartic acid, citrulline, histidine, lysine, serine, threonine and tyrosine were in the cerebrospinal fluid of the younger boys statistical significant higher as compared to the older boys and that the concentration of serine in the cerebrospinal fluid of the younger girls was statistical significant higher than in the group of older girls.
Adolescent↗
[Sex-specific differences in free, inorganic sulfate excretion in 24 hours urine of healthy children (author's transl)].
UNLABELLED: Free, inorganic sulfate was determined quantitatively in the 24 h urine of 65 boys and 53 girls (age 6--14 years). The determination of sulfate was carried out by the aid of the spectrophotometric method with barium chloranilate. RESULTS: The following values were obtained for the boys and girls: Boys (ages 6--10 years) 17,66 +/- 11,70 mmol/24 h, girls (6--10 years) 10,70 +/- 10,26 mmol/24 h, boys (10--14 years) 17,40 +/- 12,32 mmol/24 h, girls (10-14 years) 7,26 +/- 8,90 mmol/24 h. No statistical age-specific differences were observed, but the sex-specific investigation revealed that the younger and older boys had a statistical higher excretion of sulfate in 24 h urine as compared to the younger and older girls.
Adolescent↗
[The influence of cortisol treatment on the development of diabetic cataracts in rats (author's transl)].
The influence of daily subcutaneous cortisol injections (10 mg/kg) on the development of cataracts in streptozotocine (STR) diabetes was studied in 6 groups of Wistar rats (n = 10). Cataracts occurred only in the two groups subjected to high STR doses (60 mg/kg). We were astonished to find that in cortisol-treated diabetic animals there were lower blood glucose levels, fewer cases of glucosuria, and less pronounced cataracts than in rats treated with STR only. As STR had been administered after the start of cortisol therapy, it is assumed that the steroid had caused a protection of pancreatic B cells against STR and the onset of a less severe diabetes.
Animals↗