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N M Alykov

Publications and source records attributed to N M Alykov.

10 recordsLinked to original sources
PubMed1980

[Photometric determination of monomycin in biological materials].

A photometric procedure for determination of monomycin in biological materials, such as blood, milk, urine was developed on the basis of the investigation of the reaction of monomycin complex formation with phthalexon-S and praseodymium in water-ethanol medium (1 : 1). The error of estimation of 0.1 microgram of monomycin in 1 ml of a biological material did not exceed 5 per cent (rel.).

Animals↗
PubMed1980

[Rubomycin complexing with copper (II)].

Complexing of rubomycin (H2R) with copper ions (II) in aqueous solutions was studied on the basis of fluorimetric and photometric measurements. H2R interacted with Cu (OH)+ to form the complex CuR with the molar extinction coefficient Ek = 4 . 96 . 10(3) and the stability 1g beta k = 5.54 +/- 0.09. The method of determining H2R in biological materials has been developed. It is based on the ability of the copper entering the complex to extinguish fluorexon luminescence in aqueous solutions.

Animals↗
PubMed1980

[Fluorometric extraction determination of olivomycin].

An extraction fluorometric method for determination of olivomycin in biological materials was developed as a result of the study on the antibiotic complex formation with the gallium ions in aqueous solutions. The method is based on measurement of the fluorescence of a benzene extract of chlorgallate ionic associate with rodamine-6Zh.

Drug Interactions↗
PubMed1980

[Fluorimetric study of methacycline complex formation with beryllium, aluminum, magnesium, calcium and zinc ions].

Complex formation of metacycline (Pv) with beryllium, aluminium, magnesium, calcium and zinc ions in aqueous solutions was studied fluorometrically. The antibiotic formed the following compounds: CaPv2 (pH 9.45), MgPv (pH 10.40), Be Pv (pH 7.0 and 11), Ca Pv (pH 13.5), Zn Pv (pH 7.15) and Al Pv (pH 6.0). Relationship between the solution fluorescence level and the values of pH, the quantum yield of fluorescence of Pv and the complexes and the kinetics of the reactions of complex formation of Pv with Be and Al were studied. The mechanism of Pv complex formation with the metal ions is discussed. A method for analysis of microgram amounts of Pv in biological fluids was developed. The method is based on the antibiotic fluorescent reaction with magnesium ions.

Aluminum↗
PubMed1980

[Spectrophotometric method of determining aminoglycoside antibiotics].

Azo-derivatives of chromotropic acid and azo-dyes of the stilbene series were studies as possible reagents for photometric determination of aminoglycoside antibiotics (AB) in biological materials. The complex of Pr3+ with stilbazochrome-P (Sch-P) proved to be the best precipitant of AB. P (Sch-P) with Pr3+ and AB in acetate and citrate buffer-solutions formed a precipitate with AB:Pr:Sch-P = 1 : 1 : 3. Most of the metallic cations and anions did not prevent formation of the precipitate in the citrate buffer. A procedure for determination of 0.01--100 micrograms of AB in biological materials was developed with the use of Sch-P. The procedure is based on separation of the AB:Pr:Sch-P precipitate and photometry of the sypernatant solution at 560 nm.

Aminoglycosides↗
PubMed1976

[Interaction of morphocycline with beryllium ions].

Behaviour of morphocycline (H5R) and its complex with beryllium ions in acqueous solutions was studied fluorimetrically. The ionization constants of H5R were estimated at pH 1.5-15 according to the data of fluorimetric determination with respect to OH-group: pK1 6.40, pK2 8.25, pK3 10.65, pK4 11.30. Two constants characterizing the deprotonization process with respect to the carbonylic group and nitrogen were also estimated: pK01--1.0 (greater than C = 0) and pK02 4.75 (--N=). Formation of an intensively fluorescing compound less than [Be3(OH)3(H2O2)5]2HR greater than 2+ was observed at pH 6.0-7.0. The cause of such intensive fluorescence was deformation of ion [Be3(OH)3(H2O)6]3+ because of its exclusion from the coordinating sphere of one molecule of water during the complex formation and decreasing of level H comes from II as compared to the morphocycline level II comes from n. A procedure for detecting morphocycline in the blood of humans and animals was developed.

Animals↗
PubMed1976

[Fluorimetric determination of rubomycin with the boric acid reaction].

A fluorimetric method for determination of rubomycin in the blood, milk and urine of humans and animals in concentrations of 0.04-20 gamma/ml was developed. After protein precipitation from the biological material with trichloracetic acid and the antibiotic was determined by fluorescence of its borate complex in concentrated sulfuric acid (gammaexcitk = 575, gammaflk = 620 nm).

Boric Acids↗
PubMed1976

[Fluorimetric determination of rubomycin].

A fluorimetric method for determination of rubomycin in the blood, milk and urine of humans and animals was developed. After precipitation of proteins from the biological material with 30 per cent trichloracetic acid the antibiotic was recovered from the centrifugate with isoamylic alcohol and the extract was subjected to fluorimetric analysis at gamma-exc-495 and gamma-fi-595 nm.

Chromatography, Paper↗