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Biomedical subjects

N M Tauraso

Publications and source records attributed to N M Tauraso.

At least 37 records · Page 2Linked to original sources
PubMed1971

Statistical evaluation of diluents and automatic diluting and pipetting machines in influenza serology.

The use of three diluents (i.e., 0.01 m phosphate-buffered saline, PBS; PBS with 0.2% gelatin, PBS/GEL; and PBS with 0.4% bovine plasma albumin) and three methods (i.e., the standard tube macro-procedure, TUBE; the manual microtechnique, MANUAL; and the semiautomatic microtechnique, AUTO) were statistically compared for their reproducibility and sensitivity in determining hemagglutinin (HA) and hemagglutination-inhibition (HI) antibody titers. In the HA test, analyses of between-cell variances of the different methods showed the AUTO microtiter procedure to be more reproducible than the standard TUBE method. The MANUAL microtiter procedure was the least reproducible. In the HI test, the TUBE method was the most reproducible. No significant difference in the reproducibility of the diluents was observed in either the HA or HI test. When a comparison of the sensitivity of test methods and diluents was made for determining HA titers, the AUTO microtiter procedure and PBS/GEL diluent appeared to be the method and diluent of choice. Evaluation of another instrument, the autopipetter, which standardizes the volume of diluent to be added in the microtechnique, suggests that the reproducibility of the AUTO microtiter procedure might be further increased.

Analysis of Variance↗
PubMed1971

Growth of respiratory syncytial virus in suspension cell culture.

Respiratory syncytial virus, Burnett strain, adsorbed efficiently and grew to high titers in suspension cultures of HEp-2 and MA-160 cells. Our results compared favorably with previous experience with the growth of respiratory syncytial virus in monolayer cell cultures. The use of suspension cell cultures provides a convenient and simple procedure for producing high-titering respiratory syncytial virus pools.

Adsorption↗
PubMed1971

Intracerebral inoculation of rhesus monkeys with a strain of measles virus isolated from a case of subacute sclerosing panencephalitis.

Measles virus isolated from the brain of a patient with subacute sclerosing panencephalitis was injected intracerebrally (ic) into 34 rhesus monkeys. Groups of these animals were injected with measles antigen in Freund's complete adjuvant or treated by schedules used for suppression of the general or cell-mediated immune responsiveness. In another group of animals, experimental allergic encephalitis was induced parallel with measles infection. Measles virus was isolated from the brains of monkeys up to 13 days after ic inoculation. No virus was detected in the central nervous system after 3 to 4 weeks, the longest postinoculation period examined. It was concluded that the subacute sclerosing panencephalitis-derived virus either lost its neurotropic properties at the passage level at which it was used or that it submerged into a silent stage and escaped detection. Neither immunosuppression nor concomitant autoimmune encephalitis had an effect on the survival of measles virus in the central nervous system. The histology of the nervous tissue was basically normal except for characteristic lesions of experimental allergic encephalitis in animals receiving the respective treatment.

Animals↗
PubMed1971

Serological responses and results of natural infectious challenge of recipients of zonal ultracentrifuged influenza A2-Aichi-2-68 vaccine.

In an epidemic of Hong Kong influenza in a penal institution, acute respiratory illness occurred in 5.6% of 196 inmates given single subcutaneous doses of 332, 535, or 1 265 CCA of zonal centrifuged, inactivated vaccine and in 11.7% of 755 nonvaccinated inmates (P<0.03). The frequency of illness among inmates who received single doses of 54 or 137 CCA of vaccine was not significantly different from that among the nonimmunized inmates (chi(2)=0.93, not significant). The vaccine was prepared with the A2/Aichi/2/68 strain of influenza and administered 7 weeks before the outbreak. Virus shedding was significantly lower in vaccinated inmates with respiratory disease (11.1%) than in nonvaccinated inmates (73.7%, P<0.04).Serological studies showed an increasing mean titre and increasing frequency of 4-fold or greater haemagglutination inhibition and neutralizing antibody responses with increasing dosage of vaccine.

Antibody Formation↗
PubMed1970

Effect of formalin, beta-propiolactone, merthiolate, and ultraviolet light upon influenza virus infectivity chicken cell agglutination, hemagglutination, and antigenicity.

Four strains of influenza virus were treated with Formalin, Merthiolate, Merthiolate and Formalin, ultraviolet light, and beta-propiolactone (BPL) for 18, 48, and 72 hr. Infectivity, chicken cell agglutination (CCA), hemagglutination (HA), and antigenicity determinations were made. Except for Merthiolate, each method of inactivation was equally effective in reducing infectivity. Loss of infectivity was related to length of treatment. CCA determinations were higher for all treated groups except for BPL-treated samples; these had lower determinations. BPL treatment also lowered the HA titer. Antigenicity was lessened by BPL treatment and by Merthiolate and Formalin treatment. Generally, the length of inactivation up to 72 hr did not affect CCA, HA, or antigenicity determinations. For the most part, there was no significant differences in the reactivity of the four strains.

Agglutination↗
PubMed1970

Evaluation of three cell culture systems as substrates for influenza virus assay.

The propagation of various influenza virus strains in primary rhesus monkey kidney (RMK), primary hamster kidney (HK), and the MDCK-USD canine kidney (CK) cell cultures was compared. Virus-infected cultures were examined for cytopathic effect (CPE) and by performing the hemadsorption (HAd) technique. The highest HAd titers were found most often in HK, followed by RMK and CK. However, the CK cell line provided the best substrate for detecting CPE. Although influenza B strains tended to grow to higher titers, these strains produced less CPE than did the A strains.

Animals↗
PubMed1970

Comparison of the haemagglutinin production of influenza A2 Hong Kong variant and recombinant strains.

Seven Hong Kong variant strains were compared for their capacity to produce haemagglutinin (HA). From the results of 9 serial egg passages made at 33 degrees C and 36 degrees C, the A2/Aichi/2/68 strain was found suitable as the vaccine production strain for the 1968-69 monovalent influenza vaccine manufactured in the United States of America.Results of initial experiments comparing the HA production by 2 Hong Kong variant recombinants and by the A2/Aichi/2/68 strain showed no essential difference among these strains. When these experiments were repeated starting with viruses that had undergone the same number of egg passages from isolation, the mean HA and chicken cell agglutination titres of the 3 recombinant strains used in these studies were only 1.5 times greater than those of the A2/Aichi/2/68 strain. By comparison, the HA producing capacity of A0/PR/8/34 was almost 6 times that of the A2/Aichi/2/68 strain.

Antigens↗