Biomedical subjects

N M Zholobak

Publications and source records attributed to N M Zholobak.

7 recordsLinked to original sources

PubMed2001

Virus-inhibitory effect of a yeast RNA-tilorone molecular complex in cell cultures.

The virus-inhibitory activity of a molecular complex (MC) of tilorone and yeast RNA was studied in vitro on three virus-cell systems: vesicular stomatitis virus (VSV) - murine fibroblast L929 cells, Venezuelan equine encephalittis virus (VEEV) - swine embryo kidney (SEK) cells and encephalomyocarditis virus (EMCV) - established piglet testicular (EPT) cells. In all these systems the MC exerted an antiviral effect similar to that of polynucleotide interferon (IFN) inducers such as poly(I)-poly(C), larifan and ridostin. The antiviral effect of the MC was similar when the compound was applied before or after virus adsorption to cells. The MC may be regarded as a perspective antiviral agent of common use.

Cells, Cultured↗

PubMed1999

[Comparative cytotoxic characteristics of the interferon-inducing yeast RNA-tilorone complex].

Cytotoxicity of the yeast RNA-tilorona molecular complex (MC) with interferonogenic properties and its influence on the DNA replicative synthesis were studied in experiments with human lymphocytes and 3 cell lines. It was shown that the MC doses of 25, 100 and 250 micrograms/ml were absolutely nontoxic for all the cell lines. The main parameters of the MC toxicity based on the cell viability were calculated. The parameters were found to correlate in the order of their magnitude with those relating to interferonogens of the polynucleotide nature. Within the dose ranges of 10 to 100 micrograms/ml the MC had a stimulating effect on replicative processes in the cells. It was concluded that the use of the MC as an inductor in large-scale manufacture of human and animal interferons of type 1 was promising.

Animals↗

PubMed1995

[The interferonogenic properties of yeast RNA-tilorone complexes in a cell culture].

The use of molecular complexes of yeast RNA with thyloron as inductors of type I interferons (alpha/beta-interferons) in the culture of cells L929 is described. It was shown that the complexes induced the interferon synthesis in the cells at the level comparable to that of the standard inductors of the polyribonucleotide nature i.e. larifan and poly(I)-poly(C). In the experimental doses the complexes proved to be nontoxic. It was concluded that the use of the yeast RNA and thyloron complexes as inductors in large-scale production of type I interferons was promising.

Animals↗

PubMedDate not supplied

[The microbial cenosis of the tonsils in healthy children and in those frequently ill].

The microbial cenosis of tonsils has been studied in healthy and ailing children. It has been found out that Staphylococcus aureus and S. epidermidis, beta-hemolytic streptococcus, rarely gram-negative flora and candidae were most often isolated in the ailing children. Normal microflora of healthy children mainly included lactic acid bacteria whose level considerably decreased in ailing children that is most distinctly expressed in children with decompensated tonsillitis. To correct the disturbances in microbial cenosis during complex therapy we used lactobacteria to treat the nasopharynx with the suspension of these bacteria. Positive clinical effect with following positive results of bacteriological investigation has been obtained in all patients.

Adolescent↗

PubMedDate not supplied

[Interferon inducers--from theory to practice].

The authors present up-to-date information concerning different inducers of different interferon types in vivo and in vitro. Interactions between the structure and interferon-inducing activity of high and low molecular weight inducers are in the focus of this paper. There are also a lot of data, concerning different biological properties of interferon-inducing compounds. Different aspects of inducers use as antiviral and immunomodulating drugs are also discussed.

Animals↗

PubMedDate not supplied

[Interferonogenic activity of immobilized ribopolynucleotides in vitro].

In vitro experiments the authors have studied a property of yeast RNA--tilorone hydrochloride complex covalently linked to spheron to induce the synthesis of interferons type I (alpha- and beta-interferons) in the culture of peripheral mononuclear human cells. Such a complex is shown to possess a marked interferonogenic activity. The data obtained appear to be a proof of the interferon induction to be realised by a mechanism needing at the first stage the contact between the inducer and the cell surface without its penetration into the cell.

Fungal Proteins↗

PubMedDate not supplied

[Production of type I interferons in the body exposed to yeast RNA-tiloron molecular complexes].

Molecular complexes forming as a result of interaction between yeast RNA preparations with 2,7-bis[-(diethylaminoethoxy)-fluorene]-9-on dihydrochloride (tilorone) administered parenterally to mice cause the appearance of interferon in high titers compatible to those induced by standard inductors of polyribonucleotide origin, poly(I)-poly(C) and larifan. Some physiologic conditions of interferonogenesis have been studied. The above molecular complexes in the dose range used experimentally were completely nontoxic. Hence, these complexes are promising agents for interferon induction.

Animals↗