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N Mills

Publications and source records attributed to N Mills.

22 records · Page 2Linked to original sources
PubMedDate not supplied

Effects of ethane dimethane sulfonate on the functional structure of the adult rat testis.

In ethane dimethane sulfonate (EDS)-treated adult Sprague Dawley rats, Leydig cells (LC) were not present up to 14 days but seen at 21 days. They increased in number thereafter and reached the values of age-matching controls (i.e., 150-day-old untreated) at day 60. Mesenchymal cell number per testis also increased and reached a peak at day 21, and remained at a higher (p<.05) value than the controls at days 28-60. LC were smaller at day 21, but were larger at days 28-60 (compared to untreated 90- and 150-day-old rats) and secreted more testosterone at day 60 compared to both control groups. Testes of treated rats had greater numbers of macrophages (except at day 28) and they were smaller than those in untreated rats and 60-day EDS rats. Immunolabeling studies on 3beta-HSD, 11beta-HSD1, and LH receptor activity and androgen data agreed with morphological findings. The relationship between mesenchymal and LC numbers during LC differentiation following EDS treatment is reminiscent of this process in prepubertal testis. The presence of increased numbers of macrophages in treated testes agreed with the role of macrophages on LC differentiation. The absence of aging signs in LC of 60-day treated rats who were 150 days of age can be attributed at least in part to their newly differentiated status in older rats (i.e., equivalent to pubertal LC and not to aged LC). Larger LC observed in EDS rats at days 28-60 and their increased testosterone secretory capacity at day 60 (compared to controls) are attributed to elevated plasma LH levels and locally produced factors in EDS rats.

11-beta-Hydroxysteroid Dehydrogenases↗
PubMedDate not supplied

The effect of positioning on arterial oxygenation in children with atelectasis after cardiac surgery.

OBJECTIVE: To determine the effect of body position on arterial oxygenation in children with unilateral atelectasis after cardiac surgery. DESIGN: Prospective, quasi-experimental, random assignment. SETTING: Midwestern university-affiliated tertiary pediatric medical center. PATIENTS: 25 children who underwent cardiac surgery and who presented with unilateral atelectasis within 2 weeks of operation. Age range was one month to 10 years (mean 34 months). OUTCOME MEASURES: The partial pressure of oxygen. INTERVENTION: Data collection was initiated within 24 hours of the diagnosed unilateral atelectasis. Arterial blood gases were drawn from intraarterial lines after subjects were placed for 15 minutes in the supine, right lateral, and left lateral decubitus positions (atelectatic lung dependent or nondependent), the order being randomized. RESULTS: Analysis of variance for repeated measures was used in the data analysis. The mean PaO2 for the supine, nondependent, and dependent positions were 115, 118, and 112, respectively. No statistical differences at p less than 0.05 level of significance were demonstrated for the body positions under study. Age and degree of atelectasis were analyzed as covariates to determine the possible correlation with the PaO2 and the change in PaO2. Age inversely correlated with the PaO2, r = -0.24 (p less than 0.05), indicating the older subjects had a lower PaO2. The degree of atelectasis demonstrated correlation with the change in PaO2, r = -0.26 (p less than 0.05) indicating the subjects with greater degree of atelectasis had a lesser change in PaO2. CONCLUSIONS: These results differ from similar studies on the effect of positioning in adult subjects. This finding suggests that the effect of positioning of children who have had cardiac surgery should be evaluated on an individual basis with close monitoring for changes in clinical condition and oxygen saturation and periodic arterial oxygen blood sampling until further studies can provide conclusive direction.

Age Factors↗
PubMedDate not supplied

Management of thrombotic complications of invasive arterial monitoring of the upper extremity.

Invasive arterial monitoring is essential intraoperative and perioperative management of vascular and cardiac patients. Complications resulting from arterial monitoring, although rare, can result in severe functional sequelae and loss of an extremity. Severe extremity ischemia and repeated thromboses in a 10-year-old male resulted from brachial arterial monitoring line. Multiple operative thrombectomies and systemic anticoagulation were unsuccessful. Limb salvage and arterial patency were achieved by saphenous vein patch angioplasty at site of arterial injury and intermittent intra-arterial streptokinase infusion placed into a natural side branch of the saphenous vein segment. Although many complications may result from arterial monitoring, the most serious is arterial thrombosis with impending digital or extremity gangrene. This constitutes a surgical emergency. When aggressive conventional surgical techniques alone fail to control catheter induced thrombosis, an innovative technique in combination with regional intra-arterial streptokinase therapy has been successful adjunctive measure in reestablishing microvascular patency resulting in limb salvage.

Arm↗