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Biomedical subjects

N Morimoto

Publications and source records attributed to N Morimoto.

At least 37 records · Page 2Linked to original sources
PubMed2000

Immunolocalization of transforming growth factor-betas and their receptors in the intervertebral disk of senescence-accelerated mouse.

The temporal and spatial immunolocalization of TGF-betas and their receptors in the intervertebral disk of senescence-accelerated mouse (SAM) was examined to determine the biological roles played by TGF-betas and their receptors in the process of intervertebral disk degeneration. Ten male SAM and ICR mice aged 8, 24 or 50 weeks after birth were used for this experiment. Histological and immunohistochemical studies using specific antibodies for TGF-beta1, -beta2, -beta3, TbetaR-I, and TbetaR-II were performed. Intervertebral disks of SAM exhibited more degenerative changes than those of ICR mice. Expression of TGF-betas and TbetaRs in disk of SAM and ICR mice was observed at 8 weeks of age, and became weaker with aging. Our results suggest TGF-betas may play a role in the growth and maintenance of intervertebal disks.

Aging, Premature↗
PubMed2000

[Sudden cardiac arrest after induction of general anesthesia: a case report].

We experienced sudden cardiac arrest after induction of general anesthesia using isoflurane. The patient had had paroxysmal atrial fibrillation for one year and had been treated with digoxin and cibenzoline succinate. Sinus rhythm appeared soon after the start of closed chest compression. However cardiac arrest recurred, and we inserted a temporary pacemaker catheter to stabilize the circulatory status. She awoke from anesthesia without any complications. The diagnosis of sick sinus syndrome (SSS) was made postoperatively and she had a permanent pacemaker implanted. We thought that the hidden SSS had been the cause of this sudden cardiac arrest.

Aged↗
PubMed2000

[Postoperative pancreatitis after total hip replacement under general anesthesia].

A 61 year old male patient developed postoperative pancreatitis after total hip replacement under general anesthesia with sevoflurane. The patient had chronic renal failure and was receiving hemodialysis. The estimated intraoperative blood loss was 1500 ml, and 1200 ml of blood was administered. The intraoperative mean blood pressure was 60 to 70 mmHg and the central venous pressure at the end of anesthesia was 0 mmHg. Postoperatively he complained of severe upper abdominal pain. On the 1st postoperative day serum amylase level increased to fifteen times of the normal level. He complained again of severe abdominal pain on hemodialysis. From these episodes, we estimate that the circulatory disturbance of pancreas is the cause of this postoperative pancreatitis.

Acute Disease↗
PubMed1999

Immunolocalization of bone morphogenetic protein and its receptors in degeneration of intervertebral disc.

STUDY DESIGN: Immunohistochemical study of expression and localization of bone morphogenetic protein (BMP)-2/4 and type I and II receptors on intervertebral disc. OBJECTIVES: To determine the biologic functions of BMPs and their receptors in the process of degeneration of the intervertebral disc. SUMMARY OF BACKGROUND DATA: Biologic and pathologic processes in the cell during the degeneration of the intervertebral disc are as yet poorly understood. METHODS: The cervical spines of 15 male senescence-accelerated mice aged 8, 24, or 50 weeks were used for histologic and immunohistochemical examination of BMP-2/4 and BMP receptors IA, IB, and II. Immunostaining was performed with the avidin-biotin-peroxidase complex method. RESULTS: Degenerative change was recognized within intervertebral discs of senescence-accelerated mice aged 50 weeks. BMP-2/4 and its receptors were abundant in hyaline cartilaginous cells within the endplate of the vertebrae at 8 and 24 weeks of age. However, the expression of BMP-2/4 and its receptors moved from the hyaline cartilage of the endplate of the vertebrae to fibrous cells within the anulus and to the calcified cartilage at the site of enthesis of mice aged 50 weeks. CONCLUSIONS: BMP-2/4 and its receptors may play roles in degenerative change of intervertebral disc.

Aging↗
PubMed1999

Early detection of radiation-induced liver injury in rat by superparamagnetic iron oxide-enhanced MR imaging.

The detectability of early liver injury induced by irradiation was studied using magnetic resonance (MR) imaging enhanced with superparamagnetic iron oxide (SPIO), a tissue-specific contrast agent against the reticuloendothelial system (RES). In rat, 3 days after focal irradiation (0-10 Gy), MR imaging was performed and specimens were obtained to observe the phagocytic function of RES. The irradiated portion of the liver was visualized with a clear demarcation from the nonirradiated part by SPIO-enhanced MR images as a decrease in negative enhancement reflecting the function of RES (P < 0.05), whereas this was impossible with nonenhanced MR images. Significant regression was observed as a dose-related change of the signal intensity in the irradiated portion on SPIO-enhanced MR images (R = 0.867, P < 0.0001). SPIO-enhanced MR imaging was reliable for detecting the range and extent of liver injury a few days after low-dose irradiation, and it may be a useful procedure for verifying the target area in clinical cases of radiation therapy.

Animals↗
PubMed1999

Three-dimensional computed tomographic angiography in lower extremity revascularization.

To evaluate the role of three-dimensional computed tomographic angiography (3D-CT) in peripheral artery bypass surgery, 49 patients with chronic lower limb ischemia underwent 3D-CT before and after a bypass operation. 3D-CT had a sensitivity of 96.8%, a specificity of 95.0% and an overall accuracy of 96.1% for the diagnosis of arterial and graft stenosis or obstructions. The ability to observe the acquired images at any angle was very useful for assessing the implanted graft in both aortoiliac and infrainguinal bypasses. Although these images equally identified arterial stenosis, obstruction, and the anastomotic morphology of bypass grafts as well as conventional angiography, the diagnostic accuracy was not helpful in the small arteries and grafts. 3D-CT is a low-invasive imaging method that is sufficient for forming strategies for bypass operations. In aortofemoral or femoropopliteal bypass surgery, 3D-CT thus provides sufficient imaging accuracy for bypasses up to the popliteal artery below the knee in patients with chronic limb ischemia.

Aged↗
PubMed1999

Small hepatocellular carcinoma: relationship of signal intensity to histopathologic findings and metal content of the tumor and surrounding hepatic parenchyma.

PURPOSE: To investigate the relationship between the metal content of hepatocellular carcinoma (HCC) and the signal intensity pattern on magnetic resonance images. MATERIALS AND METHODS: The signal intensity patterns of 59 HCCs 3 cm in diameter or smaller were correlated with histologic findings and metal content. RESULTS: HCCs with high signal intensity on T1-weighted images demonstrated more steatosis (P = .035) and higher copper content (P = .008) than did surrounding hepatic parenchyma. HCCs with high signal intensity on T2-weighted images demonstrated more clear cells (P = .001) than did surrounding hepatic parenchyma. The higher signal intensities on T1-weighted and T2-weighted images were related to a higher and a lower degree of histologic differentiation, respectively. Multivariate analysis showed that the contrast-to-noise ratio between the HCC and surrounding hepatic parenchyma was affected by intratumoral copper content (P = .0338), zinc content of surrounding hepatic parenchyma (P = .0379), and the degree of histologic differentiation on T1-weighted (P = .0031) and T2-weighted (P = .0062) images. CONCLUSION: The signal intensity of HCC on T1-weighted images is related to the degree of histologic differentiation, intratumoral copper content, and zinc content of surrounding hepatic parenchyma, whereas the signal intensity on T2-weighted images is related to the degree of histologic differentiation.

Adult↗
PubMed1999

The features of psychological problems and their significance in patients on hemodialysis--with reference to social and somatic factors.

AIM: For the purpose of identifying the features of psychological problems and their significance in patients on hemodialysis, we analyzed how psychological problems are affected by social and somatic factors. SUBJECTS AND METHODS: The subjects all consisted of patients on hemodialysis at the Kidney Center of Fukuoka Red Cross Hospital between December 1994 and December 1996. We used the Cornell medical index health questionnaire on neurosis and the "easily upset or irritated", State-trait anxiety inventory to determine both state and trait anxiety, the Self rating Depression Scale on depression and divided into the patients who demonstrated each psychological problem and those who did not, and then analyzed the psychological problems between the two groups with reference to somatic and social factors which may have led the patients to develop their respective psychological problems. RESULTS: According to a chi-square analysis, neurosis, clinical trait anxiety and clinical state anxiety were all more closely related to somatic factors than to social factors. In contrast to neurosis and anxiety, more social factors than somatic factors were related with "easily upset or factors were related with" easily upset or irritated". "Easily upset or irritated" was significantly related to living with a spouse or with children. In addition, depression was related to various factors including both somatic and social factors. In a stepwise multiple logistic regression analysis, the presence of restless legs also correlated with all the psychological symptoms investigated in this study. The prevalence of depression was also related to the degree of awareness regarding the cause of renal failure (p < 0.01). CONCLUSION: Our results thus revealed the features of the psychological problems and their significance in patients on hemodialysis.

Anxiety↗
PubMed1999

[Evaluation of the ketamine maintenance dose in anesthesia using propofol, ketamine and nitrous oxide].

We studied the influence of the ketamine maintenance dose on propofol infusion speed, blood pressure change and recovery time in anesthesia using propofol, ketamine and nitrous oxide. Anesthesia was maintained with ketamine 0.6 or 0.2 mg.kg-1.hr-1. The ketamine maintenance dose exerted no influence on propofol infusion speed, the occurrence of hypertension or hypotension. The recovery time correlated with the total amount of ketamine. From these results we conclude that 0.2 mg.kg-1.hr-1 is an appropriate maintenance dose of ketamine in anesthesia using propofol, ketamine and nitrous oxide.

Aged↗
PubMed1998

Treatment of primary varicose veins: an assessment of the combination of high saphenous ligation and sclerotherapy.

This study was conducted to determine the optimal treatment for minimizing the recurrence of varicose veins, and to assess the value of the combined treatment of high saphenous ligation and sclerotherapy. A combined total of 967 limbs in 660 patients with primary varicose veins were treated. The treatments comprised: sclerotherapy alone in 81 limbs, high saphenous ligation alone in 3 limbs, a combination of sclerotherapy and high saphenous ligation in 843 limbs, and a combination of sclerotherapy and ligation of incompetent perforating veins in 10 limbs. Recurrent varicose veins appeared from 3 months after the initial treatment in 61 (6.3%) limbs. Saphenous-type recurrence, being the presence of saphenofemoral incompetence (SFI) and/or saphenopopliteal incompetence (SPI), was found in 20 limbs, and further treatment was given. Segmental-type recurrence, being superficial varices without SFI or SPI, was found in 41 limbs, all of which were treated with further sclerotherapy alone. To minimize the recurrence rate of varicose veins, the presence or absence of SFI, SPI, and incompetent perforating veins must be confirmed prior to the initial treatment. It was concluded that the combination of high saphenous ligation and sclerotherapy is effective for patients with SFI and/or SPI, whereas sclerotherapy alone is better for patients without SFI or SPI.

Adult↗
PubMed1998

Anaesthetic management of caesarean section in a patient with myelodysplastic syndrome.

PURPOSE: This case report describes the anaesthetic management for Caesarean section in a patient with myelodysplastic syndrome. CLINICAL FEATURES: A woman with myelodysplastic syndrome underwent Caesarean section on two occasions. The first Caesarean section was performed at age 20 yr using general anaesthesia with nitrous oxide-oxygen and fentanyl. In her second pregnancy at 25 yr, there was severe pancytopenia at 28-wk gestation with a leukocyte count 3.6 x 10(9).L-1, erythrocyte count 1.2 x 10(12).L-1, haemoglobin 50 g.L-1, haematocrit 14.7% and platelet count 51 x 10(9).L-1. Following leukocyte poor red cells and platelet transfusion, general anaesthesia was maintained with nitrous oxide-oxygen-sevoflurane and fentanyl. Both operations were uneventful and healthy infants were delivered. CONCLUSION: It is important to have a team approach (anaesthetist, obstetrician and haematologist) for the perianaesthetic management of patients with myelodysplastic syndrome. An exact assessment of the haematological condition, the need for prophylactic treatment and anaesthetic management should be determined for each individual patient.

Adult↗
PubMed1998

Relationship between masticatory muscle activity and vertical craniofacial morphology.

The purpose of this study was to investigate the relationship between masticatory muscle activity during the day and vertical craniofacial morphology. The sample comprised 30 subjects (20 males and 10 females, age range 15 to 28 years, mean 24 +/- 3.2 years) who had normal anteroposterior skeletal relationships and complete or nearly complete dentition without serious malocclusion or temporomandibular dysfunction. Using a portable electromyographic recording system, activities of the masseter, temporal, and digastric muscles were recorded for 3 hours during the day, excluding time spent eating, sleeping and exercising. A lateral cephalogram was taken of each subject with the teeth in occlusion. Activities of the masseter, temporal, and digastric muscles consisted mainly of low-amplitude bursts. The duration of digastric muscle activity was greater than that of either the masseter or temporal muscles. Masseter and digastric muscle activity was positively correlated. The activities of the masseter, temporal, and digastric muscles during the day consist of low-amplitude bursts and may be related to vertical craniofacial morphology.

Adolescent↗
PubMed1997

[Poliovirus (Sabin strain) multiplication in human intestinal tract after oral polio vaccination].

Fecal specimens from a baby vaccine were collected every day from 1 to 51 days after primary vaccination and from 0 to 15 days after secondary vaccination. Polioviruses were isolated with GMK-2 cell line from 10% emulsion of the feces and titrated the virus contents in the emulsion of the feces. The isolated viruses were tested the reproductive capacity at 39.0 degrees C and 39.5 degrees C by the plaque method with primary cynomologous monkey kidney cells. Viruses were isolated from the feces during 28 days for type 1, 39 days for type 2 and 36 days for type 3 after primary vaccination, however, only type 1 viruses were isolated during 7 days after secondary vaccination. The multiplication of type 3 viruses in the intestine were increased after diminished the multiplication of type 1 and type 2. In plaque formation capacity at 39.0 degrees C and 39.5 degrees C, the isolates had shown to differ clearly among the types of poliovirus. After primary vaccination, type 1 isolates were not produced the plaques at 39.0 degrees C and 39.5 degrees C. Although type 2 isolates were not formed the plaques until the 14th day at 39.5 degrees C, the plaque formation capacity of the these isolates were increased gradually i.e.; on the 20th day (10(0.88) PFU/ml), the 26th day (10(2.00) PFU/ml) and the 39th day (10(2.63) PFU/ml) at 39.5 degrees C, and all of type 2 isolates tested were showed the plaque formation capacity (10(2.88 approximately 10(3.76) PFU/ml) at 39.0 degrees C. Type 3 isolates were formed plaques at 39.0 degrees C and 39.5 degrees C from the 7th day. After the secondary vaccination, type 1 isolates (7th day) was a little changed them. Neutralizing antibody titers were shown that type 1 was 320, type 2 was 110 and type 3 was 60 after 1 year of the second administration. These titers were closely similar the geometric mean titers of 2 year old babies in Japan.

Humans↗
PubMed1997

[The effect of vasodilator on the occurrence of postoperative shivering and the fall of core temperature].

We evaluated the effect of intraoperative vasodilator therapy on the occurrence of postoperative shivering and the fall of core temperature during 37 abdominal operations by the stepwise multiple regression analysis. As the vasodilator, we used PGE1 at the dose of 0.02-0.05 microgram.kg-1.min-1. In the vasodilator group, postoperative peripheral surface temperature was high and the occurrence of shivering was low. And intraoperative and postoperative core temperatures were not affected by that therapy. From these results, we conclude that intraoperative vasodilator therapy suppressed the occurrence of postoperative shivering without a fall of core temperature.

Adult↗
PubMed1997

[A malpositioned CVP catheter].

A 2-year-old boy was scheduled for patch closures of ASD and VSD. After anesthesia induction, infection of a double lumen central venous catheter (5 Fr, Arrow) was tried into the superior vena cava through the right jugular vein by Seldinger's method. We confirmed the placement of the catheter by drawing a small amount of blood. After the operation, chest X-ray examination in ICU revealed the misplacement of the catheter into his right intrapleural space. The catheter was left overnight to be used as a drainage route of a possible bleeding. Next morning, no abnormal finding in his chest X-ray and stable circulatory and respiratory conditions were found and we proceeded to extubate his endotracheal tube and take away the catheter. Two hours after the removal of the catheter, the boy showed forced respiration. He became cyanotic rapidly and then he needed emergency intubation. Following chest X-ray examination and an aspiration of intrapleural space revealed a severe hemothorax of the right side, where catheter had been inserted. The boy recovered without any disorders. This case suggests the importance to confirm the placement of CVP catheter, and to prevent the possible complications due to the malpositioned catheter.

Anesthesia, General↗
PubMed1996

Masseter muscle activity during the whole day in young adults.

This study was conducted to evaluate the biting function during the entire day in young adults by measuring the masseter muscle activity with a 24-hour electromyography (EMG) system. With this system, the masseter muscle activity for 24 hours was recorded in 20 male and 10 female young adult subjects with acceptable occlusion. For the male subjects, means of the number and total duration of bursts over level 2, which is one-fourth of the maximum discharge voltage during maximum clenching, were 1938 times and 357 seconds. For the female subjects, the values were 2356 times and 419 seconds. A large percentage of such high amplitude bursts as level 3 or 4 was observed during meals in both groups. These results indicated that most of the strong bursts of the masseter muscle appeared only during meals and a number of low amplitude bursts were observed during the entire day, although masseter muscle activity during the entire day in young adults was less than expected. It is anticipated that the nature of masticatory muscle activity revealed in this study might provide valuable information about the association between the biting function and the craniofacial structure or malocclusion.

Activity Cycles↗
PubMed1996

A study of force application, amount of retarding force, and bracket width in sliding mechanics.

We investigated the relationship of the retraction force to the location of force application, retarding force and bracket width during simulated sliding tooth movement along an arch wire. Point 1 for retraction was located at the center of the bracket, and points 2 and 3 were at 4.0 mm and 6.0 mm from the bracket slot, respectively. Weights of 100 gm, 200 gm, and 400 gm were suspended at 9.0 mm from the bracket slot as the point of simulated center of resistance. Stainless steel standard edgewise wide, medium, and narrow twin brackets were engaged with two elastomeric ligatures on a stainless steel wire (0.016 x 0.016 inch). The bracket was retracted at the rate of 0.1 mm per second for a distance of 2.0 mm. Measurements were repeated six times, and the results were compared with multiple ANOVA tests. For all brackets, with an increase of the retarding weight, the mean retraction force at points 1 and 2 increased but decreased at point 3. The mean retraction force at point 1 for the narrow twin bracket was significantly (p < 0.05) higher than that for the wide twin bracket at all retarding force levels. However, the mean retraction force at points 2 and 3 for the narrow twin bracket was significantly (p < 0.05) lower than for the wide twin bracket at all retarding force levels. These findings indicated that the point of force application, the resistance force of a tooth, and the width of the bracket are crucial in consideration of the tipping moments on the bracket.

Analysis of Variance↗