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N N Besednova

Publications and source records attributed to N N Besednova.

10 recordsLinked to original sources
PubMed1992

[Combined action of doxycycline and mytilan on the immune response to tularemia antigen].

Combined action of doxycycline and mytilan, a natural polysaccharide, on the primary immune response to the antigen of the tularemia vaccinal strain in CBA mice was studied. The polysaccharide was used to compensate the immunosuppressive effect of doxycycline high doses on the humoral immune response. The maximum stimulation of the antibody titers as compared to the controls (more than 250 per cent) was observed when mytilan was administered simultaneously with or prophylactically 3 days prior to the antibiotic in doses of 2.5 and 25 mg/kg. The use of mytilan in combination with doxycycline high doses made it possible to compensate the antibiotic-induced decrease of DTH and even to stimulate it as compared to the controls. The highest levels of DTH (150 per cent against the control) were observed when mytilan was administered prophylactically in doses of 2.5 and 11.25 mg/kg 3 days prior to immunization. Mytilan had the highest stimulating effect on antibody production. The combined use of doxycycline and mytilan was characterized by significant stimulation of antibody production and DTH when the dose/time regimens were rational.

Animals↗
PubMed1979

[Immunostimulating activity of the lipopolysaccharides of blue-green algae].

The whole cells of blue-gree algae and lipopolysaccharides isolated from these cells were shown to stimulate the production of macro-(mainly) and microglobulin antibodies in rabbits. The macro- and microphage indices in rabbits increased significantly after the injection of LPS isolated from blue-green algae 24--48 hours before infecting the animals with a virulent Y. pseudotuberculosis strain. Besides, the inhibiting action of this strain on the migration of phagocytes to the site of infection was abolished immediately after the injection. The use of the indirect hemagglutination test allowed to prove the absence of close antigenic interrelations between blue-green algae and the following organisms: Spirulina platensis, Microcystis aeruginosa, Phormidium africanum and P. uncinatum.

Adjuvants, Immunologic↗
PubMed1975

[A comparative immunochemical study of the subtypes of serologic type I of the agent of pseudotuberculosis].

It was shown that Y. pseudotuberculosis strains causing the Far-Eastern scarlatina-like fever in the Primorsk region belonged to subtype IB-ipopolysaccharides of the standard strain of subtype IB and of the local strain were closely affiliated by the analytic data and monosaccharide composition, but differed from the lipopolysaccharide of strains belonging to subtype IA. Living vaccines should be used to obtain the sera against the subtypes IA and IB of the causative agent.

Amino Sugars↗
PubMed1975

[Interaction of the causative agent of pseudotuberculosis with the peritoneal macrophages from an immune and a nonimmune organism].

The role of phagocytosis in experimental pseudotuberculosis was demonstrated in vivo and in vitro. Virulent Y. pseudotuberculosis strain caused the death of the majority of the cells, whereas the weakly-virulent ones--degenerative changes and the death of but of few of them. The capacity of the causative agents of pseudotuberculosis to survive and to reproduce within the cells could be regarded as one of the significant factors of virulence of these microbes. The maximal immunizing effect was observed in infection of the animals with sublethal dose of the living Y. pseudotuberculosis culture. A somewhat lesser, but a sufficiently high, immunizing effect was produced by lipopolysaccharide. A method of macrophage culture can be used for the assessment of the immunogenicity of various antigenic complexes of the microbes.

Animals↗
PubMed1975

[Several biological properties of the surface antigens of the agent of pseudotuberculosis].

Capsular substance and extracellular proteins of the causative agent of pseudotuberculosis caused in the organism of guinea pigs a specific immunological reconstruction with the accumulation in the blood of antibodies in low titres, chiefly of macroglobulin type. The blood sera of these animals failed to protect the mice from lethal pseudotuberculosis infection in experiments of passive protection. Surface antigens of pseudotuberculosis causative agents produced a sensitizing action on the guinea pig organism; this was shown in vitro with the macrophages of the abdominal cavity of these animals. Extracellular proteins decreased the completeness of phagocytosis by neutrophilic leukocytes (demonstrated in vitro with the donor blood leukocytes).

Animals↗