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Biomedical subjects

N N Bogomolova

Publications and source records attributed to N N Bogomolova.

At least 19 recordsLinked to original sources
PubMed1991

[Persistence of the measles virus genome in peripheral blood lymphocytes in patients with glomerulonephritis and systemic lupus erythematosus].

Punctate hybridization was used to examine measles virus genome in peripheral blood lymphocytes from patients with glomerulonephritis (GN) and systemic lupus erythematosus (SLE). Measles virus genome was revealed in 47 (54%) out of 87 GN patients, in 27 (74%) out of 36 SLE patients and in none of the control group patients. GN patients manifested a tendency towards increase of the rate of measles virus demonstration with the rise of the titres of measles antibodies. All SLE patients with a high antibody titre (1:512 and higher) demonstrated measles virus genome. The rate of measles virus genome demonstration in GN and SLE patients did not depend on the sex, age, duration and clinical disease pattern or the content of IgA, IgM, IgG in blood serum. During disease exacerbation, the rate of measles virus demonstration was higher in 41 (59.4%) out of 69 patients than during remission--in 6 (33.3%) out of 18 patients (p less than 0.05).

Adolescent↗
PubMed1990

[Detection of the measles virus genome in the peripheral blood lymphocytes of patients with chronic and acute glomerulonephritis].

The presence of the measles (rubeola) virus genome was searched for in the lymphocytes from the peripheral blood of patients suffering from the acute (16 persons) or chronic (164 persons) glomerulonephritis. Dot hybridization technique with the plasmid borne probes to the measles viral genes NP, P and H have been used for the search. The measles viral genome has been detected in 58% of lymphocytes from the patients with the chronic glomerulonephritis and in 50% of lymphocytes from the patients suffering from the acute form of the disease. The genome was not found in the material from the control group including donors and traumatology ward patients. 25 samples of lymphocytes from the patients with the chronic glomerulonephritis contained the RNA that was not hybridizable with the viral genes probes by dot hybridization technique, thus containing no genes homologous to parotitis viral genes. The average titer of anti-measles antibodies in the serum from patients with chronic glomerulonephritis the lymphocytes of which contained the measles viral genome was 1:304, while it was 1:154 for patients with the negative probes. The average anti-measles antibodies titers are the same (1:166 and 1:142) for analogical groups of patients with acute form of disease.

Acute Disease↗
PubMed1989

Detection of measles virus genome in blood leucocytes of patients with certain autoimmune diseases.

RNA isolated from lymphocytes of peripheral blood was dot-hybridized to a hybrid plasmid containing specific sequences for measles virus nucleocapsid protein. Viral RNA was detected in the lymphocytes of 28 of 34 (82%) patients with systemic lupus erythematosus (SLE) and of 40 of 68 (59%) patients with chronic glomerulonephritis (CGN), and was not detected in 29 control patients.

Antibodies, Monoclonal↗
PubMed1989

[Mechanisms of disrupting DNA repair in human cells. IV. Interferon protects DNA of noninfected and chronically infected human cells from damage caused by cadmium chloride].

The protective activity of interferon on the cadmium chloride-treated human cells (Hep-2), infected chronically with meals virus and uninfected, was studied. It was found that cadmium chloride induced the formation of partially non-repairable DNA lesions. Decrease in cell repair activity was observed in the cells chronically infected with virus. Pretreatment of cells with interferon protected cell DNA from formation of DNA breaks and caused more effective resynthesis of DNA breaks.

Cadmium↗
PubMed1987

Non-infectious morphologically altered nucleocapsids of measles virus from persistently infected cells.

Persistent measles virus infection of human HEp-2 or L-41 cells was accompanied by pronounced structural and functional changes of isolated intracellular viral nucleocapsids (NCs). The bulk of persistent NCs possessed altered conformation and a "string-of-beads" appearance, contained substantial amounts of subgenomic size RNAs, exhibited reduced transcriptase activity in vitro and lacked infectivity on transfection of susceptible cells. Immunogold staining revealed negligible binding of anti-P protein monoclonal antibodies to the "string-of-beads" type NCs, thus suggesting their non-functional state.

Capsid↗
PubMed1987

Pathomorphologic characterization of CNS damage in monkeys infected with persistent variant of measles virus vaccine strain L-16.

The lesions of CNS were examined in monkeys infected intracerebrally (i.c.) with a variant of measles virus vaccine strain L-16 isolated after prolonged persistence in human cell culture NEr-2. The persisting virus variant appeared pathogenic for monkeys. The changes which had developed in their CNS within 30 to 60 days post-infection (p.i.) were alike to acute measles encephalitis which was evidenced by giant cell formation at the injection site. Twenty-two months p.i. the chronic character of lesions was evident from the appearance of foci of neuron destruction. Based on morphologic findings it was suggested that strain L-16-H has acquired some properties characteristic of nonattenuated virus.

Animals↗
PubMed1986

Measles virus persistent infection: modification of the virus nucleocapsid protein.

The synthesis of intracellular measles virus proteins in persistently infected human cell cultures was studied. The virus-induced proteins were analysed after radioimmunoprecipitation by one- and two-dimensional polyacrylamide gel electrophoresis. The measles virus-induced nucleoprotein (NP) synthesized in persistently infected cells had a reduced binding capacity with measles virus antibodies (human convalescent serum) compared to the NP protein induced by the virus used to initiate the infection. In contrast, monospecific rabbit serum prepared against the original virus NP, or monoclonal anti-NP antibodies, precipitated NP proteins from acutely and persistently infected cells with equal efficiency. When the NP in acutely or persistently infected cells was labelled with either 14C- or 3H-amino acids and subjected to two-dimensional gel analysis, significant charge differences were observed between the virus proteins. When measles virus-infected cells were examined for virus protein synthesis at 40 degrees C, although no change was found in acutely infected cells, NP was not detected in the persistent infection.

Antibodies, Viral↗
PubMed1986

Stability of rubella virus after long-term persistence in human cell line.

Primary infection of HEp-2 cells with rubella virus resulted in non-cytophatic long-term persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. Both original and persistent rubella viruses were thermoresistant (T 56 degrees C) and slightly temperature-sensitive. Clonal analysis revealed presence of ts-mutants among both original and persistent virus clones with different degrees of plating efficiency at 40 degrees/34 degrees C. RNA fingerprinting showed only minor changes in persistent rubella virus.

Antigens, Viral↗
PubMed1983

Myxoviruses do not induce non-specific alterations in membrane permeability early on in infection.

The permeability characteristics of cells infected with myxoviruses have been studied by measuring the concentrative uptake of nutrients, the concentration of intracellular K+, and the maintenance of the Na+ gradient across the plasma membrane. Cells either show no change at all (Sendai virus-infected BHK cells and measles virus-infected Vero cells) or they show a decreased ability to concentrate nutrients, while intracellular K+ and the Na+ gradient remain unchanged (Sendai and influenza virus-infected L-1210 cells, measles virus-infected lymphocytes and mumps virus-infected L-41 cells). In no case, therefore, was a change observed that resembles the non-specific increase in membrane permeability induced by haemolytic paramyxoviruses (35, 42) or the non-specific membrane leakiness postulated to take place in infected cells (8, 9). A preliminary account of some of these findings has been presented (39).

Animals↗
PubMed1983

Chronic non-cytopathic infection of human continuous cell lines with mumps virus.

Chronic infection with a vaccine strain of mumps virus (MV) was produced and studied in human cell cultures L-41 and HEp-2. The establishment and course of the infection was not accompanied by cytopathic changes. Among probable protective factors (mechanisms) interferon (IFN) was detected in L-41 culture and defective interfering particles in either cell culture. Their role in the establishment and maintenance of chronic infection was not confirmed, however.

Animals↗
PubMed1981

Comparative study of rabies virus persistence in human and hamster cell lines.

Persistent infections by rabies virus in BHK-21/13S and HEp-2 cells were studied comparatively. No evidence of interferon production, selection of virus-resistant cells, or integration of the viral genome could be found. Persisting viruses replicated efficiently at 34, 36, and 40 degrees C. Both persistently infected cultures released defective interfering virus particles. A cyclical pattern of infection, which was not characteristic of the persistently infected HEp-2 system, was observed in persistently infected BHK cultures. The virus from persistently infected BHK cultures lost its virulence for mice, whereas the virus from persistently infected HEp-2 cultures retained mouse-killing capacity for more than 3 years.

Animals↗
PubMed1979

A comparative study of tick-borne encephalitis virus RNA synthesis in acutely and persistently infected cells.

Rate zonal and buoyant density gradient centrifugation did not reveal any difference between tick-borne encephalitis virus virions released from acutely or persistently infected cells. All three RNA species characteristic for flavivirus replication were found both in acutely or persistently infected cells, but increased levels of intracellular 42S RNA polyadenylation was observed in persistently infected cells.

Animals↗