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Biomedical subjects

N N Klemparskaia

Publications and source records attributed to N N Klemparskaia.

At least 19 recordsLinked to original sources
PubMed1991

[Purified sulfur as an agent to relieve the side effects in the radiation therapy of cervical cancer].

The theoretical basis of this work is the development of autosensitization in exposure to ionizing radiation and well-known desensitizing action of sulphuric agents. To reduce clinical manifestations of a reaction to combined radiotherapy 34 women with diagnosis of cervical cancer (stages I and II) were given 0.5-1 g of purified sulphur mixed with 0.25 g of glucose in the morning every 2-3 hours before irradiation, per os; 24 patients received placebo (a mixture of egg powder with glucose), in 21 patients no protective means were used. All 79 patients were given unified adjuvant therapy (polyvitamins, leukogens, folic acid) and diet No 15. A significant decrease in the reaction to therapeutic irradiation was noted in the study group. No side-effects were observed.

Animals↗
PubMed1986

[New data on the biological action of normal immunoglobulins].

Experiments on 2,520 CBA mice (CBA X X C57BL) F1 nice have shown that the injection of homologous serum immunoglobulins (obtained from intact and blood-stimulated animals), made 2 hours after gamma irradiation from a 60Co source, prevents the development of intestinal dysbacteriosis and endogenous infection. The injection of mouse and human immunoglobulins to nonirradiated mice improved their resistance to experimental infection with Escherichia coli live culture, increased the expression of receptors to the Fc-fragments of IgG in peritoneal macrophages and enhanced the physical working capacity of the animals. The preparations containing normal antitissular antibodies have proved to be particularly effective. In mice, rabbits and dogs the preparations under test have produced no changes in the general state of the animals, no local reactions and no disturbances in the cardiovascular activity.

Animals↗
PubMed1984

[Effect of homologous serum globulin preparations on the intestinal automicroflora of mice after irradiation].

In experiments on 4000 noninbred and CBA mice the influence of the injections of alpha-, beta- and gamma-globusin, IgG and IgM, obtained from the sera of hemostimulated and intact mice, on the intestinal microflora after irradiation has been studied. The experiments have revealed that 3 subcutaneous or intraperitoneal injections of 1 mg per mouse, made 2, 24 and 48 hours after the irradiation of the animals with gamma-rays in a dose of 700 r, considerably reduce the intensity of the accumulation of opportunistic bacteria in the small and large intestines, commonly occurring in irradiated animals. A decrease in the number of lactobacteria is less pronounced. The preparations of globulin and IgG obtained from hemostimulated mice, i.e. enriched with normal tissue antibodies, have proved to be most effective.

Animals↗
PubMed1978

[Further study of the nature and characteristics of the rosette formation phenomenon by a modified Jerne's method].

Experiments were conducted on 105 mongrel female albino mice weighing 18--22 g (intact, irradiated in a dose of 700 r with gamma-rays 60Co or immunized with SRBC) and on intact hybrids F1, CBAxC57BL. Phase contract microscopy showed autoimmune hemolysis plaques to be large or irregular and to posses a girdle made up of deformed and lysed erythrocytes and to contain stroma "ghosts" of the latter around the central cell. Plaques obtained in hypertensive medium were always round and the bordering girdle consisted of intact erythrocytes, whereas the plaque filed was homogeneous or contained granules. The concentration of sodium, potassium, calcium, and magnesium atoms in the suspension fluid of the preparations failed to exceed their content in the cells (as demonstrated by Jerne's method modified by the authors on a mixture of spleen cells with autologous blood). Addition of antiglobulin serum into the preparation on a slide in a 1:5 dilution suppressed the formation of autoimmune plaques, and in a dilution of 1:50--increased it considerably.

Animals↗
PubMed1978

[ Hoigne reaction and its modifications].

The author analyzes 114 works published lately dealing with methodical improvement of Hoigne's reaction and its application both in experimental studies and in clinical practice. The great majority of the works (over 90) were referred to the use of modification of this reaction suggested by Klemparskaya and Raeva for detection of autoantibodies to the lysate of autoerythrocytes and organ extracts. This reaction was found to be useful for studying the autosensitization process in man and animals; its prognostic significance at the clinic was of importance since its intensity corresponded to the severity of the process and promptly decreased with effective treatment.

Animals↗
PubMed1978

[Characteristics of early autoimmune reaction in vaccinated and revaccinated animals].

Experiments were conducted on albino mongrel female mice weighing 18 to 22 g. Development of early autoimmune reaction in response to the primary administration of microbial antigens was revealed; its intensity and duration depended on the dose of the antigen. Injection of physiological saline of the same or greater volume led only to the short-lived increase in the count of the autoantibody synthesizing cells in the spleen tissue. In repeated contact with the given antigen, there was noted a marked intensification of the reaction to homologous antigen, and a depression--to heterologous one in 7 to 14 days. Reaction to the administration of physiological saline alone failed to alter.

Animals↗
PubMed1978

[Specific immunologic reaction in autoantibody-producing cells].

Experiments were conducted on albino mongrel female mice. The capacity of the plasma obtained in 30 minutes from the animals vaccinated uith the microbial antigens of the animals to cause temporary activation of the autoimmune reaction in the recipients, in passive transfer, was revealed. The capacity of spleen cells, forming autohemolysins, to produce antibodies to sheep red blood cells after the immunization with this antigen was demonstrated by means of Cunningham and Pilarsky's method.

Animals↗
PubMed1977

[Vesiculocytosis].

Phenomenon of false plaque formation due to release of the cell contents into the surrounding environment and cell pyknosis occurred in case of replacement of physiological saline by hypertonic (10--15%) salt solutions in making the blood and tissue preparations for detection of plaque-forming cells producing autoa antibodies. This phenomenon took place with blood karyocytes of various species of animals and man with the action of different salts, for example sodium nitrate, ammonium sulphate, calcium chloride, etc. False plaque formation in hypertonic solutions failed to take place in all the cells in the preparation, i.e. depended on cell condition. This phenomenon was particularly pronounced at the period of maximal immune response of the cells.

Animals↗
PubMed1977

[Quantitative patterns in changes in the intestinal microflora of irradiated mice].

Experiments were conducted on 3 groups of mice, gamma-irradiated in doses of 900, 600 and 300 r. Development of acute radiation sickness was accompained by development of intestinal dysbacteriosis. Characteristic was reduction of lactobacilli content and increase in the content of purulent microbes (E. coli, clostridia, Proteus, and enterococcus) in the large intestine, and an increase of enterococcus and Proteus content in the small intestine. Changes in the microbial count in the intestine, depending on the time lapse after the irradiation can be represented as a formula of linear dependence; this permitted to compare the rate of changes in the intestinal content of the microbes during the development of acute radiation sickness.

Acute Disease↗
PubMed1976

[Theoretical and practical significance of the plaque formation intensification phenomenon in the presence of a specific antigen in experiments in vitro].

Experiments were conducted on 40 guinea pigs, 30 rats and 75 mice; a study was made of the presence and the mechanism of the phenomenon of intensification of plaque-formation under conditions of penicillin, ursol, horse serum, homologous intestine extract sensitization, or after hemostimulation on addition of a homologous antigen to preparations made by the modified Jerne's method. The presence of the phenomenon after the animal contact with penicillin, ursol, or hemo-stimulation, and its dependence on the interaction of the allergen with the immunocompetent cells were revealed.

Allergens↗