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N N Samsonova

Publications and source records attributed to N N Samsonova.

At least 19 recordsLinked to original sources
PubMed2006

[Modified capillary blood prothrombin-time test for the calculation of an international normalized ratio].

Prothrombin time (PT) is usually measured in citrated venous blood. This test is undesirable in pediatric practice and in patients taking anticoagulants due to frequent venepunctures. Capillary blood PT test has no adverse impact. The authors have developed a standardized modified method for PT measurement in capillary blood. The adequacy of the test is due to the fact that analyzed test packed cell volumes are kept in mind. The modified test has shown that the values of normal plasma PT may be used to calculate an international normalized ratio (INR). It makes it possible to determine capillary blood PT in the range of packed cell volumes from 0.15 to 0.7 in the examinees and to monitor anticoagulant therapy. The results of the modified test closely correlated with those when determining PT and INR in venous plasma (r = 0.99) and applying the CoaguChek test (r = 0.97).

Anticoagulants↗
PubMed2002

[Hematological characteristics in blood autoanalysis in children with congenital heart defects].

Hematological status of infants with congenital heart disease (CHD) was evaluated by automated systems. Blood analyses were carried out in 40 infants with the pale type and in 20 with the blue type CHD. Increased erythrocyte count and hematocrit were detected in the blue type CHD. Evaluation of the leukocytic formula showed correlation of the results of automated analysis and microscopic analysis of a blood smear. Automated method is preferable for estimating platelet count. Platelet count was decreased and the cells were larger in patients with the blue type disease in comparison with the pale type group. Hence, automated analyzer gives a wide spectrum of blood values with high accuracy and productivity and helps evaluate hemopoiesis in patients with CHD.

Autoanalysis↗
PubMed2002

[Hemorheology and morphofunctional characteristics of blood cells in children with congenital heart defects].

Patients with congenital heart diseases (CHD) aged 4 months to 12 years, hospitalized for surgical treatment, were examined. Complex viscosity of the blood and the constituents of this parameter (dynamic viscosity and elastic component) were evaluated in the range of shift strain corresponding to the range of shift velocities 0.37-500 s-1 at a frequency of 2 Hz. The threshold blood fluidity was estimated for evaluating the conditions under which erythrocyte disaggregation begins. The relationship between blood rheology and morphofunctional characteristics of blood cells was evaluated. In accordance with the findings, the patients were divided into 3 groups with different suspension stability of the blood. In group 1 the threshold fluidity approximated the norm. In group 2 with normal blood rheology the shifts were compensated and regulation of rheology was in general intact. In group 3 the hematocrit values, mean erythrocyte volume, mean concentration of hemoglobin in erythrocyte, leukocyte counts, and complex viscosity of the blood were the highest, while the suspension stability was the lowest, which indicates depletion of the adaptive potential of the organism. Hence, blood rheology in patients with CHD differs by the type of regulation, mechanisms and compensation of changes, and depend largely on the mean erythrocyte volume, mean hemoglobin concentration in erythrocytes, and leukocyte counts.

Blood Cell Count↗
PubMed1999

[Production of recombinant hIL-4delta2-a native isoform of human interleukin-4 in Escherichia coli cells].

Expression plasmids containing the synthetic gene hil-4 delta 2 was constructed to produce human interleukin-4 in Escherichia coli cells. Strains TG1 (pBTIL-4 delta 2) and BL21 (DE3) (pETIL-4 delta 2) produced the recombinant protein as inclusion bodies, and its production level was up to 30% of the total cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T cell proliferation, and this effect was enhanced by cyclosporin A.

Cell Division↗
PubMed1989

[Transfusion problems in artificial blood circulation].

Results of differentiated administration of blood components to cardiac surgical patients are presented. Frozen washed erythrocytes and erythromass were successively used in the perfusate and postoperatively. A transfusion pattern for patients with rare A2B blood group has been elaborated. The feasibility of prevention of artificial-circulation-induced thrombocytopenia by introduction of large quantities of platelet components right after AC is demonstrated. The necessity of the ACT-test for adequate blood heparinisation and neutralisation during the AC is established. Results of successful autohemotransfusion in cardiac surgery and application of fibrinolytic blood and its components are discussed.

Blood Transfusion↗
PubMed1983

[Role of immunologic reactions in the development, prevention and treatment of postoperative anemia].

On the basis of an analysis of immunological and hematological indices the authors have shown the role of activation of physiological hemolysis, immunization of the organism by erythrocyte antigens, inhibition of erythropoiesis due to intoxication and massive hemotransfusions in the development of early and late anemias after operations for congenital and acquired heart diseases under conditions of the artificial blood circulation. The authors propose recommendations for the prevention of such anemias.

Anemia, Hemolytic, Autoimmune↗
PubMedDate not supplied

[Inflammatory response mediators in newborns and infants with congenital heart diseases during surgical treatment under extracorporeal circulation].

Surgical treatment of complex congenital heart disease under extracorporeal circulation is accompanied by a systemic inflammatory reaction occurring in neonatal infants and babies. There were drastic increases in the concentration of tumor necrosis factor, interleukin-8, neutrophilic elastase and a predominance of a proinflammatory response over an inflammatory one at the warming stages and after administration of protamine sulfate. The rate of an inflammatory response depended on the duration of extracorporeal circulation. Modified ultrafiltration could remove cytokines; however, their concentration in the body remains high. A relationship was found between the course of a postoperative period and the rate of a developing reaction to extracorporeal circulation in the surgical treatment of congenital heart diseases.

Cardiac Surgical Procedures↗
PubMedDate not supplied

[Clinical experience with voluven solution used as a basic component for pump oxygenator filling during operations on the cardiac valves and coronary arteries].

The present study comparatively analyzed the clinical effects of three solutions used to fill an extracorporeal circulatory circuit (ECCC). The arterial and venous blood samples taken from patients operated on for valvular disease and coronary heart disease were studied. All patients (n = 61) were divided into 3 groups according to the basic component of the primary volume of ECCC filling: 1) Ringer's crystalloid solution; 2) gelofusin; 3) the hydroxyethyl starch 130/0.4 Voluven. The resultant samples were examined for the following parameters: a) hematological: the levels of formed blood elements, hemoglobin, erythrocytic hemoglobin, and free hemoglobin and the histograms of leukocytes, erythrocytes, and platelets; b) physicochemical: arterial and venous blood pH, pO2, pCO2, SatO2; c) biochemical: blood electrolytic balance and lactate levels. The values of hemoglobin, hematocrit, and blood gas composition were used to calculate oxygen consumption (ml/min x m2). The parameters of central hemodynamics and the temperatures in the rectum, esophagus, and venous oxygenator port were continuously monitored during extracorporeal circulation (EC). At its end, overall diuresis and water balance were determined. The plasma-substituting effect of Voluven was found to be highly effective during a clinical study. The found beneficial properties of the agent may be particularly attractive while applying EC to the surgery of acquired heat diseases when the duration of an operation and, accordingly, extracorporeal support forces the physician to administer large solution doses many times.

Acid-Base Equilibrium↗
PubMedDate not supplied

[An initial experience of extracorporeal irradiation of the blood in heart surgery patients].

Ultraviolet (UV) blood irradiation has been performed in 11 patients with postoperative sepsis. UV blood irradiation was not accompanied by blood cell damage and stress reaction to irradiation. Already after the first session all the patients demonstrated a decrease in the maximum daily body temperature. Most patients revealed improvement in their somatic condition. Positive clinical effect was observed in 8 patients, 4 patients recovered completely. UV blood irradiation in combination with conventional therapeutic techniques may be used in the management of postoperative septic complications in cardiosurgical patients.

Blood↗