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N Nakazono

Publications and source records attributed to N Nakazono.

52 records · Page 3Linked to original sources
PubMed1984

[Studies on rubella vaccination in adult females. I: Clinical and laboratory studies on clinical reactions].

More than 1,000 young females were screened for rubella HI antibodies. Clinico-laboratory studies were carried out in 272 seronegative healthy adult women who received Japanese licensed rubella vaccine, TO-336 or MEQ11 vaccine. Clinical reactions were observed in 51 (29.7%) of 172 vaccinees, aged 18-30 years (mean age; 20. 8 year), who received TO-336 vaccine, whereas only 4 cases (4.0%) had clinical reactions in 100 vaccinees, aged 18-26 years (mean age; 19.1 year), who received MEQ11 vaccine. The most troublesome and common clinical reaction was arthralgia, particularly in women received TO-336 vaccine. Joint symptoms, especially the knee, hand and finger joint, were observed in 41 of 51 cases with clinical reactions after TO-336 vaccination. On the other hand, vaccinees who received TO-336 vaccine gave a more satisfactory seroconversion response than vaccinees who received MEQ11 vaccine (100% vs 97%). This difference was also reflected in the geometric mean titers (1: 58 vs 1: 31). These results suggested strongly that MEQ11 vaccine might be a more attenuated rubella vaccine than TO-336 vaccine, according to the clinical reactions and antibody response. Associations of host factors to clinical reactions were examined. There was not a significant difference of antibody response between vaccinees who developed clinical reactions and those who did not. But vaccinees with HLA-B 15 responded well and high against two rubella vaccines. There was no specific association between HLA types and clinical reactions.

Adolescent↗
PubMed1984

[Studies on rubella vaccination in adult females. II: Hormonal influence on clinical reactions].

All rubella vaccines may, on occasion, cause mild and transient clinical reactions, especially joint symptoms, which occur most commonly in females with increase in frequency and severity with advancing age. These facts suggested the role of hormonal influences. To clarify the role of hormonal influences on the development of clinical reactions, 110 vaccinees, aged 18-30 years (mean age; 20.9 year), who received TO-336 vaccine were examined for their menstrual cycles and basal body temperature (BBT). Clinical reactions were noticed in 33 cases (30.0%), including 26 cases with joint symptoms, 12 cases with lymphadenopathy and 1 case with a rash. Clinical reactions, especially joint symptoms, were observed significantly more often in vaccinees when the vaccine was given at the high phase on BBT than in vaccinees when the vaccine was given at the low phase on BBT. Serum progesterones were radioimmunoassayed at an interval of 7 days for 6 weeks after vaccination in 88 vaccinees (80.0%) of 110. Clinical reactions, especially joint symptoms, occurred more commonly in vaccinees who received vaccine at the progestational stage than in vaccinees who received it at the estrogenic and menstrual stages. But there was not a significant difference of progesterone levels between the reaction group and non-reaction group who received vaccine at the progestational stage. These results mentioned above were confirmed at any age groups; 18-20, 21-25 and 26-30 year of age. But there was not a significant difference of antibody response by the stage when the vaccine was given. Initial clinical reaction occurred commonly at the menstrual stage. According to the periods of the hormonal stage, clinical reactions, especially joint symptoms, occurred more commonly at the menstrual stage, but least commonly at the progestational stage.

Adolescent↗
PubMed1983

Restriction endonuclease cleavage analysis of adenovirus type 8: two new subtypes from patients with epidemic keratoconjunctivitis in Sapporo, Japan.

We studied the restriction endonuclease cleavage patterns of DNAs of adenovirus type 8 (Ad 8) isolated from epidemic keratoconjunctivitis cases. DNAs of 25 Ad 8 isolates collected during the period from 1975 to 1981 in Sapporo were subjected to enzymatic cleavage with PstI, BamHl , HindIII and SalI. On the basis of the cleavage patterns, the isolates were divided into two subtypes, A and B, both different from Trim strain used as the Ad 8 prototype strain. Subtype A was prevalent in the period from 1975 to 1978, while subtype B in the period from 1976 to 1981.

Adenoviridae Infections↗
PubMed1982

Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus types 4 and 19 infections.

The clinical and aetiological findings are presented on 343 patients with clinical adenoviral conjunctivitis treated between July 1979 and July 1980 at 3 eye clinics in Sapporo. The age of the patients ranged from 12 days to 79 years, and the monthly incidence of the disease from 9 to 83, with clustering in the summer season. The aetiological diagnosis was established in 196 (57%) of 343 patients: adenovirus 3 in 24 cases; adenovirus 4 in 33 cases, including one case serologically diagnosed; adenovirus 8 in 124 cases; and adenovirus 19 in 15 cases. The different serotypes caused different clinical pictures. Adenovirus 19 conjunctivitis was more severe, with keratitis and preauricular lymphadenopathy more frequent than that of adenovirus 3 conjunctivitis. Adenovirus 4 conjunctivitis was generally similar to adenovirus 3 conjunctivitis. The clinical difference between the 2 groups adenoviruses 3 and 4, and adenoviruses 8 and 9, was statistically significant. Although in Japan adenovirus 8 was the most prevalent, adenoviruses 4 and 19 should be considered as causative agents of adenoviral conjunctivitis.

Adenoviridae Infections↗
PubMed1981

Host factors and susceptibility to rubella virus infection: the association of HLA antigens.

To examine the role of host-dependent factors to natural rubella infection, the positive rates of HAI antibody according to HLA-A and -B antigens were studied in 93 children, aged 9 to 10, who were exposed only to a recent epidemic (single-exposure group), and in 199 adult females, aged 18 to 23, who were exposed also to a former epidemic (dual-exposure group). Furthermore, the phenotype frequencies of HLA antigens were investigated in seronegative subjects in both groups, as well as in 58 adult female vaccinees, in comparison with those of the Japanese population. The seropositive rates by the HLA antigens, 38.7% and 73.4%, respectively, in each exposure group were found in subjects having some HLA antigens. Several of these candidate HLA antigens varied in phenotype frequencies in seronegative subjects of all three groups from those of the average Japanese. Although a few types were statistically significant, no contradictory findings in susceptibility were obtained among the groups during the study, which suggested strongly that subjects with HLA-Bw51 and -Bw16 are susceptible. In addition, when the seropositive rates were compared in all combinations of HLA-A and -B antigens classified according to expected susceptibility, the positive rates were arranged in order of susceptibility with significant statistical differences in several groups.

Adult↗
PubMed1981

Seroepidemiologic studies of acute hemorrhagic conjunctivitis virus (enterovirus type 70) in West Africa. II. Studies with human sera collected in West African countries other than Ghana.

Human sera were collected in Senegal, Nigeria, Ivory Coast, Dahomey, Liberia, Gabon and Togo during the pre-epidemic period of acute hemorrhagic conjunctivitis (AHC) from 1965 to 1969, and tested for virus neutralizing (VN) antibody to enterovirus type 70 (EV70). Of these, 1109 (91%) were antibody negative (less than equal to 1:4), 116 (9%) neutralized at a dilution of 1:8 or over, and 45 (4%) at dilutions of at least 1:16. The distribution pattern is not significantly different from that of sera collected from Kenya in 1967 or from army recruits in the United States, Argentina, Brazil and Colombia in the 1960s. Sera collected during the post-epidemic period (1970 to 1977) in Senegal, Sierra Leone, Mali, Upper Volta, Chad, Niger and Gabon were also examined; 1573 (68%) were VN antibody negative (less than or equal to 1:4), while 733 (32%) and 433 (19%) had titers of 1:8 or greater and 1:16 or over, respectively. There is a significant difference in distribution between pre- and post-epidemic antibody titers (p less than 0.001), although the incidence of AHC was lower in these countries than in Ghana and Southeast Asia. The prevalence of VN antibodies tends to be lower in the dry, hot inland areas and thus humid coastal monsoonal climates and dense populations seem to favor the spread of AHC.

Adolescent↗
PubMed1981

Seroepidemiologic studies of acute hemorrhagic conjunctivitis virus (enterovirus type 70) in West Africa. III. Studies with animal sera from Ghana and Senegal.

One hundred and thirty-nine bovine sera collected in Senegal in 1968 and 1969, before the human pandemic of acute hemorrhagic conjunctivitis (AHC), and 145 sera collected in 1977, seven years after the introduction of AHC, were tested for virus neutralizing (VN) titers against enterovirus 70 (EV70) in neutralization tests. Positive rates of VN titers (1:16) were fairly constant (about 40%) in 1968 and 1969 but the proportion of positives from the 1977 collection was significantly higher (61%). The proportion of Ghanaian bovine sera positive in 1977 (39%) was comparable with those collected earlier in Senegal but the proportion was lower in sera of calves (7%). Similar studies were performed on sheep sera collected in 1966, 1967, 1968, and 1969 in Senegal. The proportions positive and the geometric mean titers (GMTs) peaked in alternate years: the proportions were 43% and 54% in 1966 and 1968 but dropped to 12% and 5% in 1967 and 1969, respectively. The prevalence rate for Ghanaian sheep sera in 1977 was comparable to the earlier figures in Senegal. The prevalence of VN (83%) and the GMT (1:27.28) in swine sera in Ghana in 1977 were much higher than those seen in chickens and dogs. However, 87 sera of wild monkeys caught in Senegal after 1970 were negative in neutralization tests. The VN substance detected in these domestic animals was found to be 2-mercaptoethanol sensitive; hence it is considered to belong to IgM. The results seem to favor the hypothesis that enterovirus type 70 (EV70) evolved from an animal enterovirus which shares a common antigen with EV70.

Animals↗
PubMed1980

Antibody response following rubella immunization analyzed by HLA types.

Possible association between HLA antigens and antibody response to rubella vaccine was examined in 71 seronegative adult females, aged from 18 to 23, immunized with QEF vaccine. High responders (HAI antibody titer greater than or equal to 1:32) with HLA-B15 were significantly higher in frequency than those without HLA-B15 (p < 0.05). On the other hand, subjects with HLA-A9 had a tendency toward low immune responsiveness, including all the three non-responders. These results suggest that the antibody response to rubella vaccine may be influenced by the host-genetic factors relating to HLA antigens.

Adolescent↗
PubMed1975

[Spread of acute haemorrhagic conjunctivitis virus in virgin soil (Hokkaido, Japan): observation with serological surveys (author's transl)].

Acute haemorrhagic conjunctivitis (AHC), a novel disease caused with a new type of enterovirus, occurred for the first time in Sapporo City and other areas of Hokkaido in October of 1971. During first 4 months, 1,000 or more cases of AHC were reported in Sapporo and 2,300 cases in other areas. Thereafter, there are no epidemic of AHC in any areas, though there are sporadic cases up to date. To know the spread of exogenous agent, AHC virus, in Hokkaido, the neutralizing antibody was measured with sera of healthy adults resident in 12 separated districts of Hokkaido in 1973 and 1974. It was revealed that the positive reactors to AHC virus were found in 5 to 35% in all districts but one in 1974, and AHC virus was distributed in almost all the areas of Hokkaido within a period of two and half years after invasion. In addition, a sequential sero-survey to detect the spread of AHC virus has been carried out in Sapporo. A rate of neutralizing antibody positives in healthy adults did not show a clear increase on sera withdrawn one year after epidemic of AHC in comparison with those before epidemic, but show a remarkable increase on sera withdrawn later in spite of non-epidemic. An increase of antibody positive rate in non-epidemic period was also obtained from sera of healthy adults resident in districts other than Sapporo. These findings made doubt the changes in transmission on mode and/or in clinical pictures, and the viral mutation in biological character, such as temperature for propagation, may be supposed.

Adolescent↗
PubMedDate not supplied

A simple, rapid, highly sensitive and reproducible quantification method for plasma malondialdehyde by high-performance liquid chromatography.

A simple, rapid, highly sensitive and reproducible quantification method for plasma malondialdehyde (MDA) was developed using high-performance liquid chromatography (HPLC). The present method is based on the thiobarbituric acid (TBA) reaction and reversed-phase HPLC separation with fluorescence detection. For sample preparation, an aliquot of 5 microL plasma was mixed with 0.2% (w/v) TBA in 0.1 M sodium acetate buffer, pH3.5. After heating at 95 degrees C for 60 min, the reaction solution was centrifuged and an aliquot of 10 microL supernatant was injected into the HPLC system without neutralization or extraction procedures. The TBA-MDA adduct was separated on a reversed-phase column and quantified by a fluorescence detection (lambda ex = 515 nm; lambda em = 553 nm). The mobile phase was a mixture of acetonitrile: water (7:3, v/v) under isocratic conditions at ambient temperature. These procedures gave good results with regard to sensitivity (detection limit S/N = 3, 0.5 fmol per injection), linearity (0.5-500 fmol per injection), precision (between-assay C.V = 3.1%, within-assay C.V = 1.2%) and recovery (98.6%). The simple sample procedure, the highly sensitive detection limit and the stability of the TBA-MDA adduct make the present method applicable to numerous clinical samples. As many as 250-300 samples per day can be assayed using an autosampler.

Adult↗