[Changes of the CD69 expression and cytokine secretion in confined environment].
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Biomedical subjects
Publications and source records attributed to N Naraki.
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The sleep patterns were examined during the simulated 30-m nitrox saturation dives. The standard polysomnography of 15 divers was recorded for a total of 255 nights, as were patterns of change or consistency in sleep variables. A reduction of total sleep time in accordance with the lengthening of sleep latency and the wake after sleep onset was observed through the latter part of the bottom period to the post-dive period, but the other sleep variables did not show any changes. These findings suggest that decompression and the psychological stress due to being in the closed environment of a hyperbaric chamber for a long time have effects on divers' sleep.
The isolated and confined environment of a laboratory setting was used as one of the analogous environments of long-term manned spaceflight. This setting has more advantages than the natural one in that the behavioral variables can be easily controlled. Thus, a hyperbaric chamber was used in this experiment. The subjects were five men from 22- to 26-years old who had never met before the experiment. Over a total 5-day isolation and confinement period as well as 2-day pre- and post-isolation periods, the subjects were given different kinds of physiological and psychological tasks. During these periods their behavior and communications were recorded in order to observe when the formation of a group begins and how it forms and functions. The formation of a sub-group of three men was observed on the third day of isolation, showing strong reciprocal connections among these subjects who developed bonds that were quite stable. It is, however, not known how this sub-group functioned within the large group, since the experimental period was possibly too short.
The purpose of the present study is to evaluate the static work load as a model work in Submersible Decompression Chamber (SDC) during a deep sea saturation dive with helium-oxygen gas mixture. Heart rate (HR) and electromyogram (EMG) power spectrum changes were studied during a 7-minute static work, half-rising posture or flexed knee posture in four healthy male subjects. During the static work, EMG of the rectus femoris was recorded with surface electrodes, and changes in the EMG power spectrum were presented in the ratio of a high-frequency to low-frequency band (H/L ratio) after the Fourier transform analysis. The HR decreased at 31 ATA and increased remarkably after the decompression to 1 ATA. HR at post-decompression was higher than pre-compression. The lowering phenomena of EMG presented by H/L ratios during the static work were similarly observed in all three conditions. But the changes of the high and low frequency components were different in the post-decompression condition from the pre-compression and 31 ATA conditions. HR as a parameter of static work load might underestimate the work load at the hyperbaric environment due to hyperbaric bradycardia and overestimate it after decompression by "decompression tachycardia." The EMG lowering phenomenon observed after decompression might not be caused by the same mechanism as seen in the pre-compression and hyperbaric environments. Extreme care must be taken to evaluate the static work load not only at hyperbaric helium-oxygen environments but after decompression from a deep saturation dive.
We examined the upper limit of a diver's fin-kick thrust force using a stationary-swimming ergometer. Heart rate, respiratory minute volume, oxygen uptake, and performance rate were measured in four male subjects who swam constantly for 8 min to maintain a horizontal position against an applied force at a depth of 0.7 m. The water temperature was controlled at 26 degrees +/- 1 degree C. The performance rate, which was the parameter of how well the subjects compensated for the applied load, showed an upper limit around 64 N of sustainable thrust force. This meant that the diver could generate the swimming thrust force within 64 N continuously for 8 min in a steady state. Heart rate, respiratory minute volume, and O2 uptake showed almost proportional increases to the applied load within 64 N and tended to plateau about 69 N.
Ten adult, awake cats were exposed to high pressure (7.5, 9, or 10 MPa) of a heliox (He-O2) or trimix (He-N2-O2) gas mixture. Total duration of the experiment, i.e., duration of compression plus sojourn at maximal pressure, varied between 23 and 59 h. Throughout the experiment, minute ventilation (VE), heart rate, and rectal temperature were recorded. The total mass of gas (Mt) breathed by each animal was determined from the product of VE, gas density (p), and time (t). As previously shown, VE was increased in all animals breathing heliox mixtures, whereas this was never observed in trimix experiments. Ventilatory arrest occurred before cardiac failure in 3 animals breathing heliox mixtures, where the highest values of Mt were measured; the others survived. Rectal temperature, t, or p values did not account for the difference between those animals surviving and those who died. Thus, increased ventilation in high density gas mixtures was responsible for increased values of Mt. Present observations suggest that ventilatory failure associated to the highest values of Mt is related to respiratory muscle fatigue.
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The effect of pressured environment from 1 to 24 ATA (Atmosphere Absolute) on total power spectrum (TP) of physiological tremor, whose value was the sum of power spectra for frequency range in 0.5-50 Hz, was investigated. The main effects obtained were as follows. (1) In the case of 3 ATA, TP during the pressure holding period denoted a similar value as the value in 1 ATA. In the latter half of the period, the TP increased. When the pressured environment is 4 ATA, in which the partial pressure of the nitrogen gas was 3.6 ATA, the TP decreased during the pressure holding period compared with the value in 1 ATA. Nitrogen narcosis was recognized at the partial pressure of 3.6 ATA, thus the effect denoted a decrease of TP. (2) In the cases of 16 and 19 ATA using heliox gas, during the pressure holding period, TP decreased compared with the value in 1 ATA, but in the case of 24 ATA the value increased. The partial pressure of helium gas of 23.6 ATA indicated a high pressure nervous syndrome, therefore, the influence of high pressure on TP was recognized as one of the causes of the increase of TP. (3) The influence of inhibitors of the autonomic nervous system on TP during the pressure holding period of 3 ATA was recognized. After intake of the inhibitor for the parasympathetic nerve (atropine) during the pressure period, TP increased, while for the intake of the inhibitor for the sympathetic nerve (propanol), TP decreased.