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Biomedical subjects

N Nitta

Publications and source records attributed to N Nitta.

At least 19 recordsLinked to original sources

The Effect of Hydrostatic Weighting on the Vertical Temperature Structure of the Solar Corona.

We investigate the effect of hydrostatic scale heights lambda(T) in coronal loops on the determination of the vertical temperature structure T&parl0;h&parr0; of the solar corona. Every method that determines an average temperature at a particular line of sight from optically thin emission (e.g., in EUV or soft X-ray wavelengths) of a mutlitemperature plasma is subject to the emission measure-weighted contributions dEM&parl0;T&parr0;&solm0;dT from different temperatures. Because most of the coronal structures (along open or closed field lines) are close to hydrostatic equilibrium, the hydrostatic temperature scale height introduces a height-dependent weighting function that causes a systematic bias in the determination of the temperature structure T&parl0;h&parr0; as function of altitude h. The net effect is that the averaged temperature seems to increase with altitude, dT&parl0;h&parr0;&solm0;dh>0, even if every coronal loop (of a multitemperature ensemble) is isothermal in itself. We simulate this effect with differential emission measure distributions observed by SERTS for an instrument with a broadband temperature filter such as Yohkoh/Soft X-Ray Telescope and find that the apparent temperature increase due to hydrostatic weighting is of order DeltaT approximately T0h&solm0;r middle dot in circle. We suggest that this effect largely explains the systematic temperature increase in the upper corona reported in recent studies (e.g., by Sturrock et al., Wheatland et al., or Priest et al.), rather than being an intrinsic signature of a coronal heating mechanism.

Journal Article↗

Pattern recognition as a caring partnership in families with cancer.

Pattern recognition as a caring partnership in families with cancer The purpose of this study was to address the process of a caring partnership by elaborating pattern recognition as nursing intervention with families with cancer. It is based on Newman's theory of health as expanding consciousness within the unitary-transformative paradigm and is an extension of a previous study of Japanese women with ovarian cancer. A hermeneutic, dialectic method was used to engage 10 Japanese families in which the wife-mothers were hospitalized because of cancer diagnosis. The family included at least the woman with cancer and her primary caregiver. Each of four nurse-researchers entered into partnership with a different family and conducted three interviews with each family. The participants were asked to describe the meaningful persons and events in their family history. The family's story was transmuted into a diagram of sequential patterns of interactional configurations and shared with the family at the second meeting. Evidence of pattern recognition and insight into the meaning of the family pattern were identified further in the remaining meetings. The data revealed five dimensions of a transformative process. Most families found meaning in their patterns and made a shift from separated individuals within the family to trustful caring relationships. One-third of them went through this process within two interviews. The families showed increasing openness, connectedness and trustfulness in caring relationships. In partnership with the family, each nurse-researcher grasped the pattern of the family as a whole and experienced the meaning of caring. Pattern recognition as nursing intervention was a meaning-making transforming process in the family-nurse partnership.

Adaptation, Psychological↗

A retrospective study of speech development in patients with submucous cleft palate treated by four operations.

Fifty patients with submucous cleft palate (SMCP) who had had four different operations were reviewed. The operations were pushback palatoplasty (n = 18), pharyngeal flap (n = 21), pushback palatoplasty combined with a pharyngeal flap (n = 8), and Furlow palatoplasty (n = 3). Postoperatively the speech of 8, 19, 7, and 2 patients, respectively, improved so that it was within normal limits. A secondary pharyngeal flap was done for six patients, each of whom had previously had a pushback palatoplasty. They all improved, five achieving relatively normal speech, and one good speech. No patient developed hyponasality or airway compromise associated with the pharyngeal flap. The results show that pharyngeal flap and pushback palatoplasty combined with a pharyngeal flap seem to be more reliable procedures than pushback palatoplasty for patients with SMCP.

Child↗

Relation between Flare-associated X-Ray Ejections and Coronal Mass Ejections.

In an attempt to identify the direct signatures of coronal mass ejections (CMEs) in soft X-ray wavelengths, we have searched for plasma ejections in Yohkoh soft X-ray telescope (SXT) images in a total of 17 limb flares, and compared the results with the Solar and Heliospheric Observatory LASCO data. A general correlation exists between the presence/absence of the X-ray ejection and the CME. Although the height versus time relation often indicates (under the assumption of constant speed) that the CME onset coincides with the X-ray ejection, the latter probably does not represent the CME front, because the CME speed must result from acceleration, which would put the estimated onset at an earlier time. In some cases, the estimated CME onset time comes well before the impulsive phase of the associated flare. Although the role of the flare-associated plasma ejection in a CME is still unclear, we propose that its occurrence depends on the presence of open field lines, which can be due to a preceding CME. Lastly, we present a rare example of SXT observations of what appeared to be the three-part structure of a CME, which was seen a few minutes before a major flare started.

Journal Article↗

Hilar and mediastinal invasion of bronchogenic carcinoma: evaluation by thin-section electron-beam computed tomography.

The diagnostic accuracy of thin-section incremental dynamic computed tomography (IDCT) using an electron-beam scanner in evaluating hilar or mediastinal invasion of bronchogenic carcinoma was assessed. Thirty-seven patients with proven bronchogenic carcinoma, contiguous with hilar or mediastinal structures, underwent IDCT. The area of contact was scanned using 19 contiguous 3-mm thick sections during injection of contrast material. The degree of contact between mass and pulmonary artery or vein and their distortion were recorded. Irregular thickening of the bronchial wall, soft tissue within the lumen, or distortion were used to determine airway involvement. Sixty-nine sites were assessed retrospectively and compared with pathology reports. The accuracy, sensitivity, and specificity in evaluating invasion of the pulmonary artery were 75.0%, 77.8%, and 71.4%, respectively. Limited reliability also was found for invasion of the main bronchus and "secondary" carina, with accuracies of 66.7% and 70.5%, sensitivities of 75.0% and 70.0%, and specificities of 57.1% and 71.4%, respectively. Thin-section IDCT with electron beam scanner is not accurate in the detection of hilar or mediastinal invasion by bronchogenic carcinoma.

Adult↗

Craniofacial morphology of conotruncal anomaly face syndrome.

OBJECTIVE AND DESIGN: The conotruncal anomaly face syndrome (CTAF) comprises congenital heart disease and dysmorphic face, and is frequently associated with cleft palate or hypernasality. There have been many discussions about the overlap with velocardiofacial syndrome (VCF). The aim of this study was to clarify the craniofacial characteristics of CTAF patients by clinical examination, and photogrammetric and cephalometric analyses, and to clarify the differences compared to published data on VCF. RESULTS: The facial features of CTAF included hypertelorism, small palpebral fissures, upward slanting of palpebral fissures, bloated eye lids, low nasal bridge, small mouth, open mouth at rest, and malformed auricles. Cephalometric features included bialveolar protrusion, small gonial angle, backward rotation of the mandibular ramus, and labial inclination of the maxillary incisors. An acute cranial base angle was also noted. These results differed from those of VCF. There were, however, no obvious pathognomonic findings for the differential diagnosis between CTAF and VCF. CONCLUSIONS: Considering these findings, use of CATCH 22, the inclusive classification of cardiac anomalies, cleft palate, and dysmorphic face may be of value for the clinical understanding in these patients.

Alveolar Process↗

Double cystic duct found by intraoperative cholangiography in laparoscopic cholecystectomy.

We report a rare case of double cystic duct in a 74-year-old woman. The patient complained of mild epigastric discomfort, and several stones were discovered by ultrasonography and computed tomography. Any anomalies of the biliary tract were undetectable in the preoperative examinations without direct cholangiography. Laparoscopic cholecystectomy was performed. After clipping the cystic duct close to the gallbladder, as usual, serial intraoperative cholangiography was performed and unexpectedly showed the inflow of contrast medium into the gallbladder via another cystic duct arising from the right hepatic duct, thus revealing one gallbladder and two cystic ducts, one of which joined the common hepatic duct and the other the right hepatic duct. There was only one cystic artery that arose from the right hepatic artery and accompanied the primary cystic duct to be distributed to the gallbladder. The existence of contrast medium in the resected specimen was confirmed by radiography. No complications occurred during or after laparoscopic cholecystectomy. This is the first report of double cystic duct found in laparoscopic cholecystectomy. We recommend routine preoperative or intraoperative cholangiography.

Aged↗

[A new mechanically manipulated unit for CT-guided biopsy].

To avoid radiation exposure to the radiologist during biopsy with CT fluoroscopy, we have developed a new system for CT-guided biopsy. The system is composed of three parts, a needle holder, arm and support. The automatic biopsy needle is mechanically remote controlled by flexible shafts with four functions. In the phantom study and the clinical trial, we were able to biopsy nodules under remote control with this equipment, eliminating radiation exposure to the radiologist. Although a few refinements of the system are necessary, it may enable us to perform CT-guided biopsy more safely and easily.

Animals↗

MAGGY, a retrotransposon in the genome of the rice blast fungus Magnaporthe grisea.

Full-length copies of a previously described repetitive DNA sequence (CH2-8) were isolated from the genome of the Magnaporthe grisea strain 2539. One copy of the complete element was sequenced and found to resemble a gypsy-like LTR retrotransposon. We named this element MAGGY (MAGnaporthe GYpsy-like element). MAGGY contains two internal ORFs putatively encoding Gag, Pol and Env-like proteins which are similar to peptides encoded by retroelements identified in other filamentous fungi. MAGGY was found to be widely distributed among M. grisea isolates from geographically dispersed locations and different hosts. It was present in high copy number in the genomes of all nine rice-pathogenic isolates examined. By contrast, M. grisea strains isolated from other Gramineae were found to possess varying copy numbers of MAGGY and in some cases the element was completely absent. The wide distribution of MAGGY suggests that this element invaded the genome of M. grisea prior to the evolution of rice-specific form(s). It may since have been horizontally transmitted to other sub-specific groups. One copy of MAGGY, corresponding to the element we sequenced, was located at identical locations in the genomes of geographically dispersed strains, suggesting that this copy of the element is a relatively ancient insertion.

Amino Acid Sequence↗

Peribronchovascular interstitium of the pulmonary hilum: normal and abnormal findings on thin-section electron-beam CT.

The peribronchovascular interstitium, including bronchial vessels and lymphatic channels, is an important anatomic component of the lung, especially the pulmonary hilum [1-3] (Fig.1). Various pathologic processes involve and spread along this interstitium [4, 5]. Although it has been difficult to accurately evaluate the morphologic changes occurring in the peribronchovascular interstitium with conventional CT or MR imaging [6], spiral CT and electron-beam CT can show these changes clearly and consistently [7, 8]. A judgement as to whether the peribronchovascular interstitium is normal or abnormal is necessary for the CT diagnosis of pulmonary hilar lesions. In this assay, the normal peribronchovascular interstitium and various pathologic processes involving this interstitium are shown as they appear on thin-section electron-beam CT scans.

Aged↗

[Ultra-low-dose spiral (helical) CT of the thorax: a filtering technique].

To reduce the radiation dose from spiral (helical) CT, a custom-made aluminium filter was installed in the X-ray tube and a reduction of effective tube current was attempted. A pronounced reduction of effective tube current, namely, 6 and 3 mA, was achieved with 26 and 37 mm thick aluminium filters, respectively. Visualization of normal lung structure was accomplished with both 6 and 3 mA settings. However, images of 3 mA failed to delineate mediastinal structures because of marked beam hardening resulting from the bone structure of the thoracic inlet. Six mA was considered the lowest dose setting of spiral (helical) CT of the thorax that could be used for lung cancer screening.

Adult↗

[Anaphylactoid purpura: intestinal ultrasonographic findings].

We evaluated six children with anaphylactoid purpura for ultrasonographic changes in the small intestine. Of 16 ultrasonographic scans, eight showed abnormal findings, consisting of circumscribed hypo-echoic enlargement, reduced peristalsis, equivocal structure of the small intestinal wall and ascites in patients with abdominal pain. Follow-up ultrasonography after steroid therapy demonstrated the small intestine to be normal in four of six patients. We believe that ultrasonography is an useful diagnostic modality to detect changes in the small intestinal wall in anaphylactoid purpura and plays an important role in its differentiation from other causes of abdominal pain.

Adolescent↗

The pattern of palatal rugae in submucous cleft palates and isolated cleft palates.

The early diagnosis of submucous cleft palate is important. In children too young to tolerate nasendoscopy and videofluoroscopy the diagnosis depends on the clinical history and intraoral examination. We have studied the pattern of the hard palate rugae to investigate their possible diagnostic significance. Maxillary dental casts were obtained from 16 patients with submucous cleft palate, 17 patients with isolated clefts of the secondary palate, and 10 non-cleft controls. The hard palate mucosa had a unique feature in 14 (87.5%) of the submucous cleft palates: one or more of the palate rugae curved towards the region of the bony notch in the posterior border of the hard palate. The 2 cases without this rugae pattern did not have a detectable bony notch. In 100% of the isolated cleft palate cases, one or more of the rugae curved towards the anterior end of the cleft. This feature was not seen in any of the non-cleft controls. We consider this rugae pattern to be an additional diagnostic feature of submucous cleft palate.

Child↗

Blockage of polymerase-catalyzed DNA chain elongation by chemically modified cytosine residues in templates and the release of blockage for readthrough.

The Klenow fragment-mediated in vitro DNA elongation was inhibited by the presence of a class of modified cytosines in the template DNA, i.e., the N4-amino(and -methoxy)-5,6-dihydrocytosine-6-sulfonate residues. We have studied the mechanism of the blockage, using as templates bisulfite-hydrazine (and -methoxyamine)- modified single strand phage-M13mp2 DNA and synthetic oligonucleotides. Both N4-amino-5,6-dihydrocytosine-6-sulfonate and N4-methoxy-5,6-dihydrocytosine-6-sulfonate residues blocked the elongation at one nucleotide before these sites. In this blockage, the idling of polymerase at the lesion site due to its 3'-5' exonuclease action appears not to play a major role, because Sequenase that lacks the 3'-5' exonuclease activity still could not readthrough these sites. It seems possible that conformational distortion of the template near these sites is responsible for the blockage, because on conversion of this 5,6-dihydropyrimidine-6-sulfonate structure into a planar pyrimidine, a complete restoration of polymerase-readthrough resulted. In the presence of RecA and SSB proteins, the Klenow fragment was able to partially readthrough these sites. Since there was no decrease in the 3'-5' exonuclease activity during this readthrough, it seems that the binding of these proteins relaxes the distortion in the modified template to allow the polymerase to readthrough the lesion site. These sites on phage DNA can be lethal but also are capable of inducing C-to-T transitions. This observation suggests that these sites can be read by E. coli DNA polymerases in vivo with accompanying errors.

Bacteriophages↗

Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system.

Full-length DNA copies of RNAs 1, 2, and 3 of CMV Y strain (CMV-Y) were cloned downstream of modified phage T7 promoter sequences to obtain infectious RNA transcripts. The small number of extra nonviral nucleotides at the 5' ends considerably decreased the specific infectivity of the transcripts of RNAs 1 and 2 but did not affect that of the RNA3 transcripts. Using the most infective transcripts, up to 45% of tobacco protoplasts could be infected. Various cDNA mutants were constructed from the full-length RNA3 cDNA to give RNA transcripts having deletions in the coding region of the 3a protein or the coat protein. These mutants replicated in tobacco protoplasts but did not produce systemic symptoms on tobacco when inoculated together with transcripts of RNAs 1 and 2. One of the mutants having a small in-frame deletion near the N-terminal region of the coat protein produced local lesions on cowpea and local chlorotic spots on the inoculated leaves of tobacco. These results suggest that both the 3a protein and the coat protein are involved in virus transport, and that viral assembly is associated with long-distance movement of CMV.

Base Sequence↗

Effects of partial deprivation of portal blood on arterial blood ketone body ratio in rabbits.

To examine the effects of portal blood deprivation on energy metabolism of the liver, we studied: (1) the ketone body ratio (acetoacetate/beta-hydroxybutyrate) in liver tissue, which is in equilibrium with the free NAD+/NADH ratio in liver mitochondria, in the ligated lobe (LL) and nonligated lobe (NLL), (2) the hepatic energy charge [EC = (ATP + 1/2 ADP)/(ATP + ADP + AMP)] in both LL and NLL, and (3) the arterial blood ketone body ratio (BKBR) after left portal vein branch ligation (PBL) in rabbits. As found in LL after PBL, portal blood deprivation decreased the tissue ketone body ratio. The EC in LL significantly decreased after PBL, but recovered 7 days after PBL since the LL became atrophic. The BKBR remained within the normal range, even when 60% of the total liver was deprived of portal blood.

Animals↗