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N Ohwada

Publications and source records attributed to N Ohwada.

14 recordsLinked to original sources
PubMed2000

Identification of a 195 kDa protein in the striated rootlet: its expression in ciliated and ciliogenic cells.

Little is known about the molecular composition of the ciliary rootlet. We raised monoclonal antibodies to a crude preparation of striated rootlets isolated from the human oviduct, and obtained a clone (R4109) that specifically labeled the ciliary rootlets. Rootlets associated with the solitary cilium in secretory cells and fibroblasts were also labeled. R4109 identified a 195-kDa protein by immunoblotting. Ciliogenic cells in the oviduct epithelium of young mice were labeled in the globular and/or granular pattern by R4109 by immunofluorescence microscopy. Immunoelectron microscopy showed that they corresponded to fibrogranular complex and dense granule, respectively. The result demonstrated that the 195-kDa protein is a component common to the striated rootlet and dense granule, and thus suggested that dense granules are involved in the rootlet formation.

Animals↗
PubMed2000

Immunocytochemistry of the striated rootlets associated with solitary cilia in human oviductal secretory cells.

The human oviduct epithelium is a simple columnar structure that consists primarily of ciliated and secretory cells. Solitary cilia usually extend from the apical cell surface of secretory cells. By injecting crude preparations of striated rootlets into rats, we successfully obtained six monoclonal antibodies (R38, R67, R95, R149, R155, R213) that commonly labeled ciliary rootlets. Using these antibodies, proteins of 205-215 kDa were identified by immunoblotting. Using a clone, R67, we investigated the morphology of the striated rootlets associated with solitary cilia by immunocytochemistry. It was found that the shapes of the rootlets were not simple but varied considerably. The rootlets had branched, radiated, arched, and looped shapes. This is the first report of the rootlets having a variety of shapes. The 205- to 215-kDa antigens identified by the six different antibodies were mostly localized to dark bands of striations, suggesting that they are constitutive components of dark striations of the rootlet.

Animals↗
PubMed2000

Ciliogenesis and ciliary abnormalities.

Cilia are motile processes extending from the basal bodies, playing important roles in the mucociliary clearance in the respiratory tract and the transport of the ovum from the ovary to the uterus in mammals. Ciliogenesis is divided into four stages: (1) duplication of centrioles; (2) migration of centrioles to the apical cell surface to become basal bodies; (3) elongation of cilia containing the axoneme; and (4) formation of accessory structures of basal bodies. The orderly course of ciliogenesis appears to be disturbed by various internal and external factors and, as a result, various unusual forms of the ciliary apparatus develop in the cell. Inhibition of basal body migration results in development of intracytoplasmic axonemes, cilia within periciliary sheaths, and intracellular ciliated cysts. Swollen cilia and the bulging type of compound cilia are formed during ciliary budding and elongation. This review also discusses the origin, composition, and function of the centriolar precursor structures.

Animals↗
PubMed2000

Localization of gamma-tubulin to the basal foot associated with the basal body extending a cilium.

The human oviduct epithelium primarily consists of ciliated cells and secretory cells. Solitary cilia usually extend from the apical surface of the secretory cells. We investigated the localization of gamma-tubulin in the ciliary basal apparatus of both cell types by fluorescence immunohistochemistry and immunoelectron microscopy. In addition to basal bodies, gamma-tubulin was identified in the lateral basal foot, especially the basal foot cap. This observation is consistent with previous observations that microtubules radiate from the basal foot and the basal foot serves as the microtubule organizing centre.

Cilia↗
PubMed1998

Langerhans cells in the human oviduct mucosa.

Langerhans cells (LCs) are the predominant antigen-presenting cells in epithelial tissues. They have been known to be present in the vagina and uterine cervix. In the present study, localization of LCs in the oviduct was investigated by electron microscopy and immunohistochemistry using an anti-CD1a (CD; cluster of differentiation) antibody. Although the cell density was variable, CD1a-positive LCs were detected in the oviduct epithelium. Their occurrence was most common in women at the age of 40 to 59. LCs extended cell processes along the base of the epithelium and were ultrastructurally characterized by rod-shaped Birbeck granules and a well-developed Golgi apparatus. LCs, together with lymphocytes and macrophages, are considered to engage in the mucosal immune system of the oviduct.

Adult↗
PubMed1997

Development of striated rootlets during ciliogenesis in the human oviduct epithelium.

Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the same time as ciliary budding. After the formation of several striations, the tip of the rootlets extended deeply toward the interior of the cell and became differentiated into two distinct parts, viz., the proximal conical part connected to the basal body and the distal fibrillar part. The periodicity of the striations in the fibrillar part was 68. 5+/-2.95 nm, about 5 nm longer than that of the conical part (63. 9+/-2.25 nm). The dark band in the striation was thicker in the fibrillar part than in the conical part. Since the fibrillar part was not observed in the mature cilium, this part was considered as being either degraded or changed into the conical part during ciliogenesis.

Adult↗
PubMed1992

[Maintenance chemotherapy using UFT and FT for gynecological malignant tumors. The Gunma Prefectural Society for Study of Chemotherapy for Gynecological Cancer].

The Gunma Prefectural Society for Study of Chemotherapy for Gynecological Cancer made a comparative study on postoperative supplementary chemotherapy including UFT and FT-E for gynecological malignant tumors. The number of cases registered totaled 182, which was broken down into ovarian cancer 53 cases, uterine cervical cancer 89 cases and endometrial cancer 30 cases. Complete cases among them consisted of ovarian cancer 53 cases, uterine cervical cancer 86 cases and endometrial cancer 27 cases for a total of 166 cases. Ovarian cancer was stratified into the completely and incompletely removed cases, uterine cervical cancer into postoperative non-irradiated and irradiated cases and endometrial cancer into the positive metastasis (+) group and negative metastasis (-) group. Then the UFT administration group and FT-E administration group were compared for postoperative survival. There were control groups for both ovarian and endometrial cancer. UFT administration showed a significant improvement in the survival rate (P < 0.05) compared to the FT-E administration group in the cases of ovarian cancer completely removed and the cases of endometrial cancer irradiated after operation. No significant difference was observed between other groups compared. The incidence of side effects was 20.6% (20/97) in the UFT administration group and 3.6% (2/55) in the FT-E group. Neither group showed any serious side effects.

Adult↗
PubMed1992

Ciliogenesis in the human oviduct epithelium during the normal menstrual cycle.

Ciliogenesis has been investigated in the human oviduct epithelium during the normal menstrual cycle. Both centriolar and acentriolar pathways were involved in the replication of basal bodies. The centriolar pathway, in which procentrioles generate with the aid of preexisting diplosomes, played a minor role in the human oviduct. In the acentriolar pathway, fibrous granules were the first structure which appeared in the course of ciliogenesis and they initially occurred in association with the Golgi apparatus or free ribosomes. Subsequently deuterosomes arose in the aggregates of fibrous granules or apart from fibrous granules, and then microtubules-containing procentrioles originated around deuterosomes. Newly formed centrioles migrated to the apical cytoplasm with accompanying deuterosomes, and ciliary shafts extended first at the periphery of the luminal surface of ciliogenic cells. Deuterosomes as well as fibrous granules were considered to be related to the rootlet formation. Replicaion of basal bodies and protrusion of ciliary shafts mostly occurred during the proliferative phase of the menstrual cycle; however, a small number of fibrous granules indicating the ciliogenesis were still observed in some ciliated cells during the secretory phase. Ciliogenic cells in early stages of ciliogenesis contained secretory granules-like vesicles in the apical cytoplasm, suggesting that the ciliated cells are differentiated from secretory cells in the late secretory phase on demand.

Adult↗
PubMed1991

[Incidence of Chlamydia trachomatis isolated from endocervical columnar cells of the uterine cervix].

To study the incidence of Chlamydia trachomatis (C.T.) infection among women, we obtained swab (endocervical columnar cells) from the uterine cervix of 1,041 women attending the Department of Obstetrics and Gynecology of Gunma University Hospital. The results were as follows. 1) Overall, C.T. was isolated from 60 of the 1,041 women (5.8%). With reference to the diagnoses, the organism was isolated from 38 of 183 patients with cervicitis (20.8%) and from 22 of 858 asymptomatic womens (2.6%) (p less than 0.001). 2) C.T. antigen was detected at a higher frequency (15.0%) in the under 30 years age group than in the over 30 year age group (2.3%) (p less than 0.001). 3) The unstained inclusion C.T. bodies were confirmed more easily by phase contrast microscopy. 4) Inclusion bodies were specified as either reticulate or elementary bodies by the acridine orange staining method.

Adult↗
PubMed1990

Ultrastructural study of the ciliated cyst in the human uterine tube epithelium.

Ciliated cysts in the human uterine tube epithelium were investigated with the transmission electron microscope. The cysts were about 3-9 microns in diameter and were provided with many ciliary apparatuses and microvilli. Degenerative changes of these cilia, such as electron-dense round or irregular bodies and amorphous substance, were observed in many cysts, but complete disappearance of ciliary structures was not detected in any ciliated cysts. The ciliated cysts were mostly observed in basal cells and were occasionally found in ciliated cells bordering the tubal lumen. In the basal cells, these cysts distended with the increase in degenerated cilia. Distended ciliated-cyst-containing cells became exposed directly to the tubal lumen. U- or reverse omega-shaped deep indentations of the apical surface of ciliated cells confirmed the opening of ciliated cysts into the lumen. It was suggested that the ciliated cysts result from the premature differentiation of basal cells or disturbed migration of centrioles in ciliogenic cells.

Cell Differentiation↗
PubMed1988

HLA DR antigens in adult-onset and juvenile-onset Japanese insulin-dependent diabetic patients.

In order to discover the HLA DR antigens linked to Japanese insulin-dependent diabetes (IDDM), and to relate them to the clinical features, HLA DR antigens were examined in 75 IDDM patients including 56 adult-onset cases. Among the tested HLA DR antigens, 4, w9 and w13 were significantly more frequent in IDDM (55%, 47% and 27% respectively). The relative risk was 1.71 for DR4, 2.81 for DRw9 and 4.74 for DRw13. DR2 was significantly less frequent and the relative risk was 0.14. The distribution of DR antigens did not differ between juvenile-onset and adult-onset IDDM, males and females, or cases with and without thyroid autoantibodies. Homozygotes for DRw9 were, but those for DRw13 and DR4 were not more frequent than expected by a random combination. Heterozygotes for DR4 and w9 were less frequent while other heterozygotes for high-risk antigens were as frequent as expected. 97% of IDDM had either DR4, w9 or w13. In conclusion, HLA DR4, w9 and w13 were significantly increased in patients with both juvenile- and adult-onset IDDM. There was no surplus increase in the frequency of IDDM patients with two high-risk HLA DR antigens, more than expected from random combination of each of these DR antigens. Clinical features did not differ among IDDM patients with each of these three antigens.

Adult↗
PubMedDate not supplied

Intracytoplasmic lumina in human oviduct epithelium.

Intracytoplasmic lumina (ICL) in human oviduct epithelium were investigated with transmission electron microscopy. ICL were found in 43 out of 60 cases examined. They were ultrastructurally characterized by microvilli lining the lumina, periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining-positive finely granular material in the lumina, and secretory vesicles in the cytoplasm surrounding the lumina. Although ICL were observed at various heights within the epithelium, they were mainly seen in basally located cells that did not face the oviduct lumen. Various stages of formation and development of ICL were observed in the basally located epithelial cells with secretory activities. Primary ICL were originated in the cytoplasm where the secretory granules were aggregated with smooth-surfaced tubular vesicles. Electron microscopic observations after PA-TCH-SP staining revealed that ICL were formed by fusion of the secretory granules with the tubular vesicles. ICL were enlarged into round profiles by further fusion of secretory granules and tubular vesicles, and subsequently opened to the oviduct lumen, or fused to each other to develop into large extracellular cysts within the epithelium.

Adult↗