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Biomedical subjects

N Poggi

Publications and source records attributed to N Poggi.

9 recordsLinked to original sources
PubMed1990

[Papillary cystic tumor of the pancreas].

A case of papillary cystic tumour of the pancreas in a young woman is reported. The review of the literature permits to stress the rarity of this tumour with a good prognosis.

Adult↗
PubMed1977

"Immunosubtraction" electrophoresis: a simple method for identifying specific proteins producing the cellulose acetate electrophoretogram.

To identify the proteins that are responsible for the electrophoretic pattern on cellulose acetate, we propose a method based on the precipitation of various individual proteins at the beginning of the electrophoretic run. Any non antibody proteins in the monospecific antisera were removed with a preliminary electrophoretic fractionation of the antiserum. Using this method, we could remove a band or a zone from the pattern, leaving the rest of the pattern unaltered. This permitted us not only to locate a protein in the pattern, but also to better interpret the electrophoretogram. The method requires no special apparatus and it is fast and cheap.

Blood Protein Electrophoresis↗
PubMed1976

The problem of peak identification in crossed immunoelectrophoresis: a modification of the technique of Platt et al.

After a brief review of the known techniques for the identification of crossed immunoelectrophoresis peaks, the authors claim that the technique of Platt et al (1973), Clin. Chim. Acta 46, 419-429) is the most effective. This consists of blocking a protein in the first run, thereby causing the disappearance of the corresponding peak in the second dimension. The advantages of this technique with respect to the others are discussed.

Humans↗
PubMed1975

Two-dimensional immunoelectrophoresis: a new simplified method.

A new method of two-dimensional immunoelectrophoresis is described, in which fixed amounts of both antigen (total proteins) and antibody are employed. When applied to normal human sera, it results in the appearance of 15 peaks accounting for the commonest serum proteins with the only exception of alpha 1-acid glycoprotein. Transferrin, measured by Laurell's or Mancini's technique, is used as an internal standard. The height of the transferrin peak at 300 mg% is determined by a calibration curve. The heights of the various peaks are thus expressed as percentages of that of transferrin at 300 mg%. The method appears to be simple, rapid and antiserum-saving.

Adolescent↗