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Biomedical subjects

N R Wei

Publications and source records attributed to N R Wei.

9 recordsLinked to original sources
PubMed1992

[Immunohistochemistry of the Scarpa's ganglion and the crista ampullaris of patients with Menière disease].

In certain long-lasting cases of Menière's disease with recurrent attacks, despite of saccotomy, the removal of the vestibular ganglion is the only procedure that relieves vertigo. During ganglionectomy of this group of patients all fresh tissues gotten and gathered from operation are frozen immediately and preserved for immunohistochemical assay with direct and indirect incubations. The positive findings such as ASMA+, AEA+ and ANA+ in tissues of patient's own as well as in control tissues further confirmed the results previously reported by us. The immune-complex mediated arteriolar and capillary vasculitis on the basis of an autoimmune disorder as a secondary autoimmune marker might play a dominant role in this patients group. The demyelination of vestibular neurons in the Ganglion Scarpae might be the cause of the Menière symptoms. To confirming the hypothesis, the research plan must be continuously carried out.

Antibodies, Antinuclear↗
PubMed1991

Inner ear fine structures of the hamster in frozen sections used for immunohistochemical assays for inner ear diseases.

Frozen sections of the inner ear of the hamster enable detailed investigations of the fine structures in immunofluorescence assays. At high magnification single mitochondria can be identified by their reactions with an antiserum containing antibodies against mitochondria. In the positive reaction with an antiserum against nuclei, the typical green fluorescence is restricted to the nuclei, which are mostly separated by the surrounding cytoplasm. The method of immunohistochemical assay using frozen sections from the non-decalcified inner ear is very time-consuming and cannot be recommended for the routine diagnosis of inner ear diseases, although it may be useful in research and for studying critical clinical cases.

Animals↗
PubMed1990

Immunohistochemical findings in the vestibular ganglion from a patient with Menière's disease.

Immunofluorescent staining techniques were performed on a series of cryostat sections of the vestibular ganglion taken during ganglionectomy from a patient with Menière's disease. The direct technique using FITC-labelled antiserum proved the presence of immunoglobulins in the patient's blood vessels and endoneural connective tissue. Preincubation with unlabelled antiserum blocked this reaction. An additional positive reaction of ganglion cells was demonstrated by incubating tissue with the patient's serum. These findings prove antibodies in the patient's serum against the autologous ganglion cells. The vestibular ganglion from a patient with dizziness following a skull base fracture served as a control specimen. No autoimmune reaction was found in the ganglion cells when incubation was carried out with the patient's serum. A positive reaction in the blood vessels and connective tissue was less pronounced. The findings of the present study underline the occurrence of immunological reactions in the vestibular ganglion of patients with Menière's disease. A subsequent degeneration of ganglion cells could provoke clinical Menière's attacks.

Antigen-Antibody Complex↗
PubMed1990

Immunohistochemical localization of thymosin beta 9 in bovine tissues.

Using an indirect fluorescent antibody technique with frozen sections, the localization of thymosin beta 9 was investigated for the first time in bovine thymus, spleen, lung, muscle and liver. The antibodies used have been raised against the N-terminal fragment 1-14 of thymosin beta 9 in order to minimize the cross-reactivity with thymosin beta 4 which was found to be also present in bovine tissues. The specific antibodies against thymosin beta 9 raised in our laboratory allowed us to localize this peptide in presence of the highly homologous and always accompanying thymosin beta 4 in different tissues. Although thymosin beta 9 was first isolated from calf thymus, it could be also detected in other bovine organs. The highest density of positive immunoreaction was found to be in spleen sections. In the muscle tissue a pronounced fluorescence intensity was present in the region of the sarcolemma.

Animals↗
PubMed1988

[Specific and nonspecific fluorescence in the use of frozen sections of the cochlea].

Fresh frozen sections of unfixed inner ear tissue of the hamster were dried at room temperature and stored in a refrigerator (+4 degrees) in a wet chamber or at -20 degrees. It was demonstrated that our method for the storage of a tissue bank is suitable for the determination of antibodies in the serum of patients with inner ear diseases. To avoid floating tissue during washing and incubation the fresh frozen sections may also be slightly fixed by covering with gelatine or with a drop of acetone. Both techniques cause non-specific fluorescence. It is most advisable to dry the unfixed sections and to rinse them. Sections of stored renal, brain and inner ear tissue of the hamster also showed both a good preservation of the tissue structures and a perfect result of an antigen-antibody reaction. Relatively strong autofluorescence was found in the stria vascularis, the spiral ligament, the organ of Corti and in the basilar membrane. After incubation with normal serum, non-specific fluorescence was found in addition in the bone and in the tectorial membrane. This non-specific reaction can be avoided by rinsing and counterstaining. Up to now only those antibodies were demonstrated with inner ear tissue that had reacted with other tissues.

Animals↗
PubMed1987

[Optimization of the immunofluorescence test for antibodies in the serum of patients with inner ear diseases].

Antibodies in the serum of patients with inner ear diseases can be demonstrated immunohistochemically with human kidney tissue. In this study a patient's serum with the typical positive reaction against proximal tubuli (antibodies against microsomes) and a serum with antibodies against mitochondria was tested with different dilutions of the fluorescein marked antiserum (FITC conjugate). For both sera a dilution of 1:160 showed positive reaction. The fluorescence of the control incubation was negligible. Employing the given technical details the immunohistochemical assay of antibodies with human kidney tissue has become a simple and reliable test for the most frequently found antibodies in patients with inner ear diseases.

Autoantibodies↗