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Biomedical subjects

N Renault

Publications and source records attributed to N Renault.

4 recordsLinked to original sources

RPBS: a web resource for structural bioinformatics.

RPBS (Ressource Parisienne en Bioinformatique Structurale) is a resource dedicated primarily to structural bioinformatics. It is the result of a joint effort by several teams to set up an interface that offers original and powerful methods in the field. As an illustration, we focus here on three such methods uniquely available at RPBS: AUTOMAT for sequence databank scanning, YAKUSA for structure databank scanning and WLOOP for homology loop modelling. The RPBS server can be accessed at http://bioserv.rpbs.jussieu.fr/ and the specific services at http://bioserv.rpbs.jussieu.fr/SpecificServices.html.

Computational Biology↗

Consumption of soup and nutritional intake in French adults: consequences for nutritional status.

AIM: The impact of soup consumption on nutrient intake and nutritional indicators was assessed in adults who consumed soup compared to those who did not or who were occasional eaters. METHODS: Data were obtained for 2,188 men and 2,849 women living in France and participating in the SU.VI.MAX cohort, who reported twelve 24-h dietary records during a 2-year follow-up period (60,444 records). Subjects were divided into three groups: (1) those who ate soup 0-2 days or less out of 6 days were classified as occasional or non-consumers; (2) those who consumed soup 3-4 days out of 6 were defined as regular consumers; (3) those who consumed soup 5-6 days out of 6 were defined as heavy consumers. RESULTS: Seven per cent of women and 9% of men were heavy consumers of soup. Respectively, 46 and 42% were regular, and 47 and 49% were occasional or non-consumers. Mean energy intake was lower in heavy consumers than in occasional or non-consumers, but the difference was statistically significant only in women. In men and women, heavy consumers of soup had significantly higher intakes of carbohydrates than occasional and non-consumers (245 g J(-1) vs. 227 g J(-1) in men; 186 g J(-1) vs. 176 g J(-1) in women) and lower lipid intakes (97 g J(-1) vs. 102 g J(-1) in men; 80 g J(-1) vs. 75 g J(-1) in women). Soup consumers presented lower energy intake at dinner than light or non-consumers. In soup consumers, breakfast and lunch contained greater amounts of carbohydrates, lipids and proteins, but dinner contained significantly smaller amounts of lipids and proteins. A higher frequency of BMI > 27 kg m(-2) was found in occasional and non-soup consumers; conversely a higher frequency of BMI between 23 and 27 kg m(-2) was found in regular consumers of soup and a higher frequency of BMI < 23 kg m(-2) in heavy consumers. For women, an association was found between occasional or non- consumers and BMI > 25 kg m(-2) and between heavy consumers and BMI < 22 kg m(-2). Likewise an association was found in men only between heavy consumption of soup and lower value of serum cholesterol. CONCLUSION: The present data suggest that the consumption of soups contributes to a balanced diet. Consumption of soup may be beneficial for a healthy nutritional status in the overall population.

Adult↗

Downregulation of the tissue-specific transcription factor Fork head by Broad-Complex mediates a stage-specific hormone response.

Drosophila development is coordinated by pulses of the steroid hormone 20-hydroxyecdysone (20E). During metamorphosis, the 20E-inducible Broad-Complex (BR-C) gene plays a key role in the genetic hierarchies that transduce the hormone signal, being required for the destruction of larval tissues and numerous aspects of adult development. Most of the known BR-C target genes, including the salivary gland secretion protein (Sgs) genes, are terminal differentiation genes that are thought to be directly regulated by BR-C-encoded transcription factors. Here, we show that repression of Sgs expression is indirectly controlled by the BR-C through transcriptional down-regulation of fork head, a tissue-specific gene that plays a central role in salivary gland development and is required for Sgs expression. Our results demonstrate that integration of a tissue-specific regulatory gene into a 20E-controlled genetic hierarchy provides a mechanism for hormonal repression. Furthermore, they suggest that the BR-C is placed at a different position within the 20E-controlled hierarchies than previously assumed, and that at least part of its pleiotropic functions are mediated by tissue-specific regulators.

Animals↗

StGCPRP, a potato gene strongly expressed in stomatal guard cells, defines a novel type of repetitive proline-rich proteins.

Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberosum). It encodes a repetitive proline (Pro)-rich protein of 54 kD (491 amino acids) and was named StGCPRP (S. tuberosum guard cell Pro-rich protein). StGCPRP has a bipartite structure. The C-terminal part of StGCPRP contains a high percentage (46%) of Pro residues organized in distinct repetitive sequence motifs, whereas its extended N terminus is essentially free of Pros. StGCPRP represents the first member of a novel class of hybrid Pro-rich proteins that we designated NHyPRPs. In young but not in mature leaves, StGCPRP transcripts were also present at high levels in mesophyll cells (in addition to guard cells), indicating developmental regulation of StGCPRP gene expression. In addition, StGCPRP expression is regulated by environmental factors, as shown by a decrease in StGCPRP transcript levels under drought stress. Two proteins similar to StGCPRP were found to be encoded by the Arabidopsis genome, indicating that NHyPRPs are more widely distributed in higher plants.

Amino Acid Sequence↗