[Effect of glucose admixtures in fructose specimen on the growth (yield of biomass) of Streptomyces roseoflavus var. roseofungini].
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Biomedical subjects
Publications and source records attributed to N S Agre.
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Endospores of Streptomyces streptomycini B-16 are considerably more thermostable (by 40degreesC) than it was generally accepted. The bulk of populations of the spores (up to 55%) can be subjected to 100degreesC during 10 min but does not germinate under usual conditions as a result of heat damage which may be repaired at a relative humidity of 100% or in water suspensions at temperatures optimal for growth of the actinomycete. The rate of reparation increase upon washing of the heated spores with stirring, centrifuging and especially after treating the spores with ultrasound. The treatment is supposed to eliminate from the spores growth inhibitors which were formed as a result of heat damage. Characteristics of the process are discussed, which make it different from other reparation systems described elsewhere, in particular its endogenous character.
IR spectra of the whole cells of oligosporous actinomycetes were studied. These spectra can be used as an additional criterion during identification of actinomycetes at the generic level, and confirm the heterogeneity of the Micropolyspora genus so that some of its species may be classed as an individual genus (Kalakoutskii et al., 1968; Agre, Dorokhova, 1972).
A synthetic medium was used to obtain the dormant spores of Thermoactinomyces vulgaris. The fraction of the dormant spores depended on the amino acid composition of the growth medium. The rate of growth and development of the organism on the synthetic medium is lower as compared to the routinely employed complex medium.
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A new species of the Actinomadura genus, A. fastidiosa sp. nov., is described. The ultrastructure of the vegetative mycelium and spores of this organism was studied. The vegetative cells have a multilayered cell wall, often consisting of five layers with different thickness and electron density. The spores are similar to the vegetative cells by their inner structure but have a thicker wall.