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Biomedical subjects

N S Sergeeva

Publications and source records attributed to N S Sergeeva.

At least 19 recordsLinked to original sources
PubMed2006

Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro.

The dynamics of growth and proliferative activity of the population of multipotent mesenchymal stromal cells from rat bone marrow was studied during 7 passages. The efficiency of colony formation, the morphology of multipotent mesenchymal stromal cells, and the possibility of spontaneous and induced differentiation were studied. The rat bone marrow fibroblast-like multipotent mesenchymal stromal cells are capable of clonal growth; their proliferative activity and the yield remained high until passage 4, but then decreased. Induction of osteo- or adipogenic differentiation of bone marrow multipotent mesenchymal stromal cells increased the percentage of morphologically modified cells carrying specific markers.

Animals↗
PubMed2006

[Application of cell technologies to tissue defect closure in oncology].

In vivo and in vitro experiments on the application of cell technologies to tissue defect closure were conducted; autologic mesenchymal stem cells on 3-demensional matrices were used. The authors analyze the results of the application of bioengineering tissue equivalents for the closure of soft tissue and upper airway defects after extensive resections performed in 52 oncological patients. Tissue equivalents with stem cells provide engraftment and long-term graft functioning; they also modify wound surface, thus stimulating wound epithelization. In this study the application of tissue equivalents led to wound healing and functional recovery in 87% of patients.

Follow-Up Studies↗
PubMed2003

[Tumor-associated antigen CA-125 in patients with ovarian cancer receiving various combinations of therapy].

A dynamic immuno-enzymatic assay of CA 125 was carried out in 43 patients (230 tests) with ovarian tumors receiving chemotherapy alone (group 1), surgery + adjuvant chemotherapy (group 2), or neoadjuvant chemotherapy + surgery + adjuvant chemotherapy (group 3). Mean concentration of CA 125 before treatment was 1,635.0 (362.8 Unit/ml. Direct correlation between number of cases with normal level and that of chemotherapy courses received was established in group 1. After 4-7 courses, the antigen level came back to normal in 80.0%. However, chemotherapy alone failed to bring the marker concentration down below 5.0 Unit/ml, which is believed to promise favorable prognosis. In group 2, CA 125 came back to normal after adjuvant chemotherapy in all patients. The best results in that group were obtained following 3 courses: 5.0 Unit/ml--41.7%, and < 10.0 Unit/ml--75%. Additional 1-3 courses failed to improve the results. Normal concentration was reported after first-line adjuvant chemotherapy in 63.6-75.0% in group 3. The best results after a second course of adjuvant chemotherapy were: > 5.0 Unit/ml--12.5%, and > 10.0 Unit/ml--56.3%.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed2001

Effects of various modes of sonication with low frequency ultrasound on in vitro survival of human tumor cells.

The effect of low frequency ultrasound in the cavitation (5-15 sec exposure) and subcavitation (5-210 sec exposure) modes on in vitro survival of cultured human tumor cells was studied. Analysis of the dose-effect curves and mathematical estimation of the effects low-frequency ultrasonication in the subcavitation mode on cell membranes helped to choose time intervals of tumor cell sonication ensuring potentiation of the cytostatic effects.

Cell Survival↗
PubMed1997

Response to radiotherapy of human uterine cervix carcinoma is not correlated with rearrangements of the Ha-ras-1 and/or c-myc genes.

An association between the presence of the activated form of Ha-ras-1 and c-myc genes and increased cellular radioresistance has been shown in several cell lines. The aim of this study was to determine whether such an association could be observed in clinical tumour biopsies. We examined 70 tumour specimens and 51 samples of peripheral blood obtained from untreated patients with carcinoma of the uterine cervix mainly stage II and III. In addition to initial clinical tumour response to radiotherapy, radiosensitivity was also analysed by the subrenal capsule assay (SRCA). Mutations in exons 1 and 2 of the Ha-ras-1 gene were examined using PCR single-strand conformation polymorphism (PCR-SSCP) and direct sequencing; and restriction fragment length polymorphism of the Ha-ras-1 gene was analysed using Southern hybridisation. Eight (11%) out of 70 tumours contained mutations in exons 1 and 2 of the Ha-ras-1 gene. Eleven (22%) out of the 51 tumours displayed rearrangements of the Ha-ras-1 gene (six tumours (12%) showed alterations of allele length, one amplification and four (8%) loss of one Ha-ras-1 allele). In addition, 12 (17%) out of 70 patients demonstrated the presence of rare alleles. Only one of the 70 tumours contained an amplified c-myc gene. There was no significant correlation between either rearrangements of the structure of the Ha-ras-1 and/or c-myc genes or presence of rare alleles in tumours and tumour response to radiotherapy.

Alleles↗
PubMed1991

[The proliferative activity of stomach cancer and evaluation of its individual radiosensitivity].

An indirect immunofluorescent method with polyclonal antibodies to thymidine was used to assess the proliferative activity (PA--percent of cells in the S-phase of the cell cycle) of 79 stomach tumors from primary cancer patients. Stomach cancer PA was shown to vary from 0.1 to 69.7%. PA did not depend either on a tumor size or a degree of the involvement of regional lymph nodes. The mean PA of well-differentiated adenocarcinoma was slightly lower (14.9 +/- 4.7%) than that of low differentiated ones. Of 79 patients a tumor process was interpreted as a resectable one in 62. They were given preoperative irradiation at a total focal dose of 36 Gy followed by operation. In addition to initial investigation PA in tumor biopsy specimens was assessed after delivering a dose of 12 Gy. PA changes at the beginning of a course of irradiation were compared with a degree of a radiation injury of tumor tissue after a course of irradiation was discontinued in 32 (explorative laparotomy was performed in 30). It was shown that tumor radioresistance could be predicted in unchanged PA indices of their increase at the beginning of a course of irradiation with the probability of 95%.

Adenocarcinoma↗
PubMed1991

[A comparative evaluation of 2 methods for cloning human lung tumors].

Clonogenic capacity of tumor cells from 59 human lung cancer specimens was investigated in Petri dishes (24 specimens) and in capillaries (40 specimens), and parallel cloning was done in 5 cases. The results were as follows: (a) bacterial contamination in capillaries was noted less frequently than in Petri dishes (12.5 vs. 25%); (b) cells did not form colonies in capillaries in 42.5% and in Petri dishes in 25%; (c) colony-forming effectiveness in capillaries was 3.46-fold higher than that in Petri dishes; (d) the amount of tumor cells for analysis in capillaries is 10-fold less than that for analysis in Petri dishes. However, the absolute number of colonies, indispensable for assessment of tumor sensitivity to antitumor action (not less than 15 colonies per capillary or Petri dish) was obtained in 67% in Petri dishes and in 33% only in capillaries.

Adenocarcinoma↗