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Biomedical subjects

N Sari

Publications and source records attributed to N Sari.

At least 19 recordsLinked to original sources

Consumer out-of-pocket spending for pharmaceuticals in Kazakhstan: implications for sectoral reform.

What do consumers pay for pharmaceuticals in a transition economy, and who is hit hardest? Kazakhstan is in the midst of emerging from a Soviet Union state to a market economy. It has seen a significant dip in Gross Domestic Product and available revenues for health as a result. New sources of revenues, such as out-of-pocket payments, both formal and informal, have become widespread. In this paper we use the results of a 1996 Living Standards survey jointly sponsored by the World Bank and the Kazakhstan Government to examine patterns of prescribed pharmaceutical spending. We use a two-part regression model that is utilized to adjust for the skewness of non-spenders and heavy utilizers. Results suggest that upper-income groups spend more in absolute terms, but low-income groups pay a higher share of their income for pharmaceuticals. Pharmaceutical expenditure is positively related to poor health status, chronic illness and rural area residence. Our estimates suggest that on average people in rural areas spend 16% more than people in urban areas. The analysis shows that certain types of illnesses impose significant out-of-pocket burden for consumers - gynaecologic as well as intestinal and cardiac. The findings can be used for developing and designing a new 10-year World Bank-financed programme for restructuring the health sector. They also suggest the need for prioritizing rural care, as well as covering pharmaceuticals for specific types of care interventions and certain demographic groups.

Community Participation↗

Turkey and its international relations in the history of medicine.

My discussions with ISHM members have disclosed considerable interest in the history of the relations that Turkey and its medicine have had with other countries. Dr Lellouch, the secretary of ISHM, originally suggested that I address the subject of Turkish-French relations by means of an essay in Vesalius. This led me to consider a wider ranging paper on Ottoman-European relations. For completeness, I have briefly covered the Turkish peoples' relations with the Eastern, as well as the Western World. The overall aim of this article is to act as a stimulus for further discussion on the international relations in health sciences between Turks and other peoples.

History, Ancient↗

Structure and dynamics of an acid-denatured protein G mutant.

NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 degrees C, F30H-G(B1) is greater than 95% folded at pH 7.0 and is greater than 95% unfolded at pH 4.0. This range of stability is useful because the denatured state can be examined under relatively mild conditions which are optimal for folding G(B1). We have assigned almost all backbone (15)N, H(N), and H(alpha) resonances in the acid-denatured state. Chemical shift, coupling constant, and NOE data indicate that the denatured state has considerably more residual structure when studied under these mild conditions than in the presence of chemical denaturants. The acid-denatured state populates nativelike conformations with both alpha-helical and beta-hairpin characteristics. To our knowledge, this is the first example of a denatured state with NOE and coupling constant evidence for beta-hairpin character. A number of non-native turn structures are also detected, particularly in the region corresponding to the beta1-beta2 hairpin of the folded state. Steady-state ¿(1)H-(15)N¿ NOE results demonstrate restricted backbone flexibility in more structured regions of the denatured protein. Overall, our studies suggest that regions of the helix, the beta3-beta4 hairpin, and the beta1-beta2 turn may serve as potential initiation sites for folding of G(B). Furthermore, residual structure in acid-denatured F30H-G(B1) is more extensive than in peptide fragments corresponding to the beta1-beta2, alpha-helix, and beta3-beta4 regions, suggesting additional medium-to-long-range interactions in the full-length polypeptide chain.

Amino Acid Sequence↗

NMR spectroscopy, molecular dynamics, and conformation of a synthetic octasaccharide fragment of the O-specific polysaccharide of Shigella dysenteriae type 1.

A synthetic octasaccharide fragment (2) of the O-specific polysaccharide (1) of Shigella dysenteriae type 1 has been studied as its methyl glycoside by one- and two-dimensional homo- and heteronuclear NMR spectroscopy. Complete 1H and 13C NMR assignments have been generated, and the 13C spin-lattice relaxation times have been measured for the octasaccharide 2. A congener (6) of this octasaccharide containing one D-galactose residue with a specific 13C label at C-1 has been synthesized and used to measure interglycosidic 13C-1H coupling by the 2D J-resolved 1H NMR method. From the NMR data, three types of conformational restraints were developed: (a) 29 inter-residue, distance restraints; (b) 48 intra-residue, ring atom dihedral angle restraints, and (c) one heteronuclear, inter-residue dihedral angle restraint. The use of these restraints in a restrained molecular dynamics computation with simulated annealing yielded a conformation resembling a short, irregular spiral, with methyl substituents on the exterior.

Carbohydrate Conformation↗

Comparison of backbone dynamics of oxidized and reduced putidaredoxin by 15N NMR relaxation measurements.

The backbone dynamics of uniformly 15N-labeled reduced and oxidized putidaredoxin (Pdx) have been studied by 2D 15N NMR relaxation measurements. 15N T1 and T2 values and 1H-15N NOEs have been measured for the diamagnetic region of the protein. These data were analyzed by using a model-free dynamics formalism to determine the generalized order parameters (S2), the effective correlation time for internal motions (tau e), and the 15N exchange broadening contributions (Rex) for each residue, as well as the overall correlation time (tau(m)). Order parameters for the reduced Pdx are generally higher than for the oxidized Pdx, and there is increased mobility on the microsecond to millisecond time scale for the oxidized Pdx, in comparison with the reduced Pdx. These results clearly indicate that the oxidized protein exhibits higher mobility than the reduced one, which is in agreement with the recently published redox-dependent dynamics studied by amide proton exchange. In addition, we observed very high T1/T2 ratios for residues 33 and 34, giving rise to a large Rex contribution. Residue 34 is believed to be involved in the binding of Pdx to cytochrome P450cam (CYP101). The differences in the backbone dynamics are discussed in relation to the oxidation states of Pdx, and their impact on electron transfer. The entropy change occurring on oxidation of reduced Pdx has been calculated from the order parameters of the two forms.

Amides↗

Exploration of the structural environment of the iron-sulfur cluster in putidaredoxin by nitrogen-15 NMR spectroscopy of selectively labeled cysteine residues.

Putidaredoxin is a di-iron protein whose paramagnetic region is not well characterized by 1H detected NMR. We have studied the structure of this region in greater detail by directly observed 15N NMR of oxidized and reduced putidaredoxin preparations in which the six cysteine residues are selectively labeled with 15N. A new method for preparation of a stable form of reduced putidaredoxin has been developed for use in NMR. The 15N NMR spectra of the oxidized and reduced forms are characteristically different, and we have measured and compared 15N chemical shifts, spin-lattice relaxation times (T1), and chemical shift/temperature dependences for both forms. Evidence for localized valencies of the iron atoms in the reduced form is presented. From the 15N T1 values of the oxidized form, reduced distances of the cysteine backbone 15N nuclei from the center of the Fe2S2 cluster have been calculated. These distances are consistent with those calculated from X-ray crystal structure data for five ferredoxins, and confirm the structural similarity of the Fe2S2 clusters in putidaredoxin and in these ferredoxins in the oxidized state.

Amino Acid Sequence↗

Measurement of interglycosidic 3JCH coupling constants of selectively 13C labeled oligosaccharides by 2D J-resolved 1H NMR spectroscopy.

Tri-, tetra-, and penta-saccharide fragments of the O-specific polysaccharide of Shigella dysenteriae type 1 have been prepared in which a D-galactose residue of each oligosaccharide methyl glycoside derivative contains a 13C label at C-1. The interglycosidic coupling constants (3JCH) of these 13C nuclei with the H-3 nuclei of the adjacent 2-acetamido-2-deoxy-D-glucose residues have been measured by two-dimensional, J-resolved 1H NMR spectroscopy. The magnitudes of these coupling constants indicate that the trisaccharide is conformationally different to the higher oligosaccharide homologs, in agreement with previous studies of 13C chemical shifts and 1JCH values.

Carbohydrate Sequence↗

The drug "Hindiba".

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History, Medieval↗

[Not Available].

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History, Modern 1601-↗

Circumcision ceremonies at the Ottoman palace.

Circumcision is widely practiced in all Islamic countries. Festivities pertaining to circumcision vary according to the regions and civilizations. In this report, circumcision festivities at the Ottoman Palace and the socioeconomic importance of the tradition are presented. The Ottoman circumcision technique is discussed, as are the miniature paintings, in manuscript, written on the occasion of the circumcision of the sons of the Sultans. Because these festivities involved the participation of all classes of the society and all professions they contributed to social and technical progress and led to developments in art, music, sports, and ideas.

Circumcision, Male↗

[Qualifications and morality requisite for the personnel to be employed in the Ottoman Hospitals (Dar-al-Shifas)].

Ottomans legislated various codes and founded several institutions to provide care and protection for the sick, disabled, orphans, widows, invalids and the aged. Sultans passed acts assigning a proportion of the tax income to the needs of the diseased and disabled people in hospitals (dar-al-shifas) and nursing; and created foundations of health with trusts of deeds (waqfiyyes). These deeds comprise interesting information on hospital management as well as the duties, responsibilities, qualities, and proficiency standards requisite for physicians and other employees of these institutions. This article deals with the trusts of deeds of the dar-al-shifas founded by the Sultans, Bayazid I. in 1400, Mehmed II. the Conqueror in 1470, Bayazid II. in 1488, Süleyman the Magnificent in 1556, Sultan Ahmet I in 1616; and the Sultans' wifes', Hafsa's in 1539, Haseki Sultan Hurrem's in 1550, and Nurbanu's in 1582, in view of the characteristics stated above. A Hospital which presumably had a capacity of 20 to 50 patients had a staff consisting of approximately 20 to 25 employees, covering primarily, physicians (tabib), ophtalmologists (kahhal), surgeons (djarrah) and assistant health personnel such as pharmacists (ashshab), cook-dieticians (tabbah), a drug-smasher (adviyekub), a cellar-keeper (kilardje), nurses (kayyum), an attendant of kitchen ware (qassa-kash), sweepers (farrash), a W.C. cleaner (ab-reze) and a doorkeeper (bevvab) etc. ...

Health Personnel↗

[A short look on the protection of the disabled in Turkish history until the Republic of Turkey I].

This study deals with the care taken through Turkish history to protect and shelter the disabled orphans, widows, crippled and the aged, etc. In the Seljukian and Ottoman periods there were different foundations which shouldered the duty and functioned as almhouses. Some of these foundations had houses for sheltering the disabled and the homeless, according to those quoted in the trust of deeds. These institiutions were "Dār-al rahā" (almhouse); Tāb-khāne" and "Imāret" (almshouse and soup kitchen for the poor), "Caravanserai" (inns), "Musāfir-khāne" (public guest house for travelers); "Dār-al ziyāfe" (temporary boarding house and kitchen); "Dār-al shifā" (hospital) and "Tekye" or "Zāviye" (dervish lodges), etc. ...

Persons with Disabilities↗

Serafeddin Sabuncuoğlu, the author of the earliest pediatric surgical atlas: Cerrahiye-i Ilhaniye.

The author of one of the earliest surgical books was Serafeddin Sabuncuoğlu, who was born in one of the ancient cities of Central Anatolia. In 1465, he wrote a surgical book in Turkish. The aim of this study was to investigate the details of this book and compare it with the old classics. It was observed that the book of Sabuncuoğlu did not contain only pictures or miniatures of pediatric surgical procedures, but there were many important and major new contributions to the surgical literature originally described by Sabuncuoğlu himself. He based his contributions and techniques on formerly designed and described procedures, moreover, developing and nourishing pediatric surgical culture of that era. Thus a combination of Greek, Roman, Arabic, and Turkish pediatric surgery combined extraordinarily and influenced the development of European pediatric surgery.

General Surgery↗

[Not Available].

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Education, Medical↗

[Not Available].

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Historiography↗