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N Skinner

Publications and source records attributed to N Skinner.

5 recordsLinked to original sources
PubMed1991

A low molecular weight endometrial secretory protein which is increased by ovine trophoblast protein-1 is a beta 2-microglobulin-like protein.

Ovine trophoblast protein-1 (oTP-1), stimulates the secretion of several proteins in explant culture of day-12 cyclic ovine endometrium. We partially purified and identified one of these proteins, an 11,000 Mr, pI approx. 6 protein by N-terminal amino acid sequencing and immunoprecipitation using antibody to human beta 2-microglobulin. The protein was purified from cultures of endometrium collected from day-16 pregnant ewes. The N-terminal amino acid sequence was 40-55% homologous to beta 2-microglobulin from a variety of species. Antibody to human beta 2-microglobulin immunoprecipitated the protein and another protein of similar molecular weight but more acidic pI. Using immunoprecipitation of radiolabelled proteins from culture, we demonstrated that oTP-1 increased production of this protein by 40% (P less than 0.05). We conclude that oTP-1 increases the secretion of a beta 2-microglobulin-like protein from day-12 non-pregnant endometrium in culture.

Amino Acid Sequence↗
PubMed1967

Methylphenidate and stimulus control of avoidance behavior.

The introduction of a warning signal that preceded a scheduled shock modified the temporal distribution of free-operant avoidance responses. With response-shock and shock-shock intervals held constant, response rates increased only slightly when the response-signal interval was reduced. The result is consistent with Sidman's (1955) findings under different conditions, but at variance with Ulrich, Holz, and Azrin's (1964) findings under similar conditions. Methylphenidate in graded doses increased response rates, modifying frequency distributions of interresponse times. Drug treatment may have disrupted a "temporal discrimination" formed within the signal-shock interval. More simply, methylphenidate influenced response rates by increasing short response latencies after signal onset; this effect was more prominent than the drug's tendency to increase the frequency of pre-signal responses. When signal-onset preceded shock by 2 sec, individual differences in performance were marked; methylphenidate suppressed responding in one rat as a function of increasing dose levels to a greater degree than in a second animal, but both subjects received more shocks than under control conditions.

Animals↗
PubMedDate not supplied

Temporal characteristics of evaluation anxiety.

The temporal characteristics of evaluation anxiety are not well-defined by previous research. We examined the effects of length of the pre-evaluation interval (3, 6, or 12 minutes) and stage of the pre-evaluation interval at which evaluation anxiety was measured (start, middle, or end) on evaluation anxiety while participants performed an activity that was the focus of the impending evaluation. Participants wrote their opinion on a controversial social issue while anticipating a subject matter expert's judgment of their social maturity, and evaluation anxiety was measured by a battery of state anxiety measures. Higher levels of evaluation anxiety were detected on the Worry-Emotionality Questionnaire (WEQ) Worry subscale at the end of the pre-evaluation interval than at earlier stages, regardless of interval length, although individual difference variables exerted an important influence. Individuals with high trait self-presentation concerns experienced particularly high state anxiety (State-Trait Anxiety Inventory) at the start of the longest pre-evaluation interval. Low self-efficacy individuals showed a U-shaped pattern across pre-evaluation stages on both the WEQ Worry and Emotionality subscales, while high self-efficacy participants showed either no change (worry) or an inverted-U pattern (emotionality). Implications for the experimental measurement of evaluation anxiety were discussed.

Adolescent↗