Biomedical subjects

N Sukcharoen

Publications and source records attributed to N Sukcharoen.

34 records · Page 2Linked to original sources

PubMed1995

Evaluation of sperm nuclear DNA normality by acridine orange staining technique.

To determine the relationship between sperm deoxyribonucleic acid (DNA) normality and fertilizing potential, 93 semen samples from 48 fertile donors and 45 male partners of infertile couples whose major abnormalities in the female partner had been ruled out were studied. Semen samples were assessed for conventional parameters (volume, percentage of normal morphology, percentage of progressive motility, sperm concentration, and round cell concentration) according to World Health Organization (WHO) guideline and acridine orange staining. The mean sperm concentration, percentages of progressive motility, and percentage of green fluorescing sperm in semen were significantly higher in samples from fertile donors (p < 0.005). When the parameters of semen quality were considered normal according to the standard WHO criteria, the mean percentages of green-fluorescing sperm were significantly higher in fertile donors than infertile patients (65.6% vs 53.3%; p < 0.05). Therefore, the acridine orange staining technique for evaluation of the sperm DNA normality appears to give more information in infertile patients with normal semen analysis. It may be a useful addition to the conventional semen analysis.

Acridine Orange↗

PubMed1995

Evaluation of the Hamilton-Thorn Motility Analyzer for routine semen analysis.

In routine semen analysis of 102 patients the values of sperm concentration and the percentage of sperm motility were measured with the Hamilton-Thorn Motility Analyzer (HTMA) and compared to the data obtained by conventional semen analysis according to the guidelines of the World Health Organization (WHO). Results were classified according to the conventional semen analysis before statistical analyses. Overall, sperm concentration by both methods showed a correlation of 0.95 (p < 0.0001) but the HTMA gave higher values for sperm concentration (mean difference 10.8 +/- 18.9 x 10(6)/ml, mean +/- S.D.). Only a sperm concentration of between 40 and 80 x 10(6)/ml can be measured accurately with the HTMA. The percentage of sperm motility by both methods showed a correlation of 0.759 (p < 0.0001) but were estimated slightly lower by the HTMA than by conventional analysis (mean difference 1.7 +/- 15.8%). In conclusion, the HTMA system can not replace conventional semen analysis in routine semen analysis.

Evaluation Studies as Topic↗

PubMed1994

A comparison of Makler counting chamber and improved Neubauer hemocytometer in sperm concentration measurement.

Evaluation of male fertility is based predominantly on results from semen analysis and determination of the sperm concentration is one of the main parameters of the analysis. To assess the accuracy of sperm concentration measurements by Makler counting chamber, manual sperm counting of 55 semen samples were made using a Makler counting chamber, and compared with concentration values measured using an improved Neubauer hemocytometer according to the World Health Organization guideline (standard procedure). Results were classified according to the standard procedure before statistical analyses. Both values correlated well. Sperm concentration obtained with Makler counting chamber was not statistically different from those determined by improved Neubauer hemocytometer in semen samples with concentrations over 40 x 10(6)/ml. But using Makler counting chambers caused a shift concentrations, which were overestimated significantly (p less than 0.0001) in semen samples with concentrations less than 40 x 10(6)/ml. Overall, Makler chamber counts were 11.2 per cent higher. Although less complicated than the improved Neubauer hemocytometer method, measurement of sperm concentration by Makler counting chamber is an inaccurate method, especially in semen samples with concentrations less than 40 x 10(6).

Bias↗

PubMed1994

Sonographic prenatal diagnosis of heterozygous achondroplasia: a case report.

A case of heterozygous achondroplasia diagnosed prenatally by sonographic examination at third trimester, demonstrating a large head, frontal bossing, low nasal bridge, shortening of the long bones (rhizomelic form), was presented and confirmed by neonatal physical examination and radiograph. Sonography has become the most sensitive and accurate method for detection of this abnormality. It displays the fetal biometry without distortion and permits accurate prenatal diagnosis. However, the prenatal diagnosis of this abnormality is very difficult until the third trimester of pregnancy.

Achondroplasia↗

PubMed1994

The effect of discontinuous Percoll gradients preparation on sperm movement characteristics.

To determine the improvement of sperm movement characteristics after discontinuous Percoll gradients preparation, sperm movement characteristics from 60 normal semen samples were assessed before and after discontinuous Percoll gradients preparation using Hamilton-Thorn Motility Analyzer (HTMA). There was a significant correlation between manual and HTMA results for the percentage of motile sperm (r = 0.8670, p < 0.001). The percentage rapid, percentage motile, average path velocity (VAP), progressive velocity (VSL), curvilinear velocity (VCL), beat cross frequency (BCF), amplitude of head displacement (ALH), percentage of progressive motility, and hyperactivation were significantly increased after preparation. Therefore, this sperm preparation technique is a convenient and efficient method to prepare progressive motile sperm for assisted conceptive technology.

Cell Survival↗

PubMed1994

Efficiency of discontinuous Percoll gradients preparation for selection of motile sperm from poor quality semens.

Efficiency of discontinuous Percoll gradients preparation for selection of motile sperm from poor quality semens was studied in 106 semen samples, of which 52 were normozoospermia, 9 were oligozoospermia, 29 were asthenozoospermia, and 16 were oligoasthenozoospermia. Sperms from each semen sample were recovered by the discontinuous two-layer (50%/100%) Percoll centrifugation technique. Overall motile sperm recovery rate was about 27 per cent. The results obtained in this study indicated that sperm selection by this method from oligozoospermic and oligoasthenozoospermic semen samples was not as good as normozoospermic semen samples because per cent progressive motility showed no statistically significant improvement after sperm selection in oligozoospermic and oligoasthenozoospermic semen samples. Moreover, total sperm recovery rate and motile sperm recovery rate of oligozoospermic semen samples were less than normozoospermic samples significantly (p < 0.01 and p < 0.005, respectively).

Centrifugation, Density Gradient↗

PubMed1994

Effects of pentoxifylline on sperm motility characteristics and motility longevity of postthaw cryopreserved semen using computer-assisted semen analysis (CASA).

Computer-assisted sperm movement analysis was used to study the effect of pentoxifylline on human sperm motility characteristics and motility longevity of postthaw cryopreserved semen. This study focused on the following issues: the changes in individual movement characteristics in response to pentoxifylline, the persistence of the response during drug treatment. Computerized analysis was started at 30 min, 3 hrs and 24 hrs, after addition of pentoxifylline. Data obtained showed that pentoxifylline significantly increased percentage of sperm motility, average path velocity (VAP), curvilinear velocity (VCL), straight line velocity (VSL), amplitude of lateral head displacement (ALH) at two of the three time periods (p < 0.05). But, it did not significantly increase linearity (LIN), straightness (STR), and beat cross frequency (BCF) at any time. After 24 hrs, all motility variables were significantly decreased in both groups. However, the 24-hr motility longevity in the treatment group was greater than that of the control group. The present analysis shows interest in the use of pentoxifylline as a sperm movement enhancer for postthaw cryopreserved semen. It shows a beneficial effect in the majority of sperm movement parameters and motility longevity which may increase pregnancy rates after insemination. However, whether this change leads to an increase in fertilizing ability requires further study.

Cryopreservation↗

PubMed1993

Sonographic prenatal diagnosis of congenital heart defects in thoraco-omphalopagus.

Thoraco-omphalopagus is the most common variety of conjoined twins. About 90% of the conjoined thoraco-omphalopagus twins have a common pericardium, and 75% have conjoined hearts. Options for the treatment of thoraco-omphalopagus are largely dependent on the anatomy of the cardiovascular system. The extent of the conjunction of the heart and the severity of the associated cardiac defects primarily determine the feasibility of successful separation of the infants. Two-dimensional echocardiography and color Doppler examination has become the method used to investigate cardiovascular system of conjoined twins with a high degree of confidence. A case of thoraco-omphalopagus conjoined twins prenatally determined of the extent of cardiac conjunction and diagnosis of associated defects by these methods is presented.

Adult↗

PubMed1993

Effects of digital cervical examinations on duration of latency period, maternal and neonatal outcome in preterm premature rupture of membranes.

The medical records of 115 singleton pregnancies by preterm PROM from Chulalongkorn Hospital that met the criteria for expectant treatment from January 1987 to December 1991 were reviewed. All cases were divided into 2 groups. Fifty cases had a digital cervical examination to assess cervical status and sixty-five cases had a sterile speculum examination only. Latency period, maternal and neonatal outcome of both groups were compared. There was no statistically significant difference between the latency period of both groups (34.19 +/- 78.35 versus 54.6 +/- 127.01 hours). This was consistent for all gestational age groups. There was statistically significant increased incidence of chorioamnionitis in patients receiving digital cervical examinations. A nonsignificant trend toward an increased incidence of endometritis was also noted in patients receiving digital cervical examinations. There was no statistically significant differences in the average time of delivery, mode of delivery and postpartum hospitalization between both groups. No maternal mortality and stillbirth was noted in this study. There were no statistically significant differences in Apgar score at 1 minute and 5 minute, NICU admittance, neonatal hospitalization and percentage of neonatal deaths, livebirths, and neonatal morbidity between both groups. The results of this study suggest that digital cervical examinations in patients with preterm PROM should be avoided until the clinician is convinced that the patient is clearly in labor because digital cervical examinations increase the incidence of chorioamnionitis and endometritis but have no effect on latency period.

Adult↗

PubMed1993

Prenatal sonographic diagnosis of meconium peritonitis: a case report.

A case of meconium peritonitis that was diagnosed ultrasonographically in the third trimester is presented. Fetal ascites, intra-abdominal calcification, left communicating hydrocele, and polyhydramnios were detected on antenatal ultrasonography. Specks of calcification were also demonstrated on abdominal radiography postnatally. Laparotomy confirmed the diagnosis of perforated terminal ileum with meconium peritonitis. The obstetric and neonatal implications of meconium peritonitis are discussed with literature review.

Adult↗

PubMed1992

Biparietal diameter/femur length ratio and actual femur length/expected femur length ratio: a sonographic screening method for Down's syndrome.

Several ultrasonographic signs have been described in second-trimester Down's syndrome fetuses. Previously published reports have shown that fetuses affected with Down's syndrome have normal biparietal diameter (BPD), high BPD-to-femur length (FL) ratio, and low actual FL-to-expected FL ratio. A retrospective comparison of the BPD-to-FL ratio and actual FL-to-expected FL ratio was made between 3 fetuses with Down's syndrome diagnosed prenatally by second-trimester amniocentesis and 189 normal fetuses with gestational age varying from 13-25 weeks who were seen in the Ultrasound Unit of Department of Obstetrics & Gynaecology, Chulalongkorn Hospital between January 1, 1989 and May 31, 1990. The sensitivity of BPD-to-FL ratio and actual FL-to-expected FL ratio as a screening technique for Down's syndrome in this study was 66.7 and 100 per cent, with a specificity of 93.4 and 89.2 per cent respectively. These sonographic parameters appear to be a useful screening method for Down's syndrome.

Down Syndrome↗

PubMed1992

Transvaginal sonographic features in ectopic pregnancy.

A retrospective review of the transvaginal sonograms of 37 women with confirmed ectopic pregnancy was performed to characterize the findings from transvaginal sonography and correlate operative findings with sonographic features. Thirty-six of the 37 ectopic pregnancies were tubal pregnancies. Intrauterine findings showed an absence of gestational sac in all cases and a pseudosac in 1 case (7.14%). An ectopic gestational sac with a live embryo was documented in 2 cases (5.41%) and with embryo or yolk sac in 6 cases (16.22%) and nonspecific adnexal mass in 30 cases (81.08%). Fluid in the cul-de-sac was found in 22 cases (59.46%). Transvaginal sonography provides better access to the pelvic organ and allows a clear view of the uterus and adnexa, with no need to fill the bladder. Excellent visualization of the endometrial cavity permits the detection of even a very early intrauterine pregnancy and clearly shows the gestational sac where it is outside the uterine cavity. Blood and other fluids in the cul-de-sac can also be seen easily. This technic has reduced patients' risk by minimizing delay in diagnosis and treatment.

Female↗

PubMed1992

Pelvic actinomycosis in pregnancy: a case report and review of the literature.

The first reported case in Thailand of pelvic actinomycosis during pregnancy is presented. It occurred in a 38-year-old Thai female with a history of IUD wearing for 2 years until the symptoms of intermittent lower abdominal pain. She had a history of postcoital bleeding at 16 weeks' pregnancy and bleeding per vagina during labor at 40 weeks' pregnancy. Speculum examination showed an irregular, friable, easily bleeding mass (3-4 cm in diameter). The specimens of the mass at the posterior fornix were obtained for culture and pathological examination. Right tubo-ovarian complex measured 10 x 4 x 4 centimeters in diameter, extending through the cul-de-sac to the vaginal canal at the posterior fornix, was identified during exploratory laparotomy. Low transverse cesarean section was performed. The pathological diagnosis was actinomycosis of right fallopian tube and ovary. The patient was treated with tetracycline.

Actinomycosis↗

PubMed1991

Maternal perception of sound-provoked fetal movement as a test of antepartum fetal wellbeing.

Maternal perception of sound-provoked fetal movement test was studied on 506 occasions in 443 women with obstetric or medical antenatal risk factors after 26 weeks gestation. The response was compared with a nonstress test (NST) performed immediately after a three-second vibroacoustic stimulation with an electronic artificial larynx. A positive response to sound stimulation, recorded as a fetal movement by the mother, occurred on 497 occasions (97.3%) and was accompanied by a reactive NST on 484 occasions; giving a specificity of 99.6 per cent and a negative predictive value of 97.4 per cent. An inconclusive or negative response to sound (2.7%) had a sensitivity of 35.0 per cent and a positive predictive value for a nonreactive NST of 77.8 per cent. Results of sound-provoked fetal movement test and NST, performed within a week of delivery, in 434 women were compared with fetal outcome. The maternal perception of sound-provoked fetal movement test had better specificity (99.1% vs 96.9%), positive predictive value (55.6% vs 35.0%) for poor fetal outcome than the NST, although its sensitivity (50.0% vs 70.0%) and negative predictive value (98.8% vs 99.3%) were lower. Maternal perception of sound-provoked fetal movement test may suffice as an inexpensive and simple method of evaluating antepartum fetal well-being in risk situations. When the mother does not feel any sound provoked fetal movement, NST is then performed. This clinical application can be helpful in a primary health care setting where rapid assessment of fetal health at risk is required.

Acoustic Stimulation↗

PubMedDate not supplied

The effect of the antisperm auto-antibody-bound sperm on in vitro fertilization outcome.

To evaluate the effects of antisperm auto-antibody-bound sperm on the outcome of in vitro fertilization-embryo transfer (IVF-ET), 160 infertile couples undergoing treatment by in vitro fertilization were recruited in this study. In the study group (11 couples, 15 cycles), the male partners were positive for antisperm autoantibodies determined by immunobead test (IBT). In the control group (149 couples, 152 cycles), the men had no such antibodies. The percentages of fertilization rate, cleavage rate and pregnancy rate of the study group and control group wer 75.0 +/- 5.2% vs. 69.3 +/- 2.4%; 82.8% +/- 3.7% and 6.7% +/- 11.8%, respectively. There were no significant differences in in vitro region, type and/or percentage of sperm-bound antibodies also had no effect on the in vitro fertilization outcome. In conclusion, in vitro fertilization-embryo transfer is not significantly affected by antisperm autoantibody-bound sperm determined by immunobead test.

Adult↗

PubMedDate not supplied

Evaluation of the percentage of sperm motility at 24 h and sperm survival ratio for prediction of in vitro fertilization.

The present study was carried out to investigate the predictive value of the percentage of sperm motility after 24-h incubation and sperm survival ratio from semen and inseminated sperm suspension using grading of motility by WHO criteria with respect to the fertilization of oocytes in vitro. A total of 789 oocytes from 85 cases were inseminated and the mean fertilization rate obtained was 72.5%. There was no significant correlation between all of the sperm motility results with fertilization rate in vitro. All sperm motility results were not significantly different between the non-fertilizing group and the fertilizing group and also between the group of fertilization rate < or = 25th percentile (fertilization rate < or = 62.5%) and the group of fertilization rate > 62.5%. However, the initial percentage of rapid progressive sperm motility and progressive motility in semen and inseminated sperm suspension at 24 h gave significant differences between the group of fertilization < or = 50th percentile (fertilization rate < or = 80%) and the group of fertilization rate > 80%. Overall accuracy using these parameters for prediction of fertilization rate > 80% was only about 60%. In conclusion, the percentage of sperm motility at 24 h and sperm survival ratio in both semen and inseminated sperm suspension have no practical value in predicting fertilization rate in vitro. Moreover, detailed motility grading cannot improve the predictivity of these sperm motility parameters.

Cell Survival↗