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N Treviño-García Manzo

Publications and source records attributed to N Treviño-García Manzo.

12 recordsLinked to original sources
PubMed1990

[Localization of Entamoeba histolytica antigens using IgA].

The presence of IgA anti-Entamoeba histolytica antibodies has been demonstrates in intestinal secretions and serum of human amebiasis. We investigated which are the cellular components of trophozoites that react with IgA anti-ameba antibodies from immune serum, colostrum and human milk. The cellular localization of such antigens was accomplished by an indirect immunoperoxidase technique using anti-human IgA (alpha chain specific) labeled with peroxidase, both for light and transmission electron microscopy. Intracellular antigens were localized after permeating the parasites with cold acetone (-10 degrees C) for 3 min. and cryosections of 1 micron thick. The antigens that react with IgA antibodies from immune serum, colostrum and human milk were located in the plasma membrane and the internal portion of some cytoplasmic vesicles. So far, it is unknown what is the biological function of IgA in human amebiasis but in other systems it protects against certain parasites.

Animals↗
PubMed1978

[Ultramicroscopic study of the invasion of the mucosa of the colon by Entamoeba histolytica in axenic cultures].

Animal models have been utilized to study experimentally produced intestinal amebiasis. The guinea pig has been the animal of choice to study the mechanisms by which trophozoites of E. histolytica produce colonic lesions. The purpose of this study was to produce lesions in the colon of newborn guinea pigs by the injection of E. histolytica, HM-1 IMSS, strain grown axenically, and to characterize the early changes with the light and electron microscopes. A total of 35 guinea pigs, one to two days old, were inoculated into the cecum with one and a half million of trophozoites of E. histolytica. Thirteen surviving animals were sacrificed. In four there were clear cut amebic lesions. Sections were taken from neighbouring transitional zones. The most important changes were the migrations of trophozoites to the bottom of colonic glands with many parasites located within the crypts. There was passage of ameabae to the lamina propia with practically no inflammatory reaction. It is thought that the parasite, in contact with epithelial crypt cell, produce direct lysis of these elements leading to migration of amebae into the lamina propia.

Animals↗