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N Tsanev

Publications and source records attributed to N Tsanev.

8 recordsLinked to original sources
PubMed1984

A rapid coagglutination test for the detection and serogrouping of Legionellae.

The applicability of coagglutination for the rapid detection and serogrouping of Legionellae has been investigated. The coagglutination reaction is carried out with the aid of self-made preparations of protein A containing staphylococci, sensitized with specific antibodies against the antigens of L. pneumophila (serogroups I to 6), L. bozemanii and L. micdadei. Preliminary heating of Legionella suspensions at 100% C for 15 min was used to prevent cross coagglutination reactions and ensure greater safety of laboratory personnel during the performance of the test. The results obtained demonstrate a high specificity of coagglutination. With the aid of the coagglutination reactions it has been shown that L. pneumophila strains isolated in Bulgaria belong to serogroup I. The coagglutination method is characterized by its rapidity, simplicity and feasibility. It is a useful and convenient means for the rapid detection and serogrouping of Legionellae.

Agglutination Tests↗
PubMed1982

Lytic activity of Bdellovibrio bacteriovorus against bacteria of the family Legionellaceae.

A lytic activity of Bdellovibrio bacteriovorus strains 6-5-S and 12 was found to be present, against representatives of three Legionella species: Legionella pneumophila-strains Knoxville 1 (serogroup 1), Togus 1 (serogroup 2), Bloomington 2 (serogroup 3) and Los Angeles 1 (serogroup 4); Legionella micdadei-strain Tatlock; Legionella bozemanii-strain Wiga, as well as against strains of Legionella pneumophila isolated in Bulgaria-Draginovo 1, 2, 3-belonging to serogroup 1. It is suggested that B. bacteriovorus participates in the self-purification of the environment from legionellae.

Bacteriolysis↗
PubMed1981

Isolation of Legionella pneumophila in Bulgaria.

Microorganisms have been isolated from water samples obtained from a small warm mineral lake. The growth, staining, biochemical and antigenic properties of the microorganisms suggest that they belong to legionella pneumophila serogroup 1. This is the first isolation of Legionella in Bulgaria. The necessity of careful search for legionellosis in this country was underlined.

Agglutination Tests↗
PubMed1979

Specific staining of bacilli by means of immuno-indian-ink method and immunofluorescent method.

The possibilities for identification of B. anthracis are investigated by means of the Immuno-Indian-ink method (IIIM) and the immunofluorescent method (IFM) in their direct and indirect modifications. The specifity of sera against noncapsulated cells of B. anthrasis increases by their adsorption with antigenically related strains of B. cereus as their vegetative cells and spores are killed beforehand with performic acid. Repeated uses of the bacillus suspension for adsorption is possible by treating it with hydrochloric acid in order to restore its antigenic properties. The adsorbed sera against noncapsulated cells of B. anthracis in the direct and indirect modifications of IIIM and IFM stain specifically the 19 investigated B. anthracis strains but none of the 152 strains of saprophytic bacilli. In addition to that the possibility for differentiation of B. anthracis from saprophytic bacilli is established also by combination of cultivation on selective medium, facilitating the capsule formation of B. anthracis with IIIM and IFM, performed with sera against capsule antigens of the anthrax agent.

Animals↗