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N Tsuda

Publications and source records attributed to N Tsuda.

At least 19 recordsLinked to original sources
PubMed1995

Immunohistochemical detection of alpha 1-antitrypsin, alpha 1-antichymotrypsin, transferrin and ferritin in ameloblastoma.

An immunohistochemical study was performed to investigate the presence of alpha-1-antitrypsin (alpha 1-AT), alpha-1-antichymotrypsin (alpha 1-ACT), transferrin and ferritin in 32 ameloblastomas and to evaluate the co-expression of these antibodies. Histologically, we recognized the following 6 patterns in the series of 32 ameloblastomas and at least 2 patterns in variable proportions were present in each of our cases: follicular pattern (21 cases, 66%), plexiform pattern (17 cases, 53%), cystic pattern (21 cases, 66%), acanthomatous pattern (10 cases, 31%), granular cell type (2 cases, 6%), and hyalinized stromal pattern (20 cases, 63%). Neoplastic epithelia of cystic pattern were divided into superficial cell, basal cell and whole layer to compare the immunohistochemical localization. The results made on the various patterns of ameloblastomas were as follows: (1) alpha 1-AT positivity in plexiform, cystic and hyalinized stromal patterns was significantly higher than that of alpha 1-ACT (P < 0.05). (2) The incidence of transferrin in follicular and plexiform patterns was markedly higher than that of ferritin in the same patterns (P < 0.025 and P < 0.01). Transferrin strongly stained metaplastic squamous cells of acanthomatous pattern and basal cells of cystic epithelium. (3) Granular cells reacted with transferrin and ferritin. (4) In follicular and acanthomatous patterns, coexpression of alpha 1-AT and alpha 1-ACT, alpha 1-AT and transferrin, or alpha 1-ACT and transferrin was higher than that of another combination. On the other hand, co-expression of alpha 1-AT and transferrin in plexiform and cystic patterns was higher than that of other antibodies. These results of co-expression of 4 antibodies used in the present study, suggest that the histogenesis of follicular and acanthomatous patterns is different from that of plexiform and cystic patterns.

Adolescent↗
PubMed1995

[A case of myotonic dystrophy with morning headache following sleep apnea syndrome].

We reported a 39-year-old man with myotonic dystrophy. He suffered from morning headache. Respiratory function tests showed restrictive pattern and arterial gas analysis showed hypoxia and hypercapnia with respiratory acidosis (PaCO2 50.8 mmHg, PaO2 63.8mmHg, pH 7.317, SaO2 89.8%). Polysomnograph showed central apneas exclusively in light sleep (stage 1 and 2). O2 saturation fell at most to as low as 50% during the apneas. We conclude that sleep apnea should be consider in patients with myotonic dystrophy associated with morning headache.

Adult↗
PubMed1995

Infantile digital fibromatosis: a study of the development and regression of cytoplasmic inclusion bodies.

An 8-yr-old Japanese boy developed infantile digital fibromatosis in the right ring finger with recurrence and another lesion in the right middle finger. Histologic investigation of the tumor disclosed that the size and frequency of inclusion bodies were inversely proportional to the degree of fibrosis. Electron microscopic study revealed a variety of stages in the development of inclusion bodies, ranging from small, dense aggregates of filaments into bundles with dense bodies traversing the cytoplasm to typical inclusion bodies that also contained cytoplasmic organelles. In areas of dense fibrosis, the cytoplasm of the tumor cells showed areas of constriction and compression by adjacent bundles of collagen. The tendency for a decrease in the number of inclusion bodies in these areas necessitated a differential diagnosis from other fibrous or fibro-histiocytic lesions. Our findings suggest that the tumor may undergo a decrease in the numbers of inclusion bodies and spontaneously may become fibrotic with time. Thus, even as a form of fibromatosis featuring both recurrence and multiple lesions, it may not have a consistently aggressive nature. These findings support the concept that infantile digital fibromatosis should be managed by limited excision rather than by immediate aggressive surgical treatment.

Child↗
PubMed1995

[A case of secondary carnitine deficiency due to anorexia nervosa and severe liver damage].

We reported an 18-year-old woman with anorexia nervosa and liver damage who showed carnitine deficiency. Her unbalanced diet had little carnitine, and severely damaged liver could not synthesize carnitine. Since total parenteral nutrition (TPN) and tube-feed formulas contain little or no carnitine, the patient had progressive weakness and muscle atrophy. Muscle biopsy revealed variability of fiber diameter, type 1 fiber atrophy and copious amount of lipid granules. Carnitine deficiency must be diagnosed in the early stage by muscle biopsy or muscle carnitine levels, and treated with oral intake of L-carnitine.

Adolescent↗
PubMed1995

[Guillain-Barré syndrome associated with anti-GalNAc-GD1a antibody subsequent to Campylobacter jejuni enteritis].

We report a 15-year-old man with Guillain-Barré syndrome (GBS) following Campylobacter jejuni enteritis. Neurologic examination revealed distal muscle weakness and mild disturbance of superficial sense on right plantar area. Serial electrophysiologic studies indicated that the predominant process was axonal degeneration of motor nerves. Thin-layer chromatography with immunostaining revealed that serum IgG strongly reacted with GalNAc-GD1a and weakly with GM2, but did not react with GM1. This is the first report of GBS subsequent to C. jejuni enteritis associated with anti-GalNAc-GD1a antibody.

Adolescent↗
PubMed1994

Quantification of infarct size by thallium-201 single-photon emission computed tomography using the unfolded map method. Comparison with QRS score and angiographic infarct size at 4 weeks after infarction.

A new technique of data processing, the unfolded map method, was used with thallium-201 single-photon emission computed tomography to quantify infarct size in 35 patients with single-vessel disease at 4 weeks after their first myocardial infarction (24 anterior and 11 inferior infarcts), and the results were compared with those obtained by electrocardiography and contrast left ventriculography. The myocardial borders and the infarcted region were defined using the threshold technique and a cutoff value of 55%. Count profile data for each short-axis slice were unfolded zonally into single planes with the same ratio, and their areas were calculated from the slice thickness and radius. Thus, the size of the unfolded map represented the actual left ventricular myocardial area Infarct size was quantitated from the ratio of pixels in the infarcted region to those in the whole map, and the ratio itself was used as the percent infarct size. Although a defect 1 cm in diameter (0.8 cm2) could not be detected in a phantom study, defects > or = 2 cm in diameter (> or = 3.1 cm2) could be measured satisfactorily. The infarct size and percent infarct size determined by the unfolded map method correlated well with the QRS score (r = 0.841 and r = 0.838), the percentage of abnormally contracting segments on left ventriculography (r = 0.835 and r = 0.877), and the ejection fraction (r = -0.835 and r = 0.856). These data indicate that the unfolded map method provides adequate quantification of infarct size, even in the chronic phase, without complicated data processing.

Adult↗
PubMed1994

Estimation of silver-binding nucleolar organizer regions (AgNORs) in lymphoproliferative disorders of gastrointestinal tract.

We quantified nucleolar organizer regions demonstrable by silver staining technique (AgNORs) in six cases of reactive lymphoid hyperplasia (RLH), and in 14 low-grade B-cell lymphomas (mucosa-associated lymphoid tissue (MALT) 5, centrocytic 3, centroblastic-centrocytic 6) and 49 high-grade B-cell lymphomas (centroblastic 44, immunoblastic 3, Burkitt's one, large cell anaplastic one). The pooled mean AgNOR number in low-grade B-cell lymphomas was significantly higher than that in RLH, and significantly lower than that in high-grade B-cell lymphomas. There was a statistically significant difference between RLH versus centroblastic-centrocytic lymphoma in pooled mean AgNOR number and in RLH versus centrocytic lymphoma but not in RLH versus MALT lymphoma. The AgNOR numbers in the cellular components of RLH were also analyzed. The follicle center centroblasts of RLH exhibited a significantly higher pooled mean AgNOR number than other kinds of lymphoid cells in RLH. Furthermore, AgNOR numbers of gastrointestinal lymphomas were compared with those of cellular elements in RLH. There was a statistically significant increased AgNOR number of centroblastic lymphoma when compared with neoplastic centroblasts in centroblastic-centrocytic lymphoma and follicular centroblasts in RLH. By contrast, there was no significant difference in AgNOR numbers between MALT lymphomas and interfollicular lymphocytes, mantle zone lymphocytes or follicular centrocytes in RLH, respectively. This study demonstrated a similar increase when non-Hodgkin's B-cell lymphomas in gastrointestinal tract were compared with histogenetically related cellular elements of RLH. AgNOR counting might be useful adjunct in the classification and grading of lymphoproliferative disorders in gastrointestinal tract.

Antibodies, Monoclonal↗
PubMed1994

[Clinical analysis of benign transient shuddering-like involuntary movement in elderly people].

We described 9 patients who developed acute onset benign transient shuddering-like involuntary movement in elderly people. There was no consciousness disturbance or sensorimotor dysfunction. There were finger tremor, dysarthria, or gait disturbance in some patients. Asterixis was observed in the upper extremities in 2 patients. Duration of an involuntary movement was less than 1 second, and the series of the involuntary movements continued for several days. This state appeared acutely and disappeared within 0.5 -4 days in the natural course without any sequelae. Oral administration of clonazepam was very effective to suppress this tremulousness. Seven of 9 patients developed recurrence. Although the cause of this syndrome remains unknown, we believe that this condition is clinically important for differential diagnosis of acute onset involuntary movements in elderly people.

Aged↗
PubMed1994

[A case of multiple cerebral infarctions associated with hemichorea induced by amantadine hydrochloride].

We report an 80-year-old woman with hemichorea probably due to amantadine hydrochloride. She visited our hospital because of gait disturbance and decreased mental activity. She was diagnosed as multiple cerebral infarctions and treated with 100 mg/day of amantadine hydrochloride. After two weeks she showed choreic movement of the face and right arm and leg. Haloperidol was administered and her choreic movement disappeared. Brain MRI showed multiple cerebral infarctions in the white matter. There was no lesion in the left basal ganglia. SPECT showed hyperperfusion in the left basal ganglia when choreic movement appeared. After choreic movement disappeared, SPECT showed no asymmetrical blood flow. These findings suggest hemichorea was related to hyperfunction of dopaminergic neurons in the left basal ganglia.

Aged↗
PubMed1994

Nucleolar organizer regions (NORs) in lymphoproliferative disorders of the human salivary gland.

The relationship between the silver-stained proteins of the nucleolar organizer regions (AgNORs) and proliferative activity was studied in 30 patients with lymphoproliferative disorders of the major salivary gland. The salivary gland lesions were microscopically and immunohistochemically differentiated into myoepithelial sialadenitis (MESA, n = 6), low-grade B-cell lymphoma (n = 16), high-grade B-cell lymphoma (n = 5), and T-cell lymphoma (n = 3). The mean AgNOR count per nucleus for MESA was 2.48; for low-grade B-cell lymphoma, 2.19; for high-grade B-cell lymphoma, 6.37; and for T-cell lymphoma, 5.46. There was no significant difference in AgNOR counts per nucleus between the MESA and low-grade B-cell lymphoma, whereas the high-grade B-cell lymphomas exhibited significantly increased mean AgNOR number. Furthermore, the mean numbers of AgNOR were compared with cellular types of lymphoproliferative disorders. A statistically significant difference did exist among AgNOR counts of centroblasts, centrocytes and mantle zone lymphocytes or interfollicular lymphocytes in MESA. There was no significant difference in AgNOR counts per nucleus among the centroblasts of MESA, neoplastic centroblasts of centroblastic/centrocytic lymphoma, and centroblastic lymphoma. It was shown that AgNOR enumeration of tumor cells in mucosa-associated lymphoid tissue (MALT) lymphoma was similar to those seen in mantle zone and interfollicular lymphocytes of MESA. Therefore, we propose that MALT lymphoma may represent a mucosal counterpart of mantle zone lymphocyte- or interfollicular lymphocyte-related low-grade B-cell lymphomas.

Humans↗
PubMed1993

Carcinoma of duodenal bulb arising from the Brunner's gland.

A 75-year-old man was diagnosed as having a sessile tumor measuring 1.4 x 1.0 cm in size in the duodenal bulb after upper gastroduodenal series. The biopsy specimens revealed a proliferation of the adenomatous glands showing an acinar structure with papillary infolding; type III mucus, which is characteristic of Brunner's glands. Antral glands and mucus neck cells of the fundic glands were also observed in the adenomatous glands by concanavalin A staining. Thus, it was clear that the tumor had originated from the Brunner's glands. Three years and four months later, the sessile tumor had developed into a fungating ulcerated tumor via a polypoid form. The biopsy specimens revealed a papillary adenocarcinoma with foci of undifferentiated carcinoma. Retrospectively, the adenomatous glands in the biopsy taken from the sessile tumor should have been regarded as low grade carcinoma. Therefore, we propose that when a polyp or tumor shows an increase in size or change in macroscopic appearance, surgery should be considered.

Adenocarcinoma, Mucinous↗
PubMed1993

Immunophenotypic analysis of extranodal non-Hodgkin's lymphomas in the oral cavity.

Fifty cases of extranodal non-Hodgkin's lymphoma arising in the oral cavity were reclassified using the updated Kiel classification. In order to determine the antigenic phenotype of the proliferating cells in oral lymphoma, we used a panel of paraffin effective antibodies that are known to react with lymphocyte- and histiocyte-associated antigens. The median age of the patients was 53 years, with a male predominance (M:F = 1.9: 1). The great majority of oral non-Hodgkin's lymphomas were B-cell lymphomas. There were 12 low-grade B-cell lymphomas (comprising one mucosa-associated lymphoid tissue, four centrocytic and seven centroblastic-centrocytic lymphomas) and 25 high-grade tumors (comprising 17 centroblastic, two immunoblastic, two Burkitt's and four lymphoblastic lymphomas). All 37 B-cell malignancies showed reactivity for L 26 and KiB 3. A monotypic immunoglobulin staining pattern, as revealed by light chain restriction, was found in 21 cases (57%) of the non-Hodgkin's lymphomas confirming their B-cell origin. Furthermore, monotypic staining for kappa-chain predominated (16/21 kappa, 5/21 lambda). Only a small number (6 cases) was of T-cell lineage and all cases showed positive reaction for UCHL 1, MT 1 and DFT 1. In one of six T-cell lymphomas, Ber-H 2 positive anaplastic large cell lymphoma was detected. Such a case was documented for the first time in the primary extranodal non-Hodgkin's lymphoma of the oral cavity. Five cases could be assigned with certainty to the histiocytic system. These cases were positive for cathepsin D and KP 1 LN 3, which recognized Ia (HLA-DR) antigens, was demonstrated most frequently in high-grade B-cell lymphomas, T-cell lymphomas and true histiocytic lymphomas.

Female↗
PubMed1993

[A case of cough headache with Chiari malformation (type I)].

A 38-year-old woman complained for about 5 years of attacks of headache elicited by coughing, sneezing, and laughing. These attacks became more frequent and more severe. Physical examination showed a short and broad neck. Neurological examination revealed no abnormality. Magnetic resonance imaging demonstrated the Chiari malformation (type I) and the cerebral venous angioma in left frontal white matter. A posterior decompression by suboccipital craniectomy with C1 laminectomy was performed. The headache completely disappeared. Before and after the operation, we measured the intracranial subdural pressure by a small pressure transducer. The raised cranial pressure by the Valsalva's maneuver prolonged for more than one minute. Postoperatively, the raised cranial pressure by the Valsalva's maneuver decreased rapidly. We consider that the cough headache of this patient was associated with a valve-like blockage by the Chiari malformation (type I) at the foramen magunum, resulting in cranio-spinal pressure dissociation by interference with downward pulsation. We believe that there was no correlation between the cough headache and the cerebral venous angioma.

Adult↗
PubMed1993

[Present status of computerization in routine pathology work in Japan: a questionnaire survey and its analysis].

A total of 71.7% of the institutions surveyed were using computers, and most of the institutions not using computers were considering their introduction. Large computers were being used by 36% of the institutions and personal computers (PCs) by 61%. With regard to software, 88% of the large computers had been programmed by professionals, while 76% of the PCs were using commercially available software. The wide difference between the software used by the two types of computer makes it advisable to discuss them separately. The details on the patients at the stage of specimen registration was received on-line in 57% of institutions using large computers but in only 5.7% of those using PCs. Coding had been implemented in 47% of institutions using large computers and in 16% of those using PCs. The rate of use for case reference was 55% for large computers and 44% for PCs. References using a register as well as codes and letter data were also observed. It is considered that a wide-ranging method of reference will probably lead to better ideas than a scrupulous method. In the present survey, 59% of institutions using large computers and 35% of those using PCs replied that system improvements were troublesome. The only way to ensure the privacy of patients and the protection of pathological data is to enhance awareness among medical doctors and paramedical personnel. It is hoped that some organization will lead the way in collecting computer information on pathology duties and make this available free of charge.

Computers↗
PubMed1992

Flow cytometric DNA analysis of vascular soft tissue tumors, including African endemic-type Kaposi's sarcoma.

Vascular tumors of the soft tissue display a wide spectrum of histologic features and biologic behavior. Flow cytometric DNA analysis was performed on 40 vascular tumors, including nine African endemic-type Kaposi's sarcomas, nine angiosarcomas, seven hemangiopericytomas, six glomus tumors, and nine capillary hemangiomas. Six of the nine angiosarcoma cases (67%) and one of the seven hemangiopericytomas cases (14%) were aneuploid. All benign vascular tumors and Kaposi's sarcomas were diploid. Clinically, five of the six angiosarcoma patients with aneuploidy died within 2 to 28 months, while the remaining patient, who had the smallest tumor (2 x 1 cm), survived more than 4 years after the initial diagnosis was made. All three angiosarcoma patients with diploidy died within 10 to 14 months. One hemangiopericytoma patient with aneuploidy died within 1 month. No cases of benign tumor recurred. These results suggest that most vascular tumors, which generally follow a benign clinical course, were diploid and that the majority of those with a poor outcome were aneuploid. However, flow cytometrically assessed DNA ploidy has no prognostic value in angiosarcomas or hemangiopericytomas.

Adolescent↗
PubMed1992

Distribution of tissue markers in acinic cell carcinomas of salivary gland.

Eight cases of acinic cell carcinoma of the salivary glands were histologically reclassified and their immunohistochemical expression and distribution for various tissue antigens were examined. The epithelial elements were divided into tubuloglandular components, microcystic patterns and solid nests. The authors' results indicated the following: 1) The duct luminal cells of tubuloglandular components have distinct epithelial features with cytokeratin (KL 1), alpha 1-antichymotrypsin (alpha 1-ACT), transferrin, lactoferrin, IgA, and carcinoembryonic antigen (CEA) positivity. 2) The cyst-lining cells of microcystic pattern expressed immunophenotypes similar to those of the duct luminal cells. 3) The acinic cells in solid nests had positive results for KL 1, alpha 1-antitrypsin (alpha 1-AT), transferrin, lactoferrin and vasoactive intestinal polypeptide (VIP). 4) The clear cells in solid areas had positive results for KL 1, alpha 1-AT, transferrin and VIP. Both the clear cells and the neoplastic acinic cells showed a rather similar pattern of immunoreactivity. Therefore, the clear cells may transform from the neoplastic acinic cells. 5) Secretory products in tubuloglandular and microcystic patterns had positive results for alpha 1-ACT, lactoferrin, IgA and CEA. 6) The basement membrane-like material between the neoplastic islands has distinct positivity for alpha 1-AT. The result suggests that alpha 1-AT is a useful marker of basement membrane-like material.

Adult↗
PubMed1992

Primary malignant lymphoma of the salivary gland: a tumor of mucosa-associated lymphoid tissue.

The clinical, morphologic and immunohistochemical features of 10 cases of the low-grade mucosa-associated lymphoid tissue (MALT) lymphoma of salivary glands are described. Although the initial histologic diagnosis in nine of these cases was myoepithelial sialadenitis, the diagnosis of primary salivary gland MALT lymphoma was based on the demonstration of light chain restriction and on morphologic characteristics. Histologic study showed a characteristic cytology, which included centrocytoid cells (composed of small centrocytes and monocytoid B cells) and a varying degree of plasma cell differentiation; the occurrence of epithelial or acinar invasion by neoplastic centrocytoid cells; and the presence of reactive lymph follicles among the neoplastic cells. Furthermore, multinucleate giant cells resembling Warthin-Finkeldey cells were detected in seven cases. In the light of these findings, cases previously diagnosed as myoepithelial sialadenitis require careful assessment and nine out of 32 cases are, in reality, examples of primary salivary gland MALT lymphomas. Immunohistochemical analysis of paraffin sections revealed the following characteristic immunophenotype of MALT lymphoma: L26, KiB3 and LN2 positive, and a monotypic immunoglobulin pattern (predominantly IgM/kappa). It was of interest that salivary gland parenchyma, infiltrated by neoplastic centrocytoid cells, reacted with LN3 for cells expressing human leukocyte antigen-DR (HLA-DR) antigens. Whereas salivary gland epithelia devoid of a neoplastic invasion were invariably negative for LN3. This suggests a lymphocyte-mediated role in salivary epithelial HLA-DR expression. It appears that HLA-DR expression is an inducible phenomenon in MALT lymphomas of salivary gland.

Adult↗