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Biomedical subjects

N Tsuji

Publications and source records attributed to N Tsuji.

At least 73 records · Page 4Linked to original sources
PubMed1995

Adenomyoepithelioma of the lung.

A 66-year-old man with an extremely rare neoplasm of the lung, adenomyoepithelioma, is described. The tumor was a well-circumscribed lesion that showed solid, glandular, and papillary growth patterns and was composed of two types of cells, inner epithelial cells and outer myoepithelial cells. This bidirectional differentiation was confirmed immunohistochemically. The inner epithelial cells were positive for carcinoembryonic antigen and epithelial membrane antigen, while the outer myoepithelial cells were S-100 protein-positive. Electron microscopically, the tumor was characterized by a large amount of microfilaments in the cytoplasm and layers of basement membrane-like material in the intercellular spaces. Some glycogen granules were detected.

Adenomyoma↗
PubMed1995

Reversal of tumor necrosis factor resistance in tumor cells by adriamycin via suppression of intracellular resistance factors.

Tumor necrosis factor (TNF) and various chemotherapeutic drugs show synergistic antitumor effects in vitro and in vivo, though the mechanism is not clear. Based on our previous finding that endogenous TNF (enTNF) acts as an intracellular resistance factor against exogenous TNF by scavenging oxygen free radicals (OFR) with induced manganous superoxide dismutase (MnSOD), we examined the suppression of these resistance factors by chemotherapeutic drugs and the resulting increase in TNF cytotoxicity. Pretreatment of HeLa cells, which produce an appreciable amount of enTNF and show apparent TNF resistance, with TNF followed by adriamycin (ADM) resulted in an additive effect, whereas pretreatment with ADM followed by TNF resulted in a synergistic effect. After treatment of HeLa cells with ADM, the expression of enTNF was remarkably suppressed and MnSOD activity was decreased by one-half. These results indicate that suppression of the intracellular resistance factors, i.e., enTNF and MnSOD, by ADM plays an important role in the mechanism of the synergistic antitumor effect of TNF in combination with ADM.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1995

CMV viraemia demonstrated in the serum of a patient with cytomegalovirus pneumonia.

We attempted to demonstrate the expression of cytomegalovirus (CMV) particles in the serum of an acute lymphocytic leukaemia patient with CMV pneumonia. The serum sample was applied to an affinity column coupled with human monoclonal antibody C23 which recognizes the envelope glycoproteins of CMV virus and neutralizes the viral activity. The DNA obtained from each fraction was amplified by double polymerase chain reaction (PCR) and analysed by gel electrophoresis. Bands were clearly observed in the eluted fraction. These results strongly suggest that CMV particles exist in the sera of patients with CMV pneumonia.

Base Sequence↗
PubMed1995

Smoldering gamma delta T-cell granular lymphocytic leukemia associated with pure red cell aplasia.

We present a case of granular lymphocytic leukemia (GLL) associated with pure red cell aplasia (PRCA). Twenty-seven percent of the peripheral blood mononuclear cells (PBMC) were positive for TCR delta 1, but not for delta TCS1 (V delta 2), and a rearrangement of the TCR chain (J delta 1) gene was detected. The PBMC and serum from the patient slightly suppressed CFU-E but not BFU-E colony formation in vitro. These findings suggest that monoclonal proliferation of TCR gamma delta-positive granular lymphocytes (GL) can be associated with PRCA even in a patient having a small proportion of leukemia GL, which we propose to designate as 'smoldering GLL'.

Colony-Forming Units Assay↗
PubMed1995

Insulin receptor-related receptor messenger ribonucleic acid in the stomach is focally expressed in the enterochromaffin-like cells.

The insulin receptor-related receptor (IRR) is a member of the insulin receptor family. In contrast to the widespread expression of insulin receptor and insulin-like growth factor-I receptor messenger RNA (mRNA), the expression of IRR mRNA is highly restricted to the kidney and stomach. IRR mRNA in the kidney is focally expressed in the renal distal tubule cells. However, the cellular localization of IRR mRNA in the stomach remains to be elucidated. Here, we examined the cellular localization of IRR mRNA in the rat stomach by in situ hybridization. IRR mRNA in the stomach was abundantly localized in the basal third of the oxyntic glands of the fundic stomach. IRR mRNA in the stomach was colocalized with mRNA for histidine decarboxylase, a marker for the enterochromaffin-like (ECL) cells, indicating that the expression was restricted to ECL cells. ECL cells actively produce and store histamine, which is an important physiological stimulant of acid secretion from the parietal cells. The preferential localization of IRR mRNA in ECL cells suggests that the IRR plays an important role in the function of these cells.

Animals↗
PubMed1995

Morphological, serological and antigenic characteristics, and protein profile of newly isolated Japanese bovine Babesia parasite with particular reference to those of B. ovata.

An intraerythrocytic large protozoan, tentatively designated Babesia sp. 1, was recently isolated from cattle in Hokkaido Prefecture, Japan. This parasite closely resembled B. ovata in shape of piroplasms, but was distinguishable by other morphological, immunological, and biochemical characters. The paired pyriform piroplasm of B. sp. 1 was larger than that of B. ovata. The results from serological and antigenic examination by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis showed that there were cross- but distinguishable-reaction between B. sp. 1 and B. ovata. Protein profiles of both Babesia parasites piroplasms analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were apparently different from each other. Several major proteins revealed by 2D-PAGE and the immunodominant proteins resolved by Western blot analysis (40 kDa for B. sp. 1 and 29 kDa for B. ovata) were unique to each parasite. The results of the present study indicate the possibility that B. sp. 1 is a species different from B. ovata.

Animals↗
PubMed1995

[Amnestic syndrome after right temporo-occipital subcortical hemorrhage].

A case of right temporo-occipital subcortical hemorrhage with amnestic syndrome was reported. A 65-year-old woman presented with visual disturbance and headache. CT and MRI demonstrated a hemorrhagic lesion in the right occipital lobe involving parahippocampal gyrus. Neurological examination on admission revealed left homonymous hemianopsia and anterograde and retrograde amnesia. Neuropsychological examination revealed marked recent memory disturbance, but she had visual retention and verbal retention, and her value in WAIS (Wechsler Adult Intelligence Scale) was normal. After 40 days, she was discharged without improvement of amnesia. These findings suggest that the cortico-medial temporal lobe pathway was disturbed by subcortical hemorrhage, and we think there are connective fibers between visual and verbal memory systems. This is the first report of hippocampal amnesia following temporo-occipital subcortical hemorrhage.

Aged↗
PubMed1994

Endogenous tumor necrosis factor functions as a resistant factor against adriamycin.

One of the mechanisms of cytotoxicity by tumor necrosis factor (TNF) and heat is the induction of reactive oxygen molecules. Cells producing endogenous tumor necrosis factor (enTNF) show resistance to the cytotoxicity of exogenous TNF and heat by inducing manganous superoxide dismutase (MnSOD) to scavenge the reactive oxygen molecules. Intracellular hydroxyl radical production is also involved in adriamycin-induced cytotoxicity. In this study, we therefore examined the possibility that enTNF may act as a protective protein against adriamycin-induced cytotoxicity in a manner similar to that in which it protects against exogenous TNF and heat. Adriamycin-sensitive L-M (mouse tumorigenic fibroblast) cells, originally expressing no enTNF, were transfected with an expression vector which directs the synthesis of non-secretory-type human TNF (enTNF). The stable transformants became resistant to adriamycin with increased levels of MnSOD. Conversely, when HeLa (human uterine cervical cancer) cells, which originally produce an appreciable amount of enTNF, were transfected with an anti-sense TNF mRNA expression vector to inhibit enTNF synthesis, their intracellular MnSOD activity was suppressed and adriamycin sensitivity was enhanced. However, no alterations in expression of multidrug-resistant gene products--P-170 glycoprotein, glutathione S-transferase pi (GST-pi) and the intracellular concentrations of glutathione (GSH)--were observed in these transfectants as compared to their parent cells. These results indicate that enTNF exerts its intracellular protective effect against adriamycin-induced cytotoxicity by the same mechanism as that against exogenous TNF and heat, namely scavenging reactive oxygen with induced MnSOD.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1994

Simultaneous Assay of ErbB-2 Protein and Carcinoembryonic Antigen in Cyst Fluid as an Aid in Diagnosing Cystic Lesions of the Breast.

C-erbB-2 oncoprotein (ErbB-2 protein)and carcinoembryonic antigen (CEA)were simultaneously evaluated in the breast cyst fluid of 6 patients with intracystic cancer, 6 patients with intracystic papilloma, and 42 patients with gross cystic disease. A combination test, using 12 ng of ErbB-2 protein and 150 ng of CEA/ml of cyst fluid, respectively, as cutoff values resulted in a positive detection rate of 83%(5/6 cases) for intracystic breast cancer. Two of the 6 patients with intracystic papilloma and 2 of the 42 patients with gross cystic disease were positive. In addition, elevated levels of these markers in cyst fluid correlated well with the expression of these antigens in the corresponding tumor. Thus, it can be concluded that measurements of ErbB-2 protein and CEA aid in the diagnosis of intracystic breast cancer, and the assay of ErbB-2 protein is valuable for detecting tumors in which this protein is overexpressed.

Journal Article↗
PubMed1994

Development in vitro of free-living infective larvae to the parasitic stage of Strongyloides venezuelensis by temperature shift.

Free-living infective larvae Strongyloides venezuelensis were cultured in Dulbecco's modified Eagle's medium at 25 and 37 degrees C, and development to the parasitic stage was evaluated using morphological, protein and antigenicity criteria. Few larvae cultured at 25 degrees C showed development whereas, in most of the larvae cultured at 37 degrees C, there appeared characteristic changes such as a bulb-like head and droplets under the cuticle with an increase of body width of the larvae. The results obtained from two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) revealed that the protein spot patterns of the larvae cultured at 25 and 37 degrees C were differentiated by 17 specific spots. In addition, Western blot analysis combined with 2D-PAGE for reaction with serum obtained from an infected rat revealed that protein spots showing immunodominant antigen at 37 degrees C were almost the same as those of the larvae recovered from the rats rather than those of the larvae at 25 degrees C. These results strongly suggested that a temperature shift from 25 to 37 degrees C has an important role in the development of free-living infective larvae to the parasitic stage of S. venezuelensis. The culture system established in the present study was useful for biological and biochemical studies in the development from/of the free-living to the parasitic stage of Strongyloides species.

Animals↗
PubMed1994

Arteriovenous malformation occluded by Masson's vegetant intravascular haemangioendothelioma and complicated with intracerebral haematoma.

A case of arteriovenous malformation occluded by Masson's vegetant intravascular haemangioendothelioma and complicated with intracerebral haemorrhage in an 18-year-old woman is reported. Marked endothelial proliferation was found in the vascular lumen of draining vein. The haemorrhage appeared to have resulted from impaired venous drainage from the arteriovenous malformation.

Adolescent↗
PubMed1994

Enhancement of blood stasis and vascular permeability in Meth-A tumors by administration of hyperthermia in combination with tumor necrosis factor.

Blood stasis and vascular permeability induced by tumor necrosis factor (TNF) in Meth-A tumors transplanted in BALB/c mice were significantly enhanced by hyperthermia at 40 degrees C for 30 min immediately following TNF administration. A dose-dependent, sustained decline in the intratumoral blood flow rate occurred following the administration of TNF alone (i.v.; 5 x 10(3), 5 x 10(4), or 5 x 10(5) JRU/kg) and was enhanced by the administration of hyperthermia in combination with the TNF, even though no decline occurred with hyperthermia alone. The combination of TNF at 5 x 10(5) JRU/kg and hyperthermia resulted in a blood flow ratio (ratio of blood flow after administration to that before) of 0.47 at 1 h compared with a ratio of 0.65 at 1 h after TNF alone. The blood flow in normal skin sites did not decrease in any case. The permeability of the intratumoral vasculature similarly increased in a dose-dependent manner after the administration of TNF alone and was further increased by combination with hyperthermia, even though no increase occurred with hyperthermia alone. The mean permeability in mice receiving TNF alone at 5 x 10(5) JRU/kg was 1.35 times that in untreated mice. In mice receiving TNF at the same dose together with hyperthermia, the ratio was increased to 1.65. The results suggest that TNF selectively suppresses intratumoral blood flow, that this effect is enhanced by mild hyperthermia, and that the mechanism of the suppression by TNF with or without hyperthermia partly involves an increase in blood vessel permeability.

Animals↗
PubMed1994

Induction of synthesis of heat shock protein 72 in tumor necrosis factor gene-transduced cells.

Heat shock protein (HSP) and endogenous tumor necrosis factor (enTNF) both act as resistance factors against the cytotoxicity of various cellular stresses. To clarify the relationship between these two stress response systems, we investigated whether or not enTNF is capable of inducing HSP72. Without heating, no difference was found in HSP72 synthesis between enTNF-nonexpressing L-M cells and cells expressing L-R or L-M (pcDV-TNF). After initiation of heat treatment, however, a remarkable increase in HSP72 synthesis was noted in enTNF-expressing cells compared to enTNF-nonexpressing L-M cells. These findings indicated that enTNF augments heat-inducible HSP72 synthesis.

Animals↗
PubMed1994

Specific interaction of pancreatic elastase and leucocytes to produce oxygen radicals and its implication in pancreatitis.

Many previous reports using experimental animal models of pancreatitis have suggested that oxygen free radicals play an important part in initiation and development of pancreatitis. Infiltration of inflammatory cells--that is, neutrophils, lymphocytes, and monocytes--has been seen in damaged pancreatic glands of animal models and patients with pancreatitis. As neutrophils are known to be the highest producer of oxygen free radicals among these inflammatory cells, it seems plausible that oxygen free radicals produced by neutrophils have some pathoaetiological meaning in pancreatitis. This study measured the superoxide production by neutrophils obtained from patients with acute and chronic pancreatitis and then examined the effects of pancreatic enzymes on superoxide production. Patients showed significantly higher superoxide production by 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA) stimulated neutrophils than healthy controls. Among the three pancreatic enzymes, amylase, trypsin, and elastase, elastase was the only one that increased the superoxide production by PMA stimulated neutrophils, by an increment of 1.5-fold. It also increased the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase prepared from PMA stimulated neutrophils by a factor of 2.1. High affinity and low affinity binding sites for elastase on neutrophils were identified. These results suggest that elastase plays a part in the development of pancreatitis by enhancing superoxide production of neutrophils.

Acute Disease↗
PubMed1994

Wasting condition under normal cardiac rhythms in rabbits following Strongyloides papillosus infection.

Pathogenicity of Strongyloides papillosus to rabbits was investigated by monitoring of food intake, body weight and electrocardiogram. No abnormalities were observed in rabbits infected with 10(4) infective larvae (L3)/kg. Rabbits given 10(5) L3/kg exhibited anorexia after the establishment of patent infections. Four of them died on Days 19-33 having lost 32-44% of initial body weight. The other one animal regained appetite and body weight as fecal egg output decreased. Cardiac rhythms remained normal even in the emaciation state. In lethal cases, cardiac activities finally disappeared through escape beats preceded by sinus arrhythmia as in a fasted control. These results suggest that heavy S. papillosus infection produces a wasting condition in rabbits resulting from anorexia under normal cardiac functions.

Animals↗
PubMed1994

Protein analysis of Anaplasma marginale and Anaplasma centrale by two-dimensional polyacrylamide gel electrophoresis.

Protein compositions of Anaplasma marginale and A. centrale were analyzed by two-dimensional gel electrophoresis. Both species had a major protein which was composed of 3-4 spots. The molecular weights of these two proteins were approximately 39 kDa. However, the position of these proteins in the gels were slightly different when 2 gel maps were superimposed. Five other protein spots were shared by A. marginale and A. centrale, whereas all the other protein spots were appeared to be unique to each of the species.

Anaplasma↗
PubMed1994

Parasitic females of Strongyloides papillosus as a pathogenetic stage for sudden cardiac death in infected lambs.

The present study was aimed at elucidating the responsibility of parasitic females for sudden cardiac death following Strongyloides papillosus infection in calves. A preliminary experiment demonstrated a percutaneous infection with S. papillosus infective larvae to cause sudden cardiac death in lambs as in calves, indicating lambs could serve as a model to study fatal stronglyloidiasis in calves. Parasitic females of S. papillosus were inoculated into the duodenum of lambs. Lambs given live worms developed continuous sinus tachycardia immediately after inoculation, and died of sudden cardiac arrest by ventricular fibrillation through a phase of the disease identical to the case of percutaneous larval infection. The lambs had high fecal egg counts at the time of death. Inoculation of homogenized worms did not produce fatal arrhythmias. These results demonstrate that live parasitic females of S. papillosus in the small intestine are responsible for cardiac dysfunction regardless of the presence or absence of migratory larvae.

Animals↗