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N V Medunitsyn

Publications and source records attributed to N V Medunitsyn.

At least 19 recordsLinked to original sources
PubMed1999

[The safety problems of new vaccines].

The paper characterizes the etiological factors that produce side effects and postvaccinal complications. It presents a classification of the types of sides effects. The study of the immunological safety of vaccines is a new trend in assessing the quality of vaccines and their standardization. Due to the design of new-generation vaccines (gene-engineering, synthetic, DNA vaccines, etc.), their safety gain in their particular significance. It is necessary to make a strict government surveillance over the design, testing of these agents. Possible ways of reducing the adverse effects of vaccines.

Humans↗
PubMed1990

[The immunomodulating action of interferon preparations. 3. Changes in the phagocytic activity of human blood neutrophilic granulocytes under the influence of interferon preparations].

In vitro studies of phagocytosis-stimulating effects of interferon preparations differing in their origin and mode of purification were carried out with blood cells of dermatosis patients and healthy donors. Native preparations and preparations purified by sparing methods (alpha-interferon for intranasal administration and alpha-interferon for injections II) were found the most active: they induced a significant elevation of the activities of individual cells and of the count of actively phagocytizing cells. Phagocytosis-stimulating effect of well purified interferon preparations is essentially reduced. Recombinant alpha 2-interferon preparation has shown no such activity. A dose dependence of the effect of interferon preparations on blood neutrophil phagocytic activity has been revealed. The highest phagocytosis-stimulating effect of the above interferon preparations was observed in patients whose blood polymorphonuclear leukocyte phagocytic activity was essentially reduced.

Adjuvants, Immunologic↗
PubMed1990

[The immunomodulating action of interferon. The change in the rosette-forming activity of human blood lymphocytes under the influence of gamma-interferon and recombinant alpha 2-interferon].

The authors analyze their findings in the study on the effects of interferon preparations (immune gamma-interferon and recombinant alpha 2-interferon) on the ability of blood lymphocytes of dermatoses patients and normal subjects to enter the rosette formation. Preliminary cell treatment with gamma-interferon in vitro essentially influences lymphocytic rosette forming activity, the initial functional activity of cellular receptor system being of paramount importance here. gamma-interferon increased cellular rosette-forming activity and virtually did not influence the unchanged parameters in the patients with lowered values by spontaneous and early active E-RFC. Besides that immune interferon increased the count of 'heavy' rosettes detectable in spontaneous and active E-rosette formation. Recombinant alpha 2-interferon did not essentially alter the blood lymphocyte receptor system.

Adjuvants, Immunologic↗
PubMed1990

[The immunomodulating action of interferon preparations. The effect of medicinal alpha-interferon preparations on the rosette-forming activity of human blood lymphocytes].

Effects of alpha-interferon (alpha-IF) medical preparations on human blood lymphocyte rosette-forming activity was under study. Blood samples from 40 donors and 81 patients with chronic pyoderma, lupus erythematosus, and pemphigus were examined. Interferon preparations alpha-IF for nasal administration and IN-1 and IN-2 (or leukinterferon) for injections were used. The results evidence that IF preparations activities depend on the methods of the preparation synthesis and purification. alpha-IF and leukinterferon most effectively enhanced the lymphocyte ability to spontaneous, early, and active rosette formation. IN-1 was found less active and enhanced only the ability to spontaneous E-rosette formation, but had no effect on early active E-RFC subpopulations. IF preparations elevated the counts of E-RFC anf active RFC only if these levels were lowered; if these values were normal the agents had no effect.

Adjuvants, Immunologic↗
PubMed1989

[Expression of the class II antigens of the major histocompatibility complex on the blood cells of psoriasis patients and its alteration under the influence of interferon preparations].

The immune status, the level of expression of class II HLA histocompatibility antigens in the peripheral blood cells, and the effects on this antigen expression of alpha- and gamma-interferons have been examined in 21 patients with psoriatic arthropathy. Examinations of the immune status have revealed reduced levels of T-lymphocytes, of active E-RFC, of cells forming rosettes with autologous red cells, and an increased count of 0-lymphocytes. The level of class II antigen expression in monocytes, in total lymphocyte population, and in the fraction enriched for B-lymphocytes is much reduced in all the patients suffering from psoriatic arthropathy. The level of these antigens' expression in T-lymphocytes is increased in 2/3 of patients and unchanged in the rest. Intranasal alpha-interferon increased class II antigen expression in the peripheral blood monocytes of patients, whereas gamma-interferon induced no such effect. The stimulating effect of alpha-interferon on the patients' monocytes has been much less manifest than on normal donor monocytes. The antigen expression in the patients' blood lymphocytes exposed to alpha- and gamma-interferons has been virtually the same.

Adult↗
PubMed1989

[Immunoenzyme analysis of the change in the expression of class II antigens of the major histocompatibility complex under the influence of interferon preparations].

The interferons (IFN's) are natural regulators of the immune response. This quality of IFN depends to a large extent on their capacity to change the expression of the antigens of the second (II) class of the Major Histocompatibility Complex. The most effective modulator of the expression of the antigens of the class II MHC is beta-IFN. The aim of this work is to explore with the aid of enzyme-linked immunosorbent assay (ELISA) peculiarities of an action of the preparations alpha- and beta-IFN's of a man on the expression of the antigens of the second class on macrophages of mice with using monoclonal antibodies to the products of subregions I-A and I-E MHC. As a result of exploration we have the following: both preparations IFN's (leukinterferon and beta-IFN) reinforce the expression of the antigens of the second class on the macrophages of mice spleens. With combined influence preparation alpha-IFN and beta-IFN show interweakened effect on the expression of the antigens of the class MHC. Exposed regularities of the expression have the same type for products of subregions I-A and I-E H-2 of mice complex. ELISA is the sensible method of determination of a level of the expression of the antigens of the class II MHC on the cell's populations.

Animals↗
PubMed1989

[Mechanism of the inhibition of proliferation of hapten-modified human and mouse leukemic cells by hapten-specific T-suppressor factor].

T-suppressor-inducer and T-suppressor-effector hybridoma supernatants both regulating the DTH reaction intensity triggered by trinitrophenyl-sulphonic acid (TNPSA) or antigen-dependent proliferation of mice lymph node cells to trinitrophenylated bovine serum albumin, possess and ability to inhibit proliferation of TNPSA-modified human and mice leukaemic cells. This effect is specific, and is caused from antigen binding. This effect is specific, and is caused from antigen binding, idiotype positive T-suppressor factors and is based on a cyclic adenosine monophosphate increase of the concentration in target cells.

Animals↗
PubMed1987

[Model of a stable form of delayed hypersensitivity to chemical allergens].

The injection to mice of a water-soluble form of a chemical allergen (2, 4, 6-trinitrophenylsulfonic acid) in Freund Complete Adjuvant resulted in the development of extremely intensive and persistent (more than 14 weeks) sensibilization which was determined by skin tests. Depending on the challenge, the persistence of skin reactions varied from 48 to 244 hours, but an early component was detected 6 hours after the injection. When this sensibilization method is applied, the effectors of delayed type hypersensitivity are formed in the regional lymph nodes.

Allergens↗
PubMed1986

[Characteristics of 2 types of antigen-binding T suppressors formed during an immune response using T-T hybridomas and their extracts].

Antigen-binding T cells of mice immunized with low doses of syngenic spleen cells modified by 2,4,6-trinitrophenyl sulphonic acid, were fused with BW 5147.3.13 thymoma subclone. Suppressor hybridomas were not identical in their differentiating antigens and functional activity. Extract from Lyt 1-2+ hybridoma suppressed efferent and afferent limbs of delayed type hypersensitivity (DTH) in recipients sensitized with subcutaneous injection of 3 X 10(7) TNP-SC. Extract from Lyt 1+2+ I-hybridoma suppressed only afferent DTH limb. It is suggested that during DTH induction with low doses of hapten-modified cells the generation of different types of antigen-binding DTH T suppressors takes place.

Animals↗
PubMed1986

[Induction of macrophage migration inhibition factor-producing cells in vitro].

The in vitro induction of effector cells mediating delayed type hypersensitivity (DTH) responses was demonstrated during joint cultivation of plastic non-adherent spleen cells of intact CBA mice and adherent spleen cells pretreated with MBSA or OVA. The activity of these effector cells was studied by testing their ability to produce MIF after stimulation by a specific antigen. The formation of MIF-producing cells was observed with the use of low antigen doses (about 50 micrograms/ml). High OVA doses (250 micrograms/ml) were found to suppress the development of MIF-producing cells. MIF-producing cells obtained after either in vitro or in vivo exposure to identical antigens did not differ in their activity.

Animals↗
PubMed1985

[Evans blue as an adjuvant for enhancing delayed hypersensitivity in an experiment].

A modified method has been elaborated for induction of delayed type hypersensitivity (DH) in CBA mice with the use of Evans blue (EB) as adjuvant. This model permitted studying the mechanism of DH development, establishing the dependence of DH on the dose of EB, the dose and type of protein antigen, and realizing passive transfer of DH with the aid of splenocytes from active-synthesized mice. EB is a convenient and effective adjuvant for induction and study of the mechanism of DH development.

Adjuvants, Immunologic↗
PubMed1983

[Induction of T-suppressors of delayed-type hypersensitivity with a background of T-effector formation].

Administration to mice of 10(5) syngeneic splenocytes modified with trinitrobenzene sulfonic acid leads to the formation of a population of T suppressors which are capable to sorb on a specific antigen. In recipients, these cells suppress only one phase of the induction of delayed type hypersensitivity (DTH). Their precursors are sensitive to the action of low doses of cyclophosphamide. The formation of the suppressors in question occurs during the generation of T effectors of DTH. It is suggested that the suppressors described may be attributed to Tc3 which are activated in the lymph nodes as a result of subcutaneous sensitization with antigen, and which are similar to Tc1 but have the Ly 2+ phenotype.

Animals↗
PubMed1979

[Adjuvant properties of transfer factor preparations from human tonsillar lymphocytes].

Low molecular components (Mol. m below 10 000 dalton) of the extract prepared from the human tonsils lymphocytes produced an adjuvant effect on th formation of tuberculin sensitivity in guinea pigs immunized with BCG. This interspecies adjuvant effect was proportional to the dose of the "transfer-factor" preparations administered, depended on the method of their preparation, was expressed in administration of the preparations simultaneously with the immunization, before it or after the tuberculin tests, and under definite conditions was replaced by the immunodepressive effect.

Adjuvants, Immunologic↗