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Biomedical subjects

N V Nesterova

Publications and source records attributed to N V Nesterova.

At least 19 recordsLinked to original sources
PubMedDate not supplied

[Construction of the immunoenzyme test system "IFA-AtEBV-Strip" for detecting antibodies of the Epstein-Barr virus].

The first home immunoenzyme test-system for finding antibodies to the Epstein-Barr virus (EBV) has been created. The test-system laboratory prototype was called "IFA-AtEBV-strip". Production technology has been developed for specific components of immunoenzyme test system and all the analysis stage conditions have been optimized: the antigen sorption on plates, its interaction with the studied serum, finding out of the formed immune complex. The test system "IFA-AtEBV-strip" sensitivity made up 96%, specificity--100%.

Antibodies, Viral↗
PubMedDate not supplied

[Specific immunoglobulins as effective drugs for treatment of virus infections: present state and urgent tasks in Ukraine].

The thesis about importance of specific immunoglobulin preparations in treatment of infectious diseases of viral and microbial etiology, uncontrollable infections, caused by representatives of herpes viruses family in particular, is developed in the paper. The types of immunoglobulin preparations, their advantages, technology peculiarities has been considered; the experience of application, including that of the authors have been analyzed. The necessity and expedience of intensive development of this branch of immunobiotechnology in Ukraine is proved, especially in connection with a high level of infectious morbidity in this country, concrete recommendations are given.

Biotechnology↗
PubMedDate not supplied

[Using the preparation "human immunoglobulin against herpes simplex virus type 1 for intramuscular injections" in the complex therapy of nervous system diseases].

The technology of obtaining of specific immunoglobulin for serotherapy of neuroinfection caused by virus herpes simplex 1 type was developed. The patients presented with the following diseases: arachnoencephalitis, encephalopolyradiculoneuritis, encephalomyelitis, encephalitis, arachnoiditis, polyneuropathy, encephalomyelopolyradiculoneuritis, meningoencephalitis. The study showed good tolerance and safety of the medicine, no adverse effects registered during the study. The assessed median score of the efficacy was 2.8 from 3. The obtained results suggest using the liquid form preparation for intramuscular injection "Immunoglobulin for treatment of neuroinfection caused by virus herpes simplex type 1". The Close corporation "Biofarma" located in Kyiv produces this medicine.

Central Nervous System Viral Diseases↗
PubMedDate not supplied

[Research of modulation of CD95-mediated apoptosis in lymphoblastic MP-1 and BJAB cells infected by adenovirus and Epstein-Barr virus].

Model systems of infecting limphoblastic MP-1 and BJAB cells by Epstein-Barr virus, 5 serotype adenovirus and double infection are developed. A rather high level of accumulation of DNA of these viruses in the cells in dynamics at monoinfection and inhibition interference at multi-infection was shown by PCR method. The influence of virus infection on proliferative activity was studied. The stimulation of cells growth in the system BJAB + EBV was detected, and double infecting inhibited the process by 50%. The 25% difference in development of apoptosis process between cells infected by adenovirus and EBV was established when defining CD95-mediated apoptosis in infected MP-1 cells. The infecting of BJAB cells by viruses had a scarce effect on the processes of spontaneous apoptosis, but the data on CD95-mediated apoptosis at EBV infection testify to inhibition of this process both at a monoinfection, and at a double infection. The work was performed in the framework of the fundamental agreement of Ministry of Education and Science of Ukraine F7/366-2001, and grant INTAS N011-2382.

Adenoviridae↗
PubMedDate not supplied

[Use of the surface plasmon resonance method for the virological investigations].

The literature data of the last decade concerning the use of the surface plasmon resonance method for detecting viral proteins as well as intact viruses has been cited. Also the principle of the method was stated and peculiarities of its use for the virus investigations considered. The conclusion was drawn about rich potentialities of the SPR method for investigation of different aspects of the virus interactions with specific agents as well as structure changes provoked by different external factors. However the given method still has not found sufficient distribution in the field of virology.

Antibodies, Viral↗
PubMedDate not supplied

[Physicochemical characteristics of cyanophages and their components].

The paper presents the results of the study on the physicochemical properties of DNA, polypeptide composition of proteins, as well as features of quaternary structure of virions of cyanophages LPP-IA, LPP-3, LPP-3A, A-1, A-2, and N-2 lysing filamentous and heterocyst cyanobacteria. Such characteristics may be used as hierarchially important signs in compiling the classificational scheme of cyanophages.

Chemical Phenomena↗