[Replacement of large quantities of blood with a gas-carrier medium based on an emulsion of perfluorocarbons].
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Animals↗
Biomedical subjects
N V Shibaev
Publications and source records attributed to N V Shibaev.
[Morphological analysis of the rat organs after massive blood substitution with perfluorotributylamine emulsion].
Four--eight months after 60--70% substitution of the blood of rats with perfluorotributyl amine emulsion (PFTBA) multiple separate and confluent vacuoles containing PFTBA were detected in cells of the macrophage system in the liver, spleen, lymph nodes, bone marrow, and reticular layer of the adrenal cortex. The macrophages containing perfluorocarbon had a low functional activity confirmed histochemically. Accumulations of macrophages at remote intervals after blood substitution are replaced by polymorphocellular granulomas. The morphofunctional properties of parenchymatous cells of the organs outside of these accumulations as well as other organs not accumulating PFTBA remain normal.
Adrenal Glands↗
[A phase study of the glutamate-dependent EEG effects in the alpha- and beta-frequency ranges during the acute and subchronic administration of piracetam to rats].
The glutamatergic component of the piracetam effect upon the EEG frequency spectrum was studied in wakeful rats with electrodes implanted into somatosensory cortex and hippocampus and a cannula in the lateral ventricle. Piracetam at a dose of 400 mg/kg enhanced the EEG activity in the range of 10.4-16.4 Hz in two phases: early (10-40 min) and late (above 50 min). Only the late phase was retained against the background of the NMDA receptor antagonist CPP (0.1 nmole). This stage was also retained upon the subchronic administration of piracetam. The AMPA-receptor agonist quisqualate (5 nmole) enhanced the EEG power in the range of 1.5-5 Hz and decreased the activity at 10.4-16.4 Hz. The AMPA-receptor antagonist glutamate diethyl ester (1 mumole) leveled the agonist effect, but enhanced the piracetam action in the late phase at frequencies in the alpha range.
Alpha Rhythm↗
[The frequency composition of the brain electrical activity in rats after the use of the delta-sleep peptide and its analogs].
Frequency spectra of brain electrograms in the course of 1 h after peripheral and central administration of the delta-sleep peptide (DSIP) or two its analogues were studied in freely moving rats. In autumn series of experiments carried out on 18 animals was revealed the phase action of DSIP being manifested in initial (up to 20 min after the injection) suppression of fast (20-26 Hz) oscillations in electrocorticograms and their augmentation in subsequent intervals. Under the identical conditions analogues of DSIP induced the effects characteristic for different phases of DSIP action. In spring-summer series of experiments carried out in 6 animals was revealed a significant increase of the delta-waves in electrical activity of the Putamen after intraperitoneal injection of DSIP and its first analogue. Under the conditions of intraventricular injection DSIP induced stable augmentation of oscillations in a diapason of 14-16 Hz in the neocortex, and its analogues induced similar changes in a nearby frequency diapason of 9.6-11 Hz.
Animals↗
[Decrease in the intensity of the cellular immune response and nonspecific inflammation upon exposure to extremely high frequency electromagnetic radiation].
The effect of low-intensity extremely high-frequency electromagnetic radiation (EHF EMR, 42.0 GHz, 0.1 mW/cm2, 20 min daily) on cell-mediated immunity and nonspecific inflammatory response in mice was studied. The intensity of cell-mediated immune response in the reaction of delayed-type hypersensitivity and nonspecific inflammation was estimated by a relative increase in the thickness of foot pad after immunization of animals by sheep red blood cells or zymosan. It was shown for the first time that the radiation reduces both immune and nonspecific inflammatory responses. It was shown with the use of models of acute inflammation and full-thickness skin wounds that EHF EMR suppresses the nonspecific inflammatory response but does not influence the duration of the pathological process. We suppose that the basis of the effects revealed is the modification of functional activity of phagocytic cells under the influence of EHF EMR. The results suggest that some therapeutic effects of EHF EMR can be realized via the inhibition of inflammatory processes.
Animals↗