[Comparative characteristics of photographic emulsions for autoradiographic studies].
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Autoradiography↗
Biomedical subjects
N V Uvarova
Publications and source records attributed to N V Uvarova.
[Rapid autoradiographic method using a highly sensitive photoemulsion].
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Autoradiography↗
Total synthesis of antibiotic indolmycin and its stereoisomers.
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Anti-Bacterial Agents↗
[Use of highly sensitive radioautography for determining the optimal conditions for obtaining hybridomas].
To detect the hybrid cells forming as a result of fusion of the mouse myeloma cells with the immunic mouse splenocytes, a radioautographic method was used which involved the application of 3H-hypoxanthine as a labelled precursor of nucleic acids and of high-sensitive UK emulsion for accelerated preparations of autographs. The optimal conditions of hybridization providing for the maximum yield of hybrid cells and hybrid clones in our experiments were: use of polyethylene glycol ("Löba", mol. weight 4000) in 50% (w/v) concentration, of NS-O or X-653 myeloma cells in the ratio of parental cells 1:5 (myeloma cell: splenocyte).
Animals↗