Biomedical subjects

Nahoko Kasai

Publications and source records attributed to Nahoko Kasai.

5 recordsLinked to original sources

PubMed2005

CA2: the most vulnerable sector to bicuculline exposure in rat hippocampal slice cultures.

The vulnerability of the CA2 sector to chronic exposure to bicuculline was investigated in rat hippocampal slice cultures. Selective neuronal cell death was observed only in the CA2 sector after exposure to 6 microM bicuculline for 12 h, but the effect of the cell toxicity extended to the CA3 sector after 24 h. The effect was increased by adding 20 microM roscovitine but was reduced by adding 200 nM omega-agatoxin IVA. Bicuculline also induced a calcium influx into neuronal cells mainly in the CA2 sector. These results suggest that CA2 is the most vulnerable sector to bicuculline exposure in hippocampal slice cultures, and that neuronal cell death in the CA2 sector involves the P/Q-type voltage-dependent calcium channel.

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PubMed2004

[Effect of magnesium on neural activities in vitro].

It has been well known that magnesium ion (Mg(2+)) plays an important role in biological functions, especially in neural activities and functions. However, not so many researches have been carried to this subject. Here we investigated the Mg(2+)effect on neuronal electrical activities together with NMDA receptor and synaptic glutamate release by using Multi-Electrode Array (MEA) and Enzyme modified MEA-based multi-array sensor.

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PubMed2003

A system for MEA-based multisite stimulation.

The capability for multisite stimulation is one of the biggest potential advantages of microelectrode arrays (MEAs). There remain, however, several technical problems which have hindered the development of a practical stimulation system. An important design goal is to allow programmable multisite stimulation, which produces minimal interference with simultaneous extracellular and patch or whole cell clamp recording. Here, we describe a multisite stimulation and recording system with novel interface circuit modules, in which preamplifiers and transistor transistor logic-driven solid-state switching devices are integrated. This integration permits PC-controlled remote switching of each substrate electrode. This allows not only flexible selection of stimulation sites, but also rapid switching of the selected sites between stimulation and recording, within 1.2 ms. This allowed almost continuous monitoring of extracellular signals at all the substrate-embedded electrodes, including those used for stimulation. In addition, the vibration-free solid-state switching made it possible to record whole-cell synaptic currents in one neuron, evoked from multiple sites in the network. We have used this system to visualize spatial propagation patterns of evoked responses in cultured networks of cortical neurons. This MEA-based stimulation system is a useful tool for studying neuronal signal processing in biological neuronal networks, as well as the process of synaptic integration within single neurons.

Action Potentials↗

PubMed2003

[Real-time detection of neurotransmitter release and its spatial distribution].

Neurotransmitters have been well known as information carriers for a long time. Recently, some of the research indicated their neurotoxicity, while some indicated their neurotrophic actions. It is very important to understand the role of neurotransmitters. Glutamate is one of the most important excitatory neurotransmitter in the brain. We developed a novel measurement method for glutamate. The method we describe here is based on the enzyme-mediated electrochemical detection. Glutamate oxidase and horseradish peroxidase were deposited together with polymer-mediator on the electrode. We applied this idea on ITO multi-array electrode and developed a 64 channel multi-array sensor. The sensor permits us to detect glutamate release from multiple regions simultaneously in real time. As it is possible to illustrate the distribution of glutamate release, the sensor could be used not only in the pharmacological field, but also in medical treatment in the near future.

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PubMed2002

Electrochemical monitoring of glutamate release at multiple positions in a rat hippocampal slice.

The continuous monitoring of the distribution of glutamate (Glu), a neurotransmitter released at synaptic terminals, is important in terms of understanding the signal transfer mechanism in the brain. In this study, we monitored the concentration of Glu released at multiple positions in a hippocampal slice continuously, and obtained an approximate Glu distribution by using our electrochemical glutamate sensor array. After confirming our sensor's high sensitivity to Glu, we placed a slice on the array, and measured the currents at selected electrodes in the array. When we stimulated a specific position in the slice electrically, the glutamate concentration increased in different areas after several tens of seconds. The presence of glutamate receptor blockers suppressed these increases. This suggests that the electrical signal was transferred along with neurons through synapses and stimulated the Glu release. Our multichannel glutamate sensor should be a powerful tool to determining the distribution of real-time glutamate non-invasively for the studies using biological samples.

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