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Nancy Diaz

Publications and source records attributed to Nancy Diaz.

2 recordsLinked to original sources

How do internal medicine residency programs evaluate their resident float experiences?

OBJECTIVES: We surveyed the nation's internal medicine residency training program directors to determine the range and frequency of existing methods by which float experiences are evaluated. METHODS: We sent questionnaires to the program directors of all 396 internal medicine residency training program sites in the country. Information requested included program characteristics, months devoted to float experiences in each year of training, and the location and purpose of the rotation. Program directors were also asked to choose among descriptors characterizing the evaluative process. RESULTS: There were 139 responding programs (39%), 134 with data that could be aggregated. Responding programs were similar to all programs nationally in the distributions of size and university sponsorship. Overall, 76% of programs employed a night float for any period of time, and 71% currently had one, on average for 6.7 years. Mean months of float experience during residency was 2.4 months, significantly longer in programs that were not university based. Float experiences were evaluated in 89% of those programs who employed them, with ten different methods reported. University-based programs were significantly less likely to use chart review as a method of evaluation, but no other differences in methodology were significant. CONCLUSIONS: Float rotations are common among internal medicine residency training programs. Evaluative methods vary, but one or more are applied in the vast majority of programs.

Hospital Administration↗

Import of the transfer RNase colicin D requires site-specific interaction with the energy-transducing protein TonB.

The transfer RNase colicin D and ionophoric colicin B appropriate the outer membrane iron siderophore receptor FepA and share a common translocation requirement for the TonB pathway to cross the outer membrane. Despite the almost identical sequences of the N-terminal domains required for the translocation of colicins D and B, two spontaneous tonB mutations (Arg158Ser and Pro161Leu) completely abolished colicin D toxicity but did not affect either the sensitivity to other colicins or the FepA-dependent siderophore uptake capacity. The sensitivity to colicin D of both tonB mutants was fully restored by specific suppressor mutations in the TonB box of colicin D, at Ser18(Thr) and Met19(Ile), respectively. This demonstrates that the interaction of colicin D with TonB is critically dependent on certain residues close to position 160 in TonB and on the side chains of certain residues in the TonB box of colicin D. The effect of introducing the TonB boxes from other TonB-dependent receptors and colicins into colicins D and B was studied. The results of these and other changes in the two TonB boxes show that the role of residues at positions 18 and 19 in colicin D is strongly modulated by other nearby and/or distant residues and that the overall function of colicin D is much more dependent on the interaction with TonB involving the TonB box than is the function of colicin B.

Bacterial Proteins↗