[Polymorphonuclear leukocytes decrease the immunostimulatry capacity of human dendritic cells].
The aim of this work was to evaluate the ability of polymorphonuclear leukocytes (PMNL) to modulate the function of dendritic cells (DCs). Human DCs were used to analyze the effect of PMNL on DCs. The allostimulatory proliferation of T cells induced by DCs incubated with PMNL was lower (42 +/- 14%, n:8, p<0.05) compared with untreated DCs. The decreased proliferation correlated with lower IFNgamma production (DCs: 781 +/- 3 pg/ ml; DCs-CS: 343 +/- 178 pg/ml, p<0.05). The same effect was observed in an antigen specific assay. However, the effect was not observed when PMNL where incubated with mature DCs. Indeed, the effect of PMNL was partially reverted with serine proteases inhibitors. Overall, these results suggest that PMNL modulates DCs activity by serine proteases.