Biomedical subjects

Natalia K. Gorlina

Publications and source records attributed to Natalia K. Gorlina.

4 recordsLinked to original sources

PubMed2001

Alpha2-Macroglobulin Modulates Interactions between Lymphocytes and Fibroblasts.

The aim of the present study was to evaluate the influence of apha2-macroglobulin (alpha(2)M) on lymphocyte adhesion to fibroblasts. Peripheral blood lymphocytes from healthy donors and two fibroblast lines (human diploid embryo fibroblasts M-19 and mouse transformed fibroblasts L929) were used in the experiments. alpha(2)M treatment of fibroblast monolayer appeared to result in the enhancement of lymphocyte adhesion to fibroblasts. The number of attached lymphocytes was increased by 2-2.5 times. It should be noted that the effect of alpha(2)M didn't depend on the conformational molecule changes, since either native or methylamine or plasmin transformed alpha(2)M approximately at the same fashion increased the lymphocyte adhesion to both allogeneic and xenogeneic fibroblasts. B lymphocytes were predominant cells that were attached to fibroblast monolayer without alpha(2)M treatment. However the percentage of adherent T lymphocytes was increased substantially after the fibroblast monolayer treatment by alpha(2)M. Subpopulation analysis has shown that fibroblast pretreatment by alpha(2)M didn't result in a selective adhesion of CD4(+) or CD8(+) T lymphocytes, but increased the adhesiveness for both T lymphocyte subpopulations. The data obtained demonstrate that besides its participation in the processes of fibroblast adhesion alpha(2)M is capable to modify the contact interaction of these cells with lymphocytes that may have an influence on the functional consequences of this process.

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PubMed2001

Pregnancy-Associated Plasma Protein-A, Alpha-2-Macroglobulin, Pregnancy Zone Protein and Their Complexes with IgG in Sera of Healthy Non-Pregnant and Pregnant Woman, and Patients with Breast Cancer.

The purpose of this study was to measure alpha-2-macroglobulin-IgG (alpha(2)-MG-IgG), pregnancy zone protein-IgG (PZP-IgG) and pregnancy-associated plasma protein-A-IgG (PAPP-A-IgG) complex concentrations in serum in comparison with total alpha(2)-MG, PZP and PAPP-A concentration, and to determine the possible role of such complexes. Serum samples were obtained from 20 healthy women, 30 patients with verified metastatic breast cancer (stage III-IV) before the treatment and 40 healthy women with normal pregnancy (I-III trimester in dynamics). The serum concentrations of alpha(2)-MG-IgG, PZP-IgG and PAPP-A-IgG complexes were measured by ELISA. It has been shown that the immune complexes of the major proteinase inhibitors such as alpha(2)-MG, PZP and PAPP-A with IgG may be found at minor concentration in blood of both the healthy women and patients with breast cancer. On the other hand, their physiological functions in organism are absolutely different. We suggest that alpha(2)-MG-IgG and PZP-IgG complexes can serve as immunoregulatory signal molecules. Thus, after calculation of a molar ratio, less than 1% of alpha(2)-MG and PZP were marked by IgG. On the contrary, one PAPP-A molecule can bind to 10-15 IgG molecules in samples of control non-pregnant women and breast cancer patients. Such a kind of binding gives an evidence in favor of alternative way of utilization and degradation of PAPP-A by monocyte-macrophages through a Fc-receptor.

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PubMed1999

Low-Molecular Collagen Peptides Have Different Effects on Peritoneal Macrophages and Neutrophils.

Two types of phagocytes - neutrophils and macrophages, are very important participants in inflammation. However, the roles played by these cells in the regulation of an inflammation are radically different. Neutrophils initiate and ensure the alteration phase. Macrophages, to the contrary, regulate the transition of an inflammation from alternative processes to reparative. During the early stages of an inflammation, under the effect of proteases and free radicals, destruction of collagen proteins occurs and a large number of low-molecular peptides are formed, the concentration of which changes as the inflammatory reaction develops. The object of this work was to study the effect of the total fraction of low-molecular type I collagen peptides on the key functions of neutrophils and macrophages. Under the action of the wide range of concentrations of the collagen peptides (1-1000 &mgr;g/ml), activation of the neutrophil migration into the three-dimensional collagen matrix, amplification of PMA-induced production of free radicals and reduction of apoptosis of those cells were observed. The action of collagen peptides on the functions of macrophages had the opposite effect, i.e. they caused inhibition of the macrophage migration and reduction of PMA-induced production of free radicals. Furthermore, at a concentration of 100 &mgr;g/ml the collagen peptides reliably reduced the apoptosis of macrophages. Thus, collagen peptides are potent regulators of an inflammation, promoting the successive development of its phases through regulation of the functional state of phagocytes.

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PubMed1998

Low Molecular Weight Collagen Peptides Affect Migration, Proliferation and Apoptosis of Mouse Spleen Lymphocytes.

As a consequence of inflammatory tissue degradation collagen proteolysis products may be accumulated in the altered tissue. In this connection, we elaborated a hydrolysis scheme to obtain low molecular weight collagen peptides analogous to those produced in vtiro. To elucidate a possible role of collagen peptides during inflammation their action on lymphocyte migration, proliferation and apoptosis was studied at a wide range of concentrations 1-1000 &mgr;g/ml. The observed effects of peptides were different in three concentration ranges - low (1-50 &mgr;g/ml), middle (50-250 &mgr;g/ml) and high (250-1000 &mgr;g/ml). At high concentrations collagen peptides inhibited lymphocyte migration into 3D collagen matrix, and proliferation, including both spontaneous and stimulated. The middle peptide range induced lymphocyte apoptosis and modulated proliferation. Similar to middle ones, low concentration of collagen peptides modulated lymphocyte proliferation and their effect was the most pronounced. The three concentration ranges may presumably fit different stages of inflammation, since collagen degradation is associated with intensity of tissue alteration. Hence, collagen peptides may control lymphocyte functioning at different inflammation stages. Being naturally produced due to inflammatory tissue degradation, collagen peptides may be considered as complex inflammatory regulator like other traditionally discussed mediators (cytokines, chemokines, lipid mediators, etc.).

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