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Biomedical subjects

Neelam Verma

Publications and source records attributed to Neelam Verma.

5 recordsLinked to original sources

An effective means of biofiltration of heavy metal contaminated water bodies using aquatic weed Eichhornia crassipes.

Various aquatic plant species are known to accumulate heavy metals through the process of bioaccumulation. World's most troublesome aquatic weed water hyacinth (Eichhornia crassipes) has been studied for its tendency to bio-accumulate and bio-magnify the heavy metal contaminants present in water bodies. The chemical investigation of plant parts has shown that it accumulates heavy metals like lead (Pb), chromium (Cr), zinc (Zn), manganese (Mn) and copper (Cu) to a large extent. Of all the heavy metals studied Pb, Zn and Mn tend to show greater affinity towards bioaccumulation. The higher concentration of metal in the aquatic weed signifies the biomagnification that lead to filtration of metallic ions from polluted water. The concept that E. crassipes can be used as a natural aquatic treatment system in the uptake of heavy metals is explored.

Eichhornia↗

An innovative technique for lake management with reference to aeration unit installed at lower lake, Bhopal, India.

Water is key element in human life. All forms of life upon the earth depend upon water for their mere existence. Life & water may be aptly said to be two facets of the same coin. Most of the water bodies are getting polluted due to domestic waste, sewage, industrial waste and agricultural effluent. The present study is designed to ascertain the effectiveness of artificial aeration cum Ozonizer unit installed at Lower Lake, Bhopal for assessment of water quality. Various physico- chemical parameters like pH, Dissolved oxygen, Biochemical Oxygen demand, Chemical oxygen demand, nitrate, phosphate and bacteriological status were studied to assess the extent of deterioration in water quality of Lower lake and at the same time to assess the performance of the dual aeration system in improvement of water quality.

Environmental Monitoring↗

Applications of solid phase microextraction for the determination of metallic and organometallic species.

This paper reviews recent developments of solid phase microextraction (SPME) and its application to the analysis of organometallic species of lead, arsenic, mercury, tin, and selenium by hyphenation with HPLC-GC-atomic spectrometry. In the first part, a background of the technique is given in terms of derivatization, fibers used, extraction and desorption conditions. The second part summarizes typical SPME applications to the determination of organometallic species and the main experimental conditions with the aid of specific examples. Most of the applications comprise alkylation with NaBEt4 and headspace extraction followed by gas chromatographic separation with a suitable detector.

Journal Article↗

Biosensors for heavy metals.

A biosensor is an analytical device that consists of an immobilized biocomponent in conjunction with a transducer, and represents a synergistic combination of biotechnology and microelectronics. This review summarizes the use of biosensors for detecting and quantifying heavy metal ions. Heavy metal contamination is of serious concern to human health since these substances are non-biodegradable and retained by the ecological system. Conventional analytical techniques for heavy metals (such as cold vapour atomic absorption spectrometry, and inductively coupled plasma mass spectrometry) are precise but suffer from the disadvantages of high cost, the need for trained personnel and the fact that they are mostly laboratory bound. Biosensors have the advantages of specificity, low cost, ease of use, portability and the ability to furnish continuous real time signals. The analysis of heavy metal ions can be carried out with biosensors by using both protein (enzyme, metal-binding protein and antibody)-based and whole-cell (natural and genetically engineered microorganism)-based approaches.

Biosensing Techniques↗

A disposable microbial based biosensor for quality control in milk.

The food industry needs suitable analytical methods for quality control, that is, methods that are rapid, reliable, specific and cost-effective as current wet chemistries and analytical practices are time consuming and may require highly skilled labor and expensive equipment. The need arises from heightened consumer concern about food composition and safety. The present study was carried out keeping in view the recently emerging concern of the presence of urea in milk, called "synthetic milk". The biocomponent part of the urea biosensor is an immobilized urease yielding bacterial cell biomass isolated from soil and is coupled to the ammonium ion selective electrode of a potentiometric transducer. The membrane potential of all types of potentiometric cell based probes is related to the activity of electrochemically detected product, and thus to the activity of the substrate by a form of the Nernst equation. Samples of milk were collected and analyzed for the presence of urea by the developed biosensor with a response time as low as 2 min. The results were in good correlation with the pure enzyme system.

Animals↗