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Nevio Cimolai

Publications and source records attributed to Nevio Cimolai.

9 recordsLinked to original sources
PubMed2006

Ocular methicillin-resistant Staphylococcus aureus infections in a newborn intensive care cohort.

PURPOSE: To examine the occurrence and outcome of methicillin-resistant Staphylococcus aureus ophthalmologic infections in a newborn intensive care unit cohort. DESIGN: Noninterventional case series. METHODS: Over a 13-month period, 29 neonates were colonized with methicillin-resistant S. aureus in a newborn intensive care unit outbreak. Patients were followed prospectively during the course of colonization and infection. The frequency and type of ophthalmologic infection was recorded, and patients were treated according to clinical need. RESULTS: Eleven of these patients were found to have purulent conjunctivitis. No serious ocular disease ensued in any of these. Spontaneous resolution occurred in some patients, while others improved after either topical or systemic antibiotic treatment. CONCLUSION: Despite invasive infection caused by methicillin-resistant S. aureus, serious ophthalmologic infection was not found.

Anti-Bacterial Agents↗
PubMed2003

Staphylococcus aureus outbreaks among newborns: new frontiers in an old dilemma.

The acquisition of Staphylococcus aureus by the newborn in the early neonatal period is common. Most such acquisitions are not followed by infection, although the potential for serious infection increases in the intensive care unit setting. Attack rates for infection are quite variable, but outbreaks have occurred on many occasions. Although some bacterial virulence factors, such as exfoliative toxins, are associated with outbreaks, most epidemic strains do not have well-defined virulence factors that can be incriminated. There are many possible control measures, but the implementation of these are balanced by the existing circumstances and perceived cost. As methicillin-resistant S. aureus (MRSA) becomes endemic in hospital settings, these bacteria are becoming much more common in nursery outbreaks. Over time, antibiotic selection and enhanced disease causation will lead to the evolution of hypervirulent MRSA clones. The latter will become an increasing problem unless effectively controlled.

Disease Outbreaks↗
PubMed2002

Anti-Mycoplasma pneumoniae secretory antibody in human breast milk.

The secretory antibody responses to M. pneumoniae polypeptides were assessed among human breast milk samples. M. pneumoniae polypeptides were resolved with conventional SDS-PAGE, and antibody responses were determined by immunoblotting. Antibodies that contained secretory component were found to recognize antigens of 200, 185, 170, 150, 135, 92, 85, 60, 43, 27, and 24 kDa (M(r)). Among the latter, antigens of 170, 150, 60, and 43 kDa were recognized from the majority. Although these antibody binding sites parallel those that are also generally recognized by human sera when equivalent antigen is used, the high frequency (80%) of 150 kDa antigen recognition is not common for serum responses. This antigen should be further explored for its potential in future assessments of component vaccines.

Antibodies, Bacterial↗
PubMed2002

Are all MRSA made equal?

The health and economic burden of methicillin-resistant Staphylococcus aureus (MRSA) in the medical realm is considerable. Although there is ample clinical and laboratory evidence indicating that methicillin-susceptible S. aureus (MSSA) is heterogeneous in disease causation, the same heterogeneity has not been well documented for MRSA. Data from animal models and human studies suggest that MRSA is at least as pathogenic as MSSA. Many comparative clinical studies, mainly retrospective, have assessed the virulence of MSSA and MRSA. Whereas the majority of these studies may be deficient in some aspects of clinical design, there has been a definite trend towards implicating MRSA as the more aggressive pathogen. Such an observation, however, must be tempered with the fact that few such studies have attempted to establish clonality among MRSA isolates. Thus, it is conceivable that hypervirulent clones may represent an important proportion of MRSA from hospital studies where patient-patient spread is likely and, accordingly, comparative studies may be biased. Future clinical studies should be prospective and should use well-defined and homogeneous patient groups. As well, for comparison of MRSA and MSSA, an understanding of clonality is essential.

Animals↗
PubMed1996

Enterobacterial intergenic consensus sequence polymerase chain reaction as a typing method for Burkholderia (Pseudomonas) cepacia.

OBJECTIVE: We developed a rapid polymerase chain reaction (PCR) method, which utilizes primers from an enterobacterial repetitive consensus sequence, to differentiate among strains of Burkholderia cepacia. We then applied this method to isolates from a device-associated pseudoinfection and also compared the discriminative typing potential with that of another PCR-based method. METHODS: A simplified lysis procedure was used to generate DNA substrate for amplification by a PCR method which utilized primer ERIC2 and a reverse complement of ERIC1R. The alternative PCR-based method employed primers which are directed to Bordetella pertussis repetitive sequences. Amplification products were visually assessed after conventional agarose gel electrophoresis and staining. RESULTS: The ERIC-based method appropriately clustered strains for the device-associated pseudo-infection. In addition, there was consistency in the definition of similar or dissimilar strains between the two PCR---based systems. CONCLUSIONS: The ERIC-based PCR typing method offers sufficient discriminatory power for it to be used for epidemiologic purposes.

Journal Article↗
PubMedDate not supplied

Candida dubliniensis fungemia and vascular access infection.

Candida dubliniensis is a newly recognized species of yeast, which may have been forrmerly identified as Candida albicans, that has been rarely isolated from invasive fungal infections among humans. The authors document a C. dubliniensis fungemia that occurred during the course of a vascular access infection in a 2-year-old who was undergoing active therapy for neuroblastoma. Presumptive C. albicans isolates from an 18-year period were reassessed, and it was found that C. dubliniensis is a rare cause of fungemia among pediatric patients (0.5% of all such isolates).

Adolescent↗