Biomedical subjects

Nianqin Jie

Publications and source records attributed to Nianqin Jie.

3 recordsLinked to original sources

PubMed2004

The determination of deoxyribonucleic acids with triadimenol based on the enhancement of resonance light scattering.

For the first time, triadimenol was used to determine nucleic acid (DNA) using the resonance light scattering (RLS) technique. The RLS of triadimenol was greatly enhanced by DNA in the range of pH 1.6 to approximately 1.9. A resonance light-scattering peak at 310 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0 to approximately 9 microg/ml with the detection limit of 24 ng ml(-1). The mechanism studies of the system indicated that the enhanced RLS is due to the aggregation of triadimenol on DNA. The nucleic acids in synthetic samples and in rice seedling extraction were analyzed with satisfactory results. Compared with other methods, this method is convenient, rapid, inexpensive and simple.

DNA↗

PubMed2003

Determination of deoxyribonucleic acids by a resonance light scattering technique and its application.

For the first time, acetamiprid has been used to determine nucleic acid (DNA) using the resonance light scattering (RLS). The RLS of acetamiprid was greatly enhanced by DNA in the range of pH 1.6-1.8. A RLS peak at 313 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-11.0 microg ml(-1) with the detection limit of 20 ng ml(-1). The nucleic acids in synthetic sample and in rice seedling extraction were determined satisfactorily. The interaction mechanism of acetamiprid and DNA is discussed. Mechanism studies show that the enhanced RLS is due to the aggregation of acetamiprid in the presence of DNA.

DNA↗

PubMed2003

Study on the interaction between nucleic acids and acetamiprid.

The interaction between deoxyribonucleic acid (DNA) and acetamiprid was studied. It was found that the fluorescence of acetamiprid could be enhanced in the presence of DNA in sulfuric acid solution. The excitation and emission wavelength of acetamiprid was 291 nm and 587 nm, respectively. Under optimal conditions, the calibration graph is over the range of 0.1-10 micromL(-1). The calibration limit is 0.06 microg mL(-1) (S/N = 3). The determination results of DNA in yeast cell and golden staphylococcus samples by this method were satisfactory. The mechanism of the reaction is discussed.

Calibration↗