Biomedical subjects

Niki Kalariti

Publications and source records attributed to Niki Kalariti.

4 recordsLinked to original sources

PubMed2005

The glutamatergic system outside the CNS and in cancer biology.

Glutamate is a major excitatory neurotransmitter in the CNS. The signalling machinery consists of: glutamate receptors, which are responsible for signal input; plasma glutamate transporters, which are responsible for signal termination; and vesicular glutamate transporters for signal output through exocytic release. Recently, data have suggested that the glutamatergic system plays an important role in non-neuronal tissues. In addition, the expression of glutamatergic system has been implicated in tumour biology. This review outlines the evidence, which suggests that the glutamatergic system may have an important role in cancer biology.

Amino Acid Transport System X-AG↗

PubMedDate not supplied

Glucocorticoid receptor function suppresses insulin-like growth factor 1 activity in human KLE endometrial-like cells.

We analysed the glucocorticoid receptor (GR) regulation on the expression of insulin-like growth factor 1 (IGF-1), type I IGF receptor (IGF-1.R), IGF-binding protein 3 (IGFBP-3), urokinase-type plasminogen activator (uPA) and uPA receptor (uPA.R) mRNA in human KLE endometrial-like cells. We documented that KLE cells express IGF-1, IGF-1.R, uPA and IGFBP-3 mRNA, however not uPA.R mRNA. Exogenous administration of dexamethasone inhibited the proliferation of KLE cells without inducing apoptosis. The inhibition of dexamethasone on KLE cell proliferation was neutralized by exogenous administration of IGF-1. Furthermore, dexamethasone suppressed the expression of IGF-1 mRNA and IGF-1.R mRNA as well as the IGF-1 bioavailability in KLE cell culture media, but it did not alter the expression of uPA mRNA and IGFBP-3 mRNA in KLE cells. Since the peritoneal fluid of women with endometriosis is known to contain IGF-1, which stimulates the proliferation and inhibits the apoptosis of endometrial-like cells, it is conceivable that GR-mediated down-regulation of IGF-1 bioavailability may be of clinical relevance for endometriosis.

Cell Line↗

PubMedDate not supplied

Glutamatergic system in bone physiology.

Bone is a highly innervated tissue consisting of nerve fibers, which contain many neurotransmitters including glutamate. Recently, a number of studies have identified functional glutamate receptors in osteoblasts and osteoclasts, implying that the glutamatergic system has an apparent regulatory role in bone physiology. This review outlines the evidence which suggest that the glutamatergic system regulates bone physiology.

Animals↗

PubMedDate not supplied

Characterization of the glutametergic system in MG-63 osteoblast-like osteosarcoma cells.

BACKGROUND: The glutamate system is a fairly complex bioregulation pathway, consisting of several ionotropic Glu receptors (iGlu.Rs), the metabotropic Glu receptors (mGlu.Rs), Glu transporters (EAATs) and glutamine synthetase (GS), which metabolizes glutamate to glutamine. MATERIALS AND METHODS: We characterized the MG-63 human osteoblast-like osteosarcoma cells with regards to mRNA expression of the (a) NMDA.R group of iGlu.Rs, (b) mGlu.Rs, (c) EAAT1 transporter, and (d) GS, using specific primers for their detection by RT-PCR. RESULTS: Our data confirm the mRNA expression of the NR1, NR2A, NR2B and NR2D subunits of NMDA.R and of GS mRNA in MG-63 cells. In addition, we documented, for the first time, the mRNA expression of the NR3A, EAAT1, mGlu.R1, mGlu.R2, mGlu.R3, mGluR.4, mGlu.R5 and mGlu.R8 mRNA. However, we did not detect the mRNA expression of NR2C, NR3B, mGlu.R6 and mGlu.R7 mRNA. CONCLUSION: Our data suggest that MG-63 cells can be used as a model for studying the possible role of Glu beyond CNS.

Amino Acid Transport System X-AG↗