Biomedical subjects

Noriyoshi Shimizu

Publications and source records attributed to Noriyoshi Shimizu.

13 recordsLinked to original sources

PubMed2007

Aging stimulates cyclooxygenase-2 expression and prostaglandin E2 production in human periodontal ligament cells after the application of compressive force.

BACKGROUND AND OBJECTIVES: Some clinical studies show that alveolar crestal bone loss is higher in adults than in young patients during orthodontic treatment, but the causes of such a phenomenon have not been elucidated. It is known that prostaglandin E2 (PGE2) is a proinflammatory agent and one of the potent osteoclast-inducing factors, and is produced by human periodontal ligament cells in response to orthodontic force. The aim of this study was to investigate age-related change in the biosynthetic capacity of PGE2 and its regulatory gene, cyclooxygenase 2 (COX-2) from periodontal ligament cells in response to mechanical stress. METHODS: Compressive force of 2 g/cm2 was applied for 3-48 h to periodontal ligament cells obtained from human donors aged 9-50 years, and COX-2 mRNA expression in and PGE2 production by the periodontal ligament cells in response to the compressive force were examined. RESULTS: Application of a compressive force of 2 g/cm2 for 3-48 h significantly stimulated these factors in both time- and age-dependent manners. Furthermore, these increases were dramatically larger in periodontal ligament cells obtained from donors over the age of 35. CONCLUSIONS: Periodontal ligament cells obtained from old donors have significantly greater COX-2 expression and PGE2 production in response to compressive force than those from younger donors. The turning point of aging, where significantly larger amounts of theses factors begin production, appears to be around the age of 35. These results may be positively related to the acceleration of alveolar crestal bone loss during orthodontic treatment in adult patients.

Adolescent↗

PubMed2006

Effects of root morphology on stress distribution at the root apex.

It is thought that the stress concentration at the root apex caused by orthodontic force induces root resorption. The purpose of this study was to investigate stress distribution at the root in cases of deviated root shapes using finite element models (FEMs). To clarify this, five three-dimensional FEMs divided by deviated root shape (normal, short, blunt, bent root apex, pipette shape) were constructed and, experimental orthodontic forces, applied in a vertical (intrusive) and horizontal (lingual) direction to the tooth axis. In the short-root model, significant stress was concentrated at the middle of the root. The blunt-shaped root model showed no significant stress concentration at the root. In the models with a bent or pipette-shaped root, significant stress was concentrated at the root apex. During orthodontic force application, stress concentration was observed in the root of the models with short, bent, and pipette-shaped roots, indicating that attention must be paid to root shape during orthodontic treatment.

Alveolar Process↗

PubMed2006

Inclination of the occlusal plane is associated with the direction of the masticatory movement path.

Using lateral cephalograms and a jaw movement-recording system, the relationship between the masticatory movement path and dentofacial morphology was investigated in 17 subjects (9 males and 8 females, mean age 23.5 years) without a history of orthodontic treatment. The masticatory movement path was measured at the right and left lower first molar while the subjects chewed gum. The angle between the Frankfort horizontal plane and the masticatory axis (FH-masticatory angle), defined as the axis passing the opening and closing turning point on the sagittal masticatory path, was also measured. The correlation between the angular measurements derived from the lateral cephalogram and the FH-masticatory angle was then investigated. A positive correlation was observed in the FH-masticatory, occlusal plane (P < 0.05), and mandibular plane (P < 0.01) angles. Furthermore, it was found that the angle between the masticatory axis and the occlusal plane (69.1 +/- 4.2 degrees) remained constant even as the masticatory axis showed a tendency to incline forward as the mandibular plane angle became steeper; the rates of change of the FH-masticatory and the occlusal plane angles were approximately 1:1. This finding suggests that the masticatory movement path is closely associated with the occlusal plane.

Adult↗

PubMed2006

Effect of compressive force on the expression of MMPs, PAs, and their inhibitors in osteoblastic Saos-2 cells.

Bone matrix turnover is regulated by matrix metalloproteinases (MMPs), tissue inhibitors of matrix metalloproteinases (TIMPs), and the plasminogen activation system, including tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), and plasminogen activator inhibitor type-1 (PAI-1). We previously demonstrated that 1.0g/cm(2) of compressive force was an optimal condition for inducing bone formation by osteoblastic Saos-2 cells. Here, we examined the effect of mechanical stress on the expression of MMPs, TIMPs, tPA, uPA, and PAI-1 in Saos-2 cells. The cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and with or without continuously compressive force (0.5-3.0g/cm(2)) for up to 24h. The levels of MMPs, TIMPs, uPA, tPA, and PAI-1 gene expression were estimated by determining the mRNA levels using real-time PCR, and the protein levels were determined using ELISA. The expression levels of MMP-1, MMP-2, MMP-14, and TIMP-1 markedly exceeded the control levels at 1.0g/cm(2) of compressive force, whereas the expression levels of MMP-3, MMP-13, TIMP-2, TIMP-3, TIMP-4, tPA, uPA, and PAI-1 markedly exceeded the control levels at 3.0g/cm(2). These results suggest that mechanical stress stimulates bone matrix turnover by increasing these proteinases and inhibitors, and that the mechanism for the proteolytic degradation of bone matrix proteins differs with the strength of the mechanical stress.

Cell Line, Tumor↗

PubMed2006

Effects of different sizes of occlusal metal on curing depth of light-cured orthodontic band cement.

INTRODUCTION: Sufficient penetration and quantity of light are necessary to cure band cement. It is doubtful whether this occurs when bands are cemented to teeth restored with large pieces of occlusal metal. The purpose of this in-vitro investigation was to examine the curing depth and degree of conversion from monomer to polymer of light-cured adhesives when used to cement bands to ceramic blocks covered with metal restorations. METHODS: Two resin adhesives--Transbond Plus (3M Unitek, Monrovia, Calf) and Ultra Band-Lok (Reliance Orthodontic Products, Itasca, Ill)--and 3 curing methods were used. The upper surfaces of ceramic blocks (13 x 10 x 15 mm), used instead of human molars, were restored by using metal pieces of different sizes (4 x 8 x 2, 6 x 8 x 2, and 8 x 8 x 2 mm). The curing depth of the cement along the lateral surface of the block was measured at the middle of its width with slide calipers. Fourier transform infrared spectrometry was used to evaluate the degree of conversion of the adhesives. RESULTS: There was unpolymerized resin in the deep area of the cement on curing with central irradiation from the occlusal surface. However, the amount of unpolymerized resin was significantly (P < .05) decreased with the boundary and circle irradiation methods. CONCLUSIONS: The curing depth of band cement was significantly affected by the size of the occlusal metal restoration and the irradiation method. Therefore, a suitable irradiation method is essential for complete curing of resin for cementing bands to teeth with large metal restorations.

Absorption↗

PubMed2006

Tapered orthodontic miniscrews induce bone-screw cohesion following immediate loading.

The aim of this study was to investigate the initial stability of tapered orthodontic miniscrews (T-type screws) after placement, the necessity of a healing period, and the propriety of immediate loading. Twenty male Wistar rats with a mean age of 20 weeks were divided into two groups. In the immediate-loading groups, straight orthodontic miniscrews (S-type screws) and T-type screws (five rats each) underwent experimental traction force for 2 weeks (W) immediately after placement. In the healing groups (S- and T-type, five rats each), force was applied for 2 W after a 6-W healing period. The right tibia in each rat was identified as the test limb, while the left tibia in each rat was used as the control group, and underwent no experimental force during the experimental period. The screw-to-bone contact was observed histologically and the bone-screw contact ratio was calculated. Scheffe's test was performed to compare the bone-screw contact ratio in each group using statistical software package (SPSS 8.0 for Windows). In the control group, the bone-screw contact ratio improved from 34.8 +/- 16.0 to 74.8 +/- 12.0 per cent with S-type screws in proportion to the experimental period (2 to 8 W, respectively). With the T-type screws in the test group, there was no significant difference between the immediate-loading and healing groups. In the immediate-loading group, the bone-screw contact ratio with T-type screws was significantly greater (82.3 +/- 15.0 per cent) than with the S-type screws (33.3 +/- 11.8 per cent; P < 0.05), suggesting that T-type screws can be used for orthodontic anchorage immediately after placement.

Animals↗

PubMed2006

Recommended placement torque when tightening an orthodontic mini-implant.

To determine an adequate placement torque for obtaining a better success rate of mini-implants that are screwed into the buccal alveolar bone of the posterior region as an anchor for orthodontic treatment, implant placement torque (IPT) was measured. The subjects were 41 orthodontic patients (124 implants), with an average age of 24.9 years (SD 6.5 years), who had surgery to place titanium mini-implants. The peak value of IPT was measured using a torque screwdriver. The success rate of the mini-implant anchor for 124 implants was 85.5%. The mean IPT ranged from 7.2 to 13.5 N cm, depending on the location of the implants. There was a significant difference in the IPT between maxilla and mandible. The IPT in the mandible was, unexpectedly, significantly higher in the failure group than in the success group. Therefore, a large IPT should not be used always. According to our calculations of the risk ratio for failure, to raise the success rate of 1.6-mm diameter mini-implants, the recommended IPT is within the range from 5 to 10 N cm.

Adolescent↗

PubMed2005

Optimal compressive force induces bone formation via increasing bone morphogenetic proteins production and decreasing their antagonists production by Saos-2 cells.

Orthodontic tooth movement induced alveolar bone resorption and formation around the teeth applied mechanical force. Although mechanical force can promote bone formation, the molecular mechanism that underlies this phenomenon is not fully understood. The purposes of this study were to determine how mechanical stress affects the osteogenic response of human osteoblastic cells (Saos-2), and also to examine the optimal compressive force for osteogenesis in vitro. Saos-2 cells were cultured with or without continuously compressive force (0.5-3.0 g/cm2). The expression of bone morphogenetic proteins (BMPs), their antagonists, and transcription factors which involved in osteogenesis were measured using real-time PCR and/or Western blot analysis. Phosphorylation of Smad1 was determined by Western blot. Loading with 1.0 g/cm2 of compressive force significantly increased the expression of BMPs, Runx2 and osterix. In contrast, the expression of BMP antagonists and AJ18 was decreased with 1.0 g/cm2 of compressive force. Loading with 1.0 g/cm2 of compressive force also induced phosphorylation of Smad1. Noggin inhibited the compressive force-induced phosphorylation of Smad1 markedly, and also partially blocked compressive force-induced Runx2 mRNA expression. Moreover, the conditioned medium from 1.0 g/cm2 of compressive force applied cells apparently increased calcium content in mineralized nodules of Saos-2 culture. This study demonstrates that an optimal compressive force stimulates in vitro mineralization via increasing BMPs production and decreasing their antagonists production.

Bone Morphogenetic Proteins↗

PubMed2005

Optimal compressive force induces bone formation via increasing bone sialoprotein and prostaglandin E(2) production appropriately.

Although orthodontic tooth movement can promote bone formation, the molecular mechanism that underlies this phenomenon is not fully understood. The purposes of this study were to determine how mechanical stress affects the osteogenic response of human osteoblastic cells (Saos-2), and also examine the optimal compression for osteogenesis in vitro. Saos-2 cells cultured with or without continuously compressive force (0.5 approximately 3.0 g/cm(2)). The expression of bone sialoprotein (BSP), osteopontin, and cyclooxygenase-2 (COX-2) were measured using real-time PCR, Western blot analysis and immunoassay. The calcium content in the mineralized nodules was determined using Calcium C-Test kit. Only one loading with 1.0 g/cm(2) of compressive force significantly increased the expression of BSP mRNA and protein, COX-2 mRNA expression and PGE(2) synthesis. Indomethacin, an inhibitor of PGE(2) synthesis, inhibited the compression-induced above phenomenon. Moreover, the conditioned medium from 1.0 g/cm(2) of compressive force apparently stimulated calcium content in mineralized nodules. This study demonstrates that an optimal compressive force stimulates in vitro mineralization by BSP synthesis through the autocrin action of PGE(2) production.

Bone Remodeling↗

PubMed2005

Two- and three-dimensional orthodontic imaging using limited cone beam-computed tomography.

Considerable progress has been made in diagnostic, medical imaging devices such as computed tomography (CT). However, these devices are not used routinely in dentistry and orthodontics because of high cost, large space requirements and the high amount of radiation involved. A device using computed tomography technology has been developed for dental use called a limited cone beam dental compact-CT (3DX). The aim of this article is to demonstrate the usefulness of 3DX imaging for orthodontic diagnosis and treatment planning. We present three cases: (1) one case shows delayed eruption of the upper left second premolar, (2) the second case shows severe impaction of a maxillary second bicuspid; and (3) the third case shows temporomandibular joint disorder (TMD). In the tooth impaction cases, the CT images provided more precise information than conventional radiographic images such as improved observation of the long axis of the tooth, root condition, and overlap with bone. In the TMD case, clear and detailed temporomandibular joint images were observed and pre- and posttreatment condylar positions were easily compared. We conclude that 3DX images provide useful information for orthodontic diagnosis and treatment planning.

Adolescent↗

PubMed2005

Permissible limit for mandibular expansion.

In recent years, mandibular expansion has been increasingly performed in conjunction with orthodontic treatment. Lateral tipping of the molars associated with mandibular expansion should, however, be considered, because excessive expansion may result in excessive buccal tooth inclination, which may disturb the occlusal relationship. This study was conducted to quantitatively clarify molar movement during mandibular expansion using the Schwarz appliance to determine the permissible limit of mandibular expansion as a clinical index for inclination movement. Inclinations in the masticatory surface of the first molar and intermolar width were measured before expansion (T1), after expansion (T2), and before edgewise treatment (T3). Lower plaster models from 29 subjects treated with expansion plates were used and compared with models from 11 control subjects with normal occlusion. The average treatment change (T1-T2) in intermolar width was 5.42 mm (standard deviation 1.98), and the average angle of buccal tooth inclination was 10.16 degrees (standard deviation 3.83). No significant correlation was found between age prior to treatment and the treatment period when they were compared with the intermolar width increments and inclination angles. There was a significant positive correlation between retention duration and the amount of expansion. The regression coefficient of the angle of buccal tooth inclination during expansion to the increment of the intermolar width was approximately 0.2. This means that 1 mm of expansion is accompanied by 5 degrees of molar lateral tipping. This coefficient is clinically useful for estimating the permissible limit for mandibular expansion.

Analysis of Variance↗

PubMed2005

Biomechanical effect of abutment on stability of orthodontic mini-implant. A finite element analysis.

The biomechanical influences of primary factors on titanium mini-implant, which is used as an anchorage for orthodontic tooth movement, were quantified using the three-dimensional finite element method. Six types of finite element models were designed to show various thread pitches from 0.5 to 1.5 mm. Three models were designed with abutment and three other models without abutment. A traction force of 2 N was applied to the head of the mini-implant or abutment to be at 45 degrees to the bone surface. No remarkable differences were observed in the stress distribution patterns regardless of thread pitch variance. However, the stress distribution was remarkably different between models with abutment and without abutment. The maximum stress of the model with abutment and thread pitch 0.5 mm was the least as compared with the other models. Areas of high-level stress were obviously smaller than in the models without abutment. The plots of the displacement distributions of the models with abutment also presented significant pattern differences as compared with the models without abutment. The high-level area was localized to the head of the implant and the abutment in models with abutment. Therefore, the existence of the abutment is significantly useful in decreasing the stress concentration on the bone, while the effect of thread pitch was uncertain.

Biomechanical Phenomena↗

PubMed2003

Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells.

OBJECTIVE: The purpose of this study was to determine the effect of pulse frequencies of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells in vitro. BACKGROUND DATA: Various photo-biostimulatory effects of LLLT, including bone formation, were affected by some irradiation factors such as total energy dose, irradiation phase, laser spectrum, and power density. However, the effects of pulse frequencies used during laser irradiation on bone formation have not been elucidated. MATERIALS AND METHODS: Osteoblast-like cells isolated from fetal rat calvariae were irradiated once with a low-energy Ga-Al-As laser (830 nm, 500 mW, 0.48-3.84 J/cm2) in four different irradiation modes: continuous irradiation (CI), and 1-, 2-, and 8-Hz pulsed irradiation (PI-1, PI-2, PI-8). We then investigated the effects on cellular proliferation, bone nodule formation, alkaline phosphatase (ALP) activity, and ALP gene expression. RESULTS: Laser irradiation in all four groups significantly stimulated cellular proliferation, bone nodule formation, ALP activity, and ALP gene expression, as compared with the non-irradiation group. Notably, PI-1 and -2 irradiation markedly stimulated these factors, when compared with the CI and PI-8 groups, and PI-2 irradiation was the best approach for bone nodule formation in the present experimental conditions. CONCLUSION: Since low-frequency pulsed laser irradiation significantly stimulates bone formation in vitro, it is most likely that the pulse frequency of LLLT an important factor affecting biological responses in bone formation.

Animals↗